Gene expression in mouse spermatogenesis during ontogenesis.

Maurizio Averna, Franca Maria Pezzino, Giorgio Nicotra, Laura Litrico, Paola Castrogiovanni, Fabrizio Romano, Enzo Vicari, Fabio D'Amico, Vincenza Giuffrida, Rosa Imbesi, Massimo Libra, Salvatore Travali, Rosario D'Agata, Maria Rita Garofalo, Aldo E. Calogero, Santo Sanfilippo, Maria Maddalena Garofalo, Salvatore Sanfilippo

Risultato della ricerca: Article

3 Citazioni (Scopus)

Abstract

In this study, we evaluated the expression of genes probably involved in spermatogenesis in the mouse. We examined cytosolic chaperonin theta subunit (CCTtheta), Ngg1 interacting factor 3 like 1 binding protein 1 (NIF3L1 BP1) and apolipoprotein H (ApoH) expression during mouse onto-geny using RT-PCR. Testicular tissue was obtained from mice 3, 6, 8, 10, 12, 14, 18, 20 and 40 (adult) days after birth. For each mouse, one testis was used for histological examination, whereas RNA was extracted from the controlateral testis for expression analysis. RT-PCR analysis showed that CCTtheta gene expression was low until day 10, but increased drastically afterwards. At this age, spermatocytes started to be present in the mouse testis. Therefore, CCT protein could be involved in chromatin packaging and remodeling during spermiogenesis, as also suggested by other studies. NIF3L1 BP1 expression increased steadily during ontogenesis reaching maximum levels in the adult mouse when all germ cell stages are present. This finding suggests that NIF3L1 BP1 is a gene not expressed by a specific germ cell type. ApoH expression was very low or absent during prepuberal stages, whereas it was detectable in the adult testis when spermatogenesis was completed. This suggests that ApoH may be involved in clearing apoptotic bodies during spermatogenesis since apoptotic events increase during spermatogenesis. This study contributes to understanding the role played by genes important for spermatogenesis.
Lingua originaleEnglish
pagine (da-a)523-528
Numero di pagine6
RivistaInternational Journal of Molecular Medicine
Volume17
Stato di pubblicazionePublished - 2006

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Spermatogenesis
beta 2-Glycoprotein I
Gene Expression
Testis
Carrier Proteins
Germ Cells
Chaperonin Containing TCP-1
Polymerase Chain Reaction
Chromatin Assembly and Disassembly
Spermatocytes
Product Packaging
Genes
Parturition
RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cita questo

Averna, M., Pezzino, F. M., Nicotra, G., Litrico, L., Castrogiovanni, P., Romano, F., ... Sanfilippo, S. (2006). Gene expression in mouse spermatogenesis during ontogenesis. International Journal of Molecular Medicine, 17, 523-528.

Gene expression in mouse spermatogenesis during ontogenesis. / Averna, Maurizio; Pezzino, Franca Maria; Nicotra, Giorgio; Litrico, Laura; Castrogiovanni, Paola; Romano, Fabrizio; Vicari, Enzo; D'Amico, Fabio; Giuffrida, Vincenza; Imbesi, Rosa; Libra, Massimo; Travali, Salvatore; D'Agata, Rosario; Garofalo, Maria Rita; Calogero, Aldo E.; Sanfilippo, Santo; Garofalo, Maria Maddalena; Sanfilippo, Salvatore.

In: International Journal of Molecular Medicine, Vol. 17, 2006, pag. 523-528.

