Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture

Valentina Bravata', Luigi Minafra, Maria Carla Gilardi, Valentina Bravata, Francesco Paolo Cammarata, Giusi Irma Forte, Cristina Messa

    Risultato della ricerca: Article

    18 Citazioni (Scopus)

    Abstract

    Background/Aim: Epithelial–mesenchymal transition(EMT) is a process co-opted by cancer cells to invade and formmetastases. In the present study we analyzed gene expressionprofiles of primary breast cancer cells in culture in order tohighlight genes related to EMT. Materials and Methods:Microarray expression analysis of primary cells isolated froma specimen of a patient with an infiltrating ductal carcinoma ofthe breast was performed. Real-Time Quantitative ReverseTranscription PCR (qRT-PCR) analyses validated microarraygene expression trends. Results: Thirty-six candidate genes wereselected and used to generate a molecular network displayingthe tight relationship among them. The most significant GeneOntology biological processes characterizing this network wereinvolved in cell migration and motility. Conclusion: Our datarevealed the involvement of new genes which displayed tightrelationships among them, suggesting a molecular network inwhich they could contribute to control of EMT in breast cancer.This study may offer a basis for understanding complexmechanisms which regulate breast cancer progression and fordesigning individualized anticancer therapies.
    Lingua originaleEnglish
    Numero di pagine11
    RivistaAnticancer Research
    Volume34(5)
    Stato di pubblicazionePublished - 2014

    Fingerprint

    Gene Expression Profiling
    Cell Culture Techniques
    Breast Neoplasms
    Cell Movement
    Genes
    Carcinoma, Ductal, Breast
    Biological Phenomena
    Gene Order
    Microarray Analysis
    Real-Time Polymerase Chain Reaction
    Neoplasms
    Therapeutics

    All Science Journal Classification (ASJC) codes

    • Oncology
    • Cancer Research

    Cita questo

    Bravata', V., Minafra, L., Gilardi, M. C., Bravata, V., Cammarata, F. P., Forte, G. I., & Messa, C. (2014). Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture. Anticancer Research, 34(5).

    Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture. / Bravata', Valentina; Minafra, Luigi; Gilardi, Maria Carla; Bravata, Valentina; Cammarata, Francesco Paolo; Forte, Giusi Irma; Messa, Cristina.

    In: Anticancer Research, Vol. 34(5), 2014.

    Risultato della ricerca: Article

    Bravata', V, Minafra, L, Gilardi, MC, Bravata, V, Cammarata, FP, Forte, GI & Messa, C 2014, 'Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture', Anticancer Research, vol. 34(5).
    Bravata', Valentina ; Minafra, Luigi ; Gilardi, Maria Carla ; Bravata, Valentina ; Cammarata, Francesco Paolo ; Forte, Giusi Irma ; Messa, Cristina. / Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture. In: Anticancer Research. 2014 ; Vol. 34(5).
    @article{ee08427744fd4270b4aaa77dd4e2f2a9,
    title = "Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture",
    abstract = "Background/Aim: Epithelial–mesenchymal transition(EMT) is a process co-opted by cancer cells to invade and formmetastases. In the present study we analyzed gene expressionprofiles of primary breast cancer cells in culture in order tohighlight genes related to EMT. Materials and Methods:Microarray expression analysis of primary cells isolated froma specimen of a patient with an infiltrating ductal carcinoma ofthe breast was performed. Real-Time Quantitative ReverseTranscription PCR (qRT-PCR) analyses validated microarraygene expression trends. Results: Thirty-six candidate genes wereselected and used to generate a molecular network displayingthe tight relationship among them. The most significant GeneOntology biological processes characterizing this network wereinvolved in cell migration and motility. Conclusion: Our datarevealed the involvement of new genes which displayed tightrelationships among them, suggesting a molecular network inwhich they could contribute to control of EMT in breast cancer.This study may offer a basis for understanding complexmechanisms which regulate breast cancer progression and fordesigning individualized anticancer therapies.",
    author = "Valentina Bravata' and Luigi Minafra and Gilardi, {Maria Carla} and Valentina Bravata and Cammarata, {Francesco Paolo} and Forte, {Giusi Irma} and Cristina Messa",
    year = "2014",
    language = "English",
    volume = "34(5)",
    journal = "Anticancer Research",
    issn = "0250-7005",
    publisher = "International Institute of Anticancer Research",

    }

    TY - JOUR

    T1 - Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture

    AU - Bravata', Valentina

    AU - Minafra, Luigi

    AU - Gilardi, Maria Carla

    AU - Bravata, Valentina

    AU - Cammarata, Francesco Paolo

    AU - Forte, Giusi Irma

    AU - Messa, Cristina

    PY - 2014

    Y1 - 2014

    N2 - Background/Aim: Epithelial–mesenchymal transition(EMT) is a process co-opted by cancer cells to invade and formmetastases. In the present study we analyzed gene expressionprofiles of primary breast cancer cells in culture in order tohighlight genes related to EMT. Materials and Methods:Microarray expression analysis of primary cells isolated froma specimen of a patient with an infiltrating ductal carcinoma ofthe breast was performed. Real-Time Quantitative ReverseTranscription PCR (qRT-PCR) analyses validated microarraygene expression trends. Results: Thirty-six candidate genes wereselected and used to generate a molecular network displayingthe tight relationship among them. The most significant GeneOntology biological processes characterizing this network wereinvolved in cell migration and motility. Conclusion: Our datarevealed the involvement of new genes which displayed tightrelationships among them, suggesting a molecular network inwhich they could contribute to control of EMT in breast cancer.This study may offer a basis for understanding complexmechanisms which regulate breast cancer progression and fordesigning individualized anticancer therapies.

    AB - Background/Aim: Epithelial–mesenchymal transition(EMT) is a process co-opted by cancer cells to invade and formmetastases. In the present study we analyzed gene expressionprofiles of primary breast cancer cells in culture in order tohighlight genes related to EMT. Materials and Methods:Microarray expression analysis of primary cells isolated froma specimen of a patient with an infiltrating ductal carcinoma ofthe breast was performed. Real-Time Quantitative ReverseTranscription PCR (qRT-PCR) analyses validated microarraygene expression trends. Results: Thirty-six candidate genes wereselected and used to generate a molecular network displayingthe tight relationship among them. The most significant GeneOntology biological processes characterizing this network wereinvolved in cell migration and motility. Conclusion: Our datarevealed the involvement of new genes which displayed tightrelationships among them, suggesting a molecular network inwhich they could contribute to control of EMT in breast cancer.This study may offer a basis for understanding complexmechanisms which regulate breast cancer progression and fordesigning individualized anticancer therapies.

    UR - http://hdl.handle.net/10447/100600

    UR - http://www.ncbi.nlm.nih.gov/pubmed/24778019

    M3 - Article

    VL - 34(5)

    JO - Anticancer Research

    JF - Anticancer Research

    SN - 0250-7005

    ER -