Genomic instability induced by a-pinene in Chinese hamster cell line

Fabio Caradonna, Giulia Sciandrello, Irene Catanzaro, Giuseppa Barbata, Marghereth Saverini, Maurizio Mauro

Risultato della ricerca: Article

19 Citazioni (Scopus)

Abstract

Here, we report the effects of exposure of mammalian cells to a-pinene, a bicyclic monoterpene used in insecticides, solvents and perfums. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 mM) of a-pinene, indicateda statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 mM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles.Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well as endoreduplicated cells and chromosome breaks. Clastogenic damage was prevalent over aneuploidogenic damage as demonstred by the higher proportion of kinetochore-negative micronuclei. Alkaline comet confirmed that monoterpene exposure caused DNA lesions in a concentration-dependent manner. This damage probably arose by increased reactive oxygen species (ROS) production. In order to assess the generation of ROS, the cells were incubated with CM-H2DCFDA and thenanalysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after a-pinene treatment indicating increased oxidative stress. On the whole, these findings strongly suggest that a-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through ROS production.
Lingua originaleEnglish
pagine (da-a)1-7
Numero di pagine7
RivistaDefault journal
Volume1
Stato di pubblicazionePublished - 2012

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Monoterpenes
Genomic Instability
Cricetulus
Reactive Oxygen Species
Cells
Cell Line
Oxidative stress
Flow cytometry
DNA
Chromosomes
Insecticides
Genes
Fluorescence
Kinetochores
Chromosome Breakage
Cytogenetic Analysis
Metaphase
Mitosis
DNA Damage
Flow Cytometry

All Science Journal Classification (ASJC) codes

  • Genetics
  • Toxicology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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Genomic instability induced by a-pinene in Chinese hamster cell line. / Caradonna, Fabio; Sciandrello, Giulia; Catanzaro, Irene; Barbata, Giuseppa; Saverini, Marghereth; Mauro, Maurizio.

In: Default journal, Vol. 1, 2012, pag. 1-7.

Risultato della ricerca: Article

Caradonna, Fabio ; Sciandrello, Giulia ; Catanzaro, Irene ; Barbata, Giuseppa ; Saverini, Marghereth ; Mauro, Maurizio. / Genomic instability induced by a-pinene in Chinese hamster cell line. In: Default journal. 2012 ; Vol. 1. pagg. 1-7.
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AU - Caradonna, Fabio

AU - Sciandrello, Giulia

AU - Catanzaro, Irene

AU - Barbata, Giuseppa

AU - Saverini, Marghereth

AU - Mauro, Maurizio

PY - 2012

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N2 - Here, we report the effects of exposure of mammalian cells to a-pinene, a bicyclic monoterpene used in insecticides, solvents and perfums. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 mM) of a-pinene, indicateda statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 mM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles.Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well as endoreduplicated cells and chromosome breaks. Clastogenic damage was prevalent over aneuploidogenic damage as demonstred by the higher proportion of kinetochore-negative micronuclei. Alkaline comet confirmed that monoterpene exposure caused DNA lesions in a concentration-dependent manner. This damage probably arose by increased reactive oxygen species (ROS) production. In order to assess the generation of ROS, the cells were incubated with CM-H2DCFDA and thenanalysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after a-pinene treatment indicating increased oxidative stress. On the whole, these findings strongly suggest that a-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through ROS production.

AB - Here, we report the effects of exposure of mammalian cells to a-pinene, a bicyclic monoterpene used in insecticides, solvents and perfums. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 mM) of a-pinene, indicateda statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 mM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles.Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well as endoreduplicated cells and chromosome breaks. Clastogenic damage was prevalent over aneuploidogenic damage as demonstred by the higher proportion of kinetochore-negative micronuclei. Alkaline comet confirmed that monoterpene exposure caused DNA lesions in a concentration-dependent manner. This damage probably arose by increased reactive oxygen species (ROS) production. In order to assess the generation of ROS, the cells were incubated with CM-H2DCFDA and thenanalysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after a-pinene treatment indicating increased oxidative stress. On the whole, these findings strongly suggest that a-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through ROS production.

KW - Genomic instability

KW - a-pinene

KW - hamster cell lines

UR - http://hdl.handle.net/10447/63337

UR - http://www.ncbi.nlm.nih.gov/pubmed/22379123

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