Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | 113-122 |
| Numero di pagine | 10 |
| Rivista | CITRUS RESEARCH & TECHNOLOGY |
| Volume | 37 |
| Stato di pubblicazione | Published - 2016 |
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Gametic embryogenesis through isolated microspore culture in mandarin (Citrus reticulata Blanco), Mandarino Tardivo Di Ciaculli: effect of meta-Topolin and temperature treatments. / Cimò, G.; Casamento, D.; Torello Marinoni, D.; Botta, R.; Germanà, M.
In: CITRUS RESEARCH & TECHNOLOGY, Vol. 37, 2016, pag. 113-122.Risultato della ricerca: Article
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TY - JOUR
T1 - Gametic embryogenesis through isolated microspore culture in mandarin (Citrus reticulata Blanco), Mandarino Tardivo Di Ciaculli: effect of meta-Topolin and temperature treatments
AU - Cimò, G.; Casamento, D.; Torello Marinoni, D.; Botta, R.; Germanà, M.
AU - Germana', Maria
AU - Cimo', Giuseppe
PY - 2016
Y1 - 2016
N2 - Haploid technology, allowing the single-step development of complete homozygous genotypes from heterozygous plants through gametic embryogenesis, has already an enormous impact on breeding programs of many important crops. Microspore embryogenesis can be carried out through in vitro culture of anther or isolated microspore. Experiments have been carried out via isolated microspore culture in Citrus reticulata (Blanco), Mandarino Tardivo di Ciaculli, investigating the influence of two culture media, of two plant growth regulator types and of two temperature treatments applied before the culture or just after the culture. After ten months of culture, for all the media tested, different structural features have been observed and registered: uninucleated, binucleated, multinucleated microspores, and, for the first time in mandarin isolated culture, calli and microspore-derived embryos. These results represent advancement in the knowledge of microspore embryogenesis in mandarin. Actually, this is the first time that embryo