The recovery of ancient germplasm intomato (Solanum lycopersicon L.) has become necessaryto limit the wide genetic erosion caused by theemployment of modern cultivars. Among germplasmcollections, long shelf-life landraces could represent animportant source of biodiversity. The present studyprovides a first set of molecular and phenotypic data onlong shelf-life (so called ‘‘da serbo’’ in southern Italy)tomato collection, mainly originated from Sicilytogether with some landraces from Campania andApulia. The analysis of fruit traits showed a low intravarietalvariation, while exhibiting a quite higher intervarietalvariability. Overall, the cultivars have beenclassified in six fruit shape classes of which flattenedand slightly flattened included the 54.76 % of thecollection. The principal component analysis (PCA)showed a large cluster in which almost all landracesfrom Sicily were included. The microsatellite (SSR)analysis confirmed a low intra-varietal variation, andthe very low heterozygosity (Ho) revealed a high degreeof homozygosity in these landraces. In accordance withlimited morphological variability, the values of microsatellitepolymorphism (PIC) showed a low geneticvariability among these long shelf-life tomato cultivars.Cluster analysis based on 10 polymorphic SSR was notable to distinguish landraces for their different origin,while allowed to classify similar genotypes in fourgroups. Three groups showed a limited genetic distancewhile in a fourth largest and genetic variable cluster wasincluded genotypes more selectable for traits of agronomicinterest. Overall, the phenotypic and geneticvariation allowed us to classify a collection of Sicilianlong shelf-life tomato landraces.
|Numero di pagine||12|
|Rivista||Genetic Resources and Crop Evolution|
|Stato di pubblicazione||Published - 2014|
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