Background/Aim: Epithelial–mesenchymal transition(EMT) is a process co-opted by cancer cells to invade and formmetastases. In the present study we analyzed gene expressionprofiles of primary breast cancer cells in culture in order tohighlight genes related to EMT. Materials and Methods:Microarray expression analysis of primary cells isolated froma specimen of a patient with an infiltrating ductal carcinoma ofthe breast was performed. Real-Time Quantitative ReverseTranscription PCR (qRT-PCR) analyses validated microarraygene expression trends. Results: Thirty-six candidate genes wereselected and used to generate a molecular network displayingthe tight relationship among them. The most significant GeneOntology biological processes characterizing this network wereinvolved in cell migration and motility. Conclusion: Our datarevealed the involvement of new genes which displayed tightrelationships among them, suggesting a molecular network inwhich they could contribute to control of EMT in breast cancer.This study may offer a basis for understanding complexmechanisms which regulate breast cancer progression and fordesigning individualized anticancer therapies.
|Numero di pagine||11|
|Stato di pubblicazione||Published - 2014|
All Science Journal Classification (ASJC) codes
- Cancer Research
Bravata', V., Minafra, L., Gilardi, M. C., Bravata, V., Cammarata, F. P., Forte, G. I., & Messa, C. (2014). Gene Expression Profiling of Epithelial–Mesenchymal Transition in Primary Breast Cancer Cell Culture. Anticancer Research, 34(5).