Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 1-7 |
| Numero di pagine | 7 |
| Rivista | Default journal |
| Volume | 1 |
| Stato di pubblicazione | Published - 2012 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Toxicology
- Genetics(clinical)
- Health, Toxicology and Mutagenesis
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Genomic instability induced by a-pinene in Chinese hamster cell line. / Caradonna, Fabio; Sciandrello, Giulia; Catanzaro, Irene; Barbata, Giuseppa; Saverini, Marghereth; Mauro, Maurizio.
In: Default journal, Vol. 1, 2012, pag. 1-7.Risultato della ricerca: Article
}
TY - JOUR
T1 - Genomic instability induced by a-pinene in Chinese hamster cell line
AU - Caradonna, Fabio
AU - Sciandrello, Giulia
AU - Catanzaro, Irene
AU - Barbata, Giuseppa
AU - Saverini, Marghereth
AU - Mauro, Maurizio
PY - 2012
Y1 - 2012
N2 - Here, we report the effects of exposure of mammalian cells to a-pinene, a bicyclic monoterpene used in insecticides, solvents and perfums. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 mM) of a-pinene, indicateda statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 mM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles.Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well as endoreduplicated cells and chromosome breaks. Clastogenic damage was prevalent over aneuploidogenic damage as demonstred by the higher proportion of kinetochore-negative micronuclei. Alkaline comet confirmed that monoterpene exposure caused DNA lesions in a concentration-dependent manner. This damage probably arose by increased reactive oxygen species (ROS) production. In order to assess the generation of ROS, the cells were incubated with CM-H2DCFDA and thenanalysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after a-pinene treatment indicating increased oxidative stress. On the whole, these findings strongly suggest that a-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through ROS production.
AB - Here, we report the effects of exposure of mammalian cells to a-pinene, a bicyclic monoterpene used in insecticides, solvents and perfums. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 mM) of a-pinene, indicateda statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 mM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles.Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well as endoreduplicated cells and chromosome breaks. Clastogenic damage was prevalent over aneuploidogenic damage as demonstred by the higher proportion of kinetochore-negative micronuclei. Alkaline comet confirmed that monoterpene exposure caused DNA lesions in a concentration-dependent manner. This damage probably arose by increased reactive oxygen species (ROS) production. In order to assess the generation of ROS, the cells were incubated with CM-H2DCFDA and thenanalysed by flow cytometry. Results demonstrated an increase in fluorescence intensity after a-pinene treatment indicating increased oxidative stress. On the whole, these findings strongly suggest that a-pinene is able to compromise genome stability preferentially through mitotic alterations and to damage DNA through ROS production.
KW - Genomic instability
KW - a-pinene
KW - hamster cell lines
UR - http://hdl.handle.net/10447/63337
UR - http://www.ncbi.nlm.nih.gov/pubmed/22379123
M3 - Article
VL - 1
SP - 1
EP - 7
JO - Default journal
JF - Default journal
ER -