Genetic structure in the paleoendemic and endangered Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae) and implications for its conservation

Lorenzo Antonino Gianguzzi, Lorenzo Gianguzzi, Antonietta Di Maio, Filomena Sepe, Olga De Castro, Paola Cennamo, Bruno Menale, Paolo De Luca

Risultato della ricerca: Article

12 Citazioni (Scopus)

Abstract

Our investigation aims to understand the genetic structure and evolutionary history of Petagnaea gussonei, an ancient and endangered species belonging to the Saniculoideae subfamily (Apiaceae). It is paleoendemic to Sicily, with a small number of populations in the Nebrodi Mountains. Atotal of seven chloroplast microsatellite repeat loci and 12 AFLP primer combinations were used to screen 115 individualscorresponding to 17 populations. The ratio of seed to pollen flow was also calculated using the modified Ennos equation. A relatively high level of genetic diversity was detected with AFLPs (e.g., 0.045\H\0.278), and a moderate variation was also found using cpSSRs (0\Hk\0.667). Two different haplotypes (B and W) wereidentified, with five populations being monomorphic for haplotype B. There was no genetic differentiation on the basisof haplotypic frequency (GST) and similarity (RST), and no phylogeographic structure was detected among the populations. AFLP values also confirmed that the populations arenot very genetically differentiated. The principal component analysis based on pairwise genetic differences showed threegroupings without a geographical correlation. The AMOVA analysis indicates that theamount of variation is higher withinpopulations (82 %) than among populations (18 %). Results of the pollen flow/seed flow ratio indicated positive values foreach population, indicating that gene flow by seed is not more efficient than by pollen. Instead, the total pollen/seed flow for all population presents a negative value, suggesting that pollen dispersal does not appear to be more effective over thelong range for gene flow than seed dispersal. This differentiation level supports the hypothesis that the fragmentation and isolation of the residual populations is in progress. Thisphenomenon is due not only to post-ice age climate changes, but also to direct and indirect anthropic actions.
Lingua originaleEnglish
pagine (da-a)209-223
Numero di pagine15
RivistaPlant Systematics and Evolution
Volume299
Stato di pubblicazionePublished - 2013

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Apiaceae
genetic structure
pollen
seed
pollen flow
gene flow
amplified fragment length polymorphism
seeds
seed dispersal
endangered species
chloroplast
genetic differentiation
haplotypes
principal component analysis
fragmentation
Pleistocene
genetic variation
climate change
Sicily
mountain

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

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Genetic structure in the paleoendemic and endangered Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae) and implications for its conservation. / Gianguzzi, Lorenzo Antonino; Gianguzzi, Lorenzo; Di Maio, Antonietta; Sepe, Filomena; De Castro, Olga; Cennamo, Paola; Menale, Bruno; De Luca, Paolo.

In: Plant Systematics and Evolution, Vol. 299, 2013, pag. 209-223.

Risultato della ricerca: Article

Gianguzzi, Lorenzo Antonino ; Gianguzzi, Lorenzo ; Di Maio, Antonietta ; Sepe, Filomena ; De Castro, Olga ; Cennamo, Paola ; Menale, Bruno ; De Luca, Paolo. / Genetic structure in the paleoendemic and endangered Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae) and implications for its conservation. In: Plant Systematics and Evolution. 2013 ; Vol. 299. pagg. 209-223.
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title = "Genetic structure in the paleoendemic and endangered Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae) and implications for its conservation",
abstract = "Our investigation aims to understand the genetic structure and evolutionary history of Petagnaea gussonei, an ancient and endangered species belonging to the Saniculoideae subfamily (Apiaceae). It is paleoendemic to Sicily, with a small number of populations in the Nebrodi Mountains. Atotal of seven chloroplast microsatellite repeat loci and 12 AFLP primer combinations were used to screen 115 individualscorresponding to 17 populations. The ratio of seed to pollen flow was also calculated using the modified Ennos equation. A relatively high level of genetic diversity was detected with AFLPs (e.g., 0.045\H\0.278), and a moderate variation was also found using cpSSRs (0\Hk\0.667). Two different haplotypes (B and W) wereidentified, with five populations being monomorphic for haplotype B. There was no genetic differentiation on the basisof haplotypic frequency (GST) and similarity (RST), and no phylogeographic structure was detected among the populations. AFLP values also confirmed that the populations arenot very genetically differentiated. The principal component analysis based on pairwise genetic differences showed threegroupings without a geographical correlation. The AMOVA analysis indicates that theamount of variation is higher withinpopulations (82 {\%}) than among populations (18 {\%}). Results of the pollen flow/seed flow ratio indicated positive values foreach population, indicating that gene flow by seed is not more efficient than by pollen. Instead, the total pollen/seed flow for all population presents a negative value, suggesting that pollen dispersal does not appear to be more effective over thelong range for gene flow than seed dispersal. This differentiation level supports the hypothesis that the fragmentation and isolation of the residual populations is in progress. Thisphenomenon is due not only to post-ice age climate changes, but also to direct and indirect anthropic actions.",
keywords = "AFLP, Conservation, CpSSR, Genetic structure, Paleoendemic, Petagnaea gussonei, Seed/pollen flow, Sicily",
author = "Gianguzzi, {Lorenzo Antonino} and Lorenzo Gianguzzi and {Di Maio}, Antonietta and Filomena Sepe and {De Castro}, Olga and Paola Cennamo and Bruno Menale and {De Luca}, Paolo",
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language = "English",
volume = "299",
pages = "209--223",
journal = "Plant Systematics and Evolution",
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TY - JOUR