Risultato della ricerca: Article

Averna, M, Pezzino, FM, Nicotra, G, Litrico, L, Castrogiovanni, P, Romano, F, Vicari, E, D'Amico, F, Giuffrida, V, Imbesi, R, Libra, M, Travali, S, D'Agata, R, Garofalo, MR, Calogero, AE, Sanfilippo, S, Garofalo, MM & Sanfilippo, S 2006, 'Gene expression in mouse spermatogenesis during ontogenesis.', International Journal of Molecular Medicine, vol. 17, pagg. 523-528.
Averna M, Pezzino FM, Nicotra G, Litrico L, Castrogiovanni P, Romano F e altri. Gene expression in mouse spermatogenesis during ontogenesis. International Journal of Molecular Medicine. 2006;17:523-528.
Averna, Maurizio ; Pezzino, Franca Maria ; Nicotra, Giorgio ; Litrico, Laura ; Castrogiovanni, Paola ; Romano, Fabrizio ; Vicari, Enzo ; D'Amico, Fabio ; Giuffrida, Vincenza ; Imbesi, Rosa ; Libra, Massimo ; Travali, Salvatore ; D'Agata, Rosario ; Garofalo, Maria Rita ; Calogero, Aldo E. ; Sanfilippo, Santo ; Garofalo, Maria Maddalena ; Sanfilippo, Salvatore. / Gene expression in mouse spermatogenesis during ontogenesis. In: International Journal of Molecular Medicine. 2006 ; Vol. 17. pagg. 523-528.
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title = "Gene expression in mouse spermatogenesis during ontogenesis.",
abstract = "In this study, we evaluated the expression of genes probably involved in spermatogenesis in the mouse. We examined cytosolic chaperonin theta subunit (CCTtheta), Ngg1 interacting factor 3 like 1 binding protein 1 (NIF3L1 BP1) and apolipoprotein H (ApoH) expression during mouse onto-geny using RT-PCR. Testicular tissue was obtained from mice 3, 6, 8, 10, 12, 14, 18, 20 and 40 (adult) days after birth. For each mouse, one testis was used for histological examination, whereas RNA was extracted from the controlateral testis for expression analysis. RT-PCR analysis showed that CCTtheta gene expression was low until day 10, but increased drastically afterwards. At this age, spermatocytes started to be present in the mouse testis. Therefore, CCT protein could be involved in chromatin packaging and remodeling during spermiogenesis, as also suggested by other studies. NIF3L1 BP1 expression increased steadily during ontogenesis reaching maximum levels in the adult mouse when all germ cell stages are present. This finding suggests that NIF3L1 BP1 is a gene not expressed by a specific germ cell type. ApoH expression was very low or absent during prepuberal stages, whereas it was detectable in the adult testis when spermatogenesis was completed. This suggests that ApoH may be involved in clearing apoptotic bodies during spermatogenesis since apoptotic events increase during spermatogenesis. This study contributes to understanding the role played by genes important for spermatogenesis.",
author = "Maurizio Averna and Pezzino, {Franca Maria} and Giorgio Nicotra and Laura Litrico and Paola Castrogiovanni and Fabrizio Romano and Enzo Vicari and Fabio D'Amico and Vincenza Giuffrida and Rosa Imbesi and Massimo Libra and Salvatore Travali and Rosario D'Agata and Garofalo, {Maria Rita} and Calogero, {Aldo E.} and Santo Sanfilippo and Garofalo, {Maria Maddalena} and Salvatore Sanfilippo",
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AU - Averna, Maurizio

AU - Pezzino, Franca Maria

AU - Nicotra, Giorgio

AU - Litrico, Laura

AU - Castrogiovanni, Paola

AU - Romano, Fabrizio

AU - Vicari, Enzo

AU - D'Amico, Fabio

AU - Giuffrida, Vincenza

AU - Imbesi, Rosa

AU - Libra, Massimo

AU - Travali, Salvatore

AU - D'Agata, Rosario

AU - Garofalo, Maria Rita

AU - Calogero, Aldo E.

AU - Sanfilippo, Santo

AU - Garofalo, Maria Maddalena

AU - Sanfilippo, Salvatore

PY - 2006

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N2 - In this study, we evaluated the expression of genes probably involved in spermatogenesis in the mouse. We examined cytosolic chaperonin theta subunit (CCTtheta), Ngg1 interacting factor 3 like 1 binding protein 1 (NIF3L1 BP1) and apolipoprotein H (ApoH) expression during mouse onto-geny using RT-PCR. Testicular tissue was obtained from mice 3, 6, 8, 10, 12, 14, 18, 20 and 40 (adult) days after birth. For each mouse, one testis was used for histological examination, whereas RNA was extracted from the controlateral testis for expression analysis. RT-PCR analysis showed that CCTtheta gene expression was low until day 10, but increased drastically afterwards. At this age, spermatocytes started to be present in the mouse testis. Therefore, CCT protein could be involved in chromatin packaging and remodeling during spermiogenesis, as also suggested by other studies. NIF3L1 BP1 expression increased steadily during ontogenesis reaching maximum levels in the adult mouse when all germ cell stages are present. This finding suggests that NIF3L1 BP1 is a gene not expressed by a specific germ cell type. ApoH expression was very low or absent during prepuberal stages, whereas it was detectable in the adult testis when spermatogenesis was completed. This suggests that ApoH may be involved in clearing apoptotic bodies during spermatogenesis since apoptotic events increase during spermatogenesis. This study contributes to understanding the role played by genes important for spermatogenesis.

AB - In this study, we evaluated the expression of genes probably involved in spermatogenesis in the mouse. We examined cytosolic chaperonin theta subunit (CCTtheta), Ngg1 interacting factor 3 like 1 binding protein 1 (NIF3L1 BP1) and apolipoprotein H (ApoH) expression during mouse onto-geny using RT-PCR. Testicular tissue was obtained from mice 3, 6, 8, 10, 12, 14, 18, 20 and 40 (adult) days after birth. For each mouse, one testis was used for histological examination, whereas RNA was extracted from the controlateral testis for expression analysis. RT-PCR analysis showed that CCTtheta gene expression was low until day 10, but increased drastically afterwards. At this age, spermatocytes started to be present in the mouse testis. Therefore, CCT protein could be involved in chromatin packaging and remodeling during spermiogenesis, as also suggested by other studies. NIF3L1 BP1 expression increased steadily during ontogenesis reaching maximum levels in the adult mouse when all germ cell stages are present. This finding suggests that NIF3L1 BP1 is a gene not expressed by a specific germ cell type. ApoH expression was very low or absent during prepuberal stages, whereas it was detectable in the adult testis when spermatogenesis was completed. This suggests that ApoH may be involved in clearing apoptotic bodies during spermatogenesis since apoptotic events increase during spermatogenesis. This study contributes to understanding the role played by genes important for spermatogenesis.

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