GENERATION OF PREVASCULARIZED PLLABIODEGRADABLE SCAFFOLDS BY DIPDRAWING AND DIFFUSION INDUCED PHASESEPARATION (DIPS).

Risultato della ricerca: Other

Abstract

A critical obstacle in tissue engineering is to develop amassive structure of living cells upon transfer from thein vitro culture conditions into the host in vivo. Avascular network is required to supply essentialnutrients, including oxygen, remove metabolic wasteproducts and provide a biochemical communication“highway”. For these reasons to build an implantablestructure in which vessel formation (angiogenesis) takeplace is mandatory. PLLA scaffolds usable in vasculartissue engineering were generated by dip-coating viaDiffusion Induced Phase Separation (DIPS) technique.The scaffolds, with a vessel-like shape, were obtainedby performing a DIPS process around a nylon fibrewhose diameter was 700 μm. The fibre was firstimmersed into a 4% PLLA dioxane solution andsubsequently immersed into a second bath containingdistilled water. The covered fibre was then washed inorder to eliminate the excess of dioxane and dried;finally the internal nylon fibre was pulled out so as toobtain a hollow biodegradable PLLA fibber. ByScanning Electron Microscopy observation the scaffoldshave a lumen of ca. 700 μm and the internal surfaceshow to be homogeneous with micro-pores of about 1-2μm diameter. Moreover, a cross section analysis showedan open structure across the thickness of the scaffoldwalls. Inside the generated scaffolds we have culturedhuman endothelial cells; these have shown to be able togrow within the scaffolds and after 3 weeks they beginto form a “primordial” vessel-like structure, showing awell differentiate epithelial tissue.
Lingua originaleEnglish
Pagine36-36
Numero di pagine1
Stato di pubblicazionePublished - 2009

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