T1 - Genetic structure in the paleoendemic and endangered Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae) and implications for its conservation

AU - Gianguzzi, Lorenzo Antonino

AU - Gianguzzi, Lorenzo

AU - Di Maio, Antonietta

AU - Sepe, Filomena

AU - De Castro, Olga

AU - Cennamo, Paola

AU - Menale, Bruno

AU - De Luca, Paolo

PY - 2013

Y1 - 2013

N2 - Our investigation aims to understand the genetic structure and evolutionary history of Petagnaea gussonei, an ancient and endangered species belonging to the Saniculoideae subfamily (Apiaceae). It is paleoendemic to Sicily, with a small number of populations in the Nebrodi Mountains. Atotal of seven chloroplast microsatellite repeat loci and 12 AFLP primer combinations were used to screen 115 individualscorresponding to 17 populations. The ratio of seed to pollen flow was also calculated using the modified Ennos equation. A relatively high level of genetic diversity was detected with AFLPs (e.g., 0.045\H\0.278), and a moderate variation was also found using cpSSRs (0\Hk\0.667). Two different haplotypes (B and W) wereidentified, with five populations being monomorphic for haplotype B. There was no genetic differentiation on the basisof haplotypic frequency (GST) and similarity (RST), and no phylogeographic structure was detected among the populations. AFLP values also confirmed that the populations arenot very genetically differentiated. The principal component analysis based on pairwise genetic differences showed threegroupings without a geographical correlation. The AMOVA analysis indicates that theamount of variation is higher withinpopulations (82 %) than among populations (18 %). Results of the pollen flow/seed flow ratio indicated positive values foreach population, indicating that gene flow by seed is not more efficient than by pollen. Instead, the total pollen/seed flow for all population presents a negative value, suggesting that pollen dispersal does not appear to be more effective over thelong range for gene flow than seed dispersal. This differentiation level supports the hypothesis that the fragmentation and isolation of the residual populations is in progress. Thisphenomenon is due not only to post-ice age climate changes, but also to direct and indirect anthropic actions.

AB - Our investigation aims to understand the genetic structure and evolutionary history of Petagnaea gussonei, an ancient and endangered species belonging to the Saniculoideae subfamily (Apiaceae). It is paleoendemic to Sicily, with a small number of populations in the Nebrodi Mountains. Atotal of seven chloroplast microsatellite repeat loci and 12 AFLP primer combinations were used to screen 115 individualscorresponding to 17 populations. The ratio of seed to pollen flow was also calculated using the modified Ennos equation. A relatively high level of genetic diversity was detected with AFLPs (e.g., 0.045\H\0.278), and a moderate variation was also found using cpSSRs (0\Hk\0.667). Two different haplotypes (B and W) wereidentified, with five populations being monomorphic for haplotype B. There was no genetic differentiation on the basisof haplotypic frequency (GST) and similarity (RST), and no phylogeographic structure was detected among the populations. AFLP values also confirmed that the populations arenot very genetically differentiated. The principal component analysis based on pairwise genetic differences showed threegroupings without a geographical correlation. The AMOVA analysis indicates that theamount of variation is higher withinpopulations (82 %) than among populations (18 %). Results of the pollen flow/seed flow ratio indicated positive values foreach population, indicating that gene flow by seed is not more efficient than by pollen. Instead, the total pollen/seed flow for all population presents a negative value, suggesting that pollen dispersal does not appear to be more effective over thelong range for gene flow than seed dispersal. This differentiation level supports the hypothesis that the fragmentation and isolation of the residual populations is in progress. Thisphenomenon is due not only to post-ice age climate changes, but also to direct and indirect anthropic actions.

KW - AFLP

KW - Conservation

KW - CpSSR

KW - Genetic structure

KW - Paleoendemic

KW - Petagnaea gussonei

KW - Seed/pollen flow

KW - Sicily

UR - http://hdl.handle.net/10447/78797

M3 - Article

VL - 299

SP - 209

EP - 223

JO - Plant Systematics and Evolution

JF - Plant Systematics and Evolution

SN - 0378-2697

ER -