Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.

Gergely Orban, András Czurkó, Péter Baracskay, Zsuzsanna Szepesi, Gábor Juhász, Gergely Orbán

    Risultato della ricerca: Article

    14 Citazioni (Scopus)

    Abstract

    Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of "dark" neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of "dark" neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.
    Lingua originaleEnglish
    pagine (da-a)217-228
    Numero di pagine12
    RivistaBrain Research
    Volume1228
    Stato di pubblicazionePublished - 2008

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    4-Aminopyridine
    Hippocampus
    Seizures
    Neurons
    Electroencephalography
    Epilepsy
    Occipital Lobe
    Halothane
    Silver
    Anesthesia
    Pontine Tegmentum

    All Science Journal Classification (ASJC) codes

    • Neuroscience(all)
    • Developmental Biology
    • Clinical Neurology
    • Molecular Biology

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    Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat. / Orban, Gergely; Czurkó, András; Baracskay, Péter; Szepesi, Zsuzsanna; Juhász, Gábor; Orbán, Gergely.

    In: Brain Research, Vol. 1228, 2008, pag. 217-228.

    Risultato della ricerca: Article

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    title = "Generalization of seizures parallels the formation of {"}dark{"} neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.",
    abstract = "Distribution and time course of the occurrence of {"}dark{"} neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of {"}dark{"} neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of {"}dark{"} neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.",
    keywords = "* Epilepsy; * Dark neuron; * Hippocampus; * Pontine reticular formation; * Cell injury; * Animal model; * Neurogliaform cell; * Astrocyte; * Status epilepticus",
    author = "Gergely Orban and Andr{\'a}s Czurk{\'o} and P{\'e}ter Baracskay and Zsuzsanna Szepesi and G{\'a}bor Juh{\'a}sz and Gergely Orb{\'a}n",
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    language = "English",
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    T1 - Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.

    AU - Orban, Gergely

    AU - Czurkó, András

    AU - Baracskay, Péter

    AU - Szepesi, Zsuzsanna

    AU - Juhász, Gábor

    AU - Orbán, Gergely

    PY - 2008

    Y1 - 2008

    N2 - Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of "dark" neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of "dark" neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.

    AB - Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of "dark" neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of "dark" neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.

    KW - Epilepsy; Dark neuron; Hippocampus; Pontine reticular formation; Cell injury; Animal model; Neurogliaform cell; Astrocyte; Status epilepticus

    UR - http://hdl.handle.net/10447/64201

    UR - http://www.sciencedirect.com/science/article/pii/S0006899308014807

    M3 - Article

    VL - 1228

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    EP - 228

    JO - Brain Research

    JF - Brain Research

    SN - 0006-8993

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