Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Simona De Grazia, Jelle Matthijnssens, Maria Assunta Cafiero, Rafael Baselga, Viviana Parreño, Max Ciarlet, Vito Martella, Canio Buonavoglia, Christian Hoffmann, Eleonora Lorusso, Krisztián Bányai

Risultato della ricerca: Articlepeer review

37 Citazioni (Scopus)

Abstract

An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity(95–98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.
Lingua originaleEnglish
pagine (da-a)250-265
Numero di pagine16
RivistaVirus Genes
Volume37
Stato di pubblicazionePublished - 2008

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology

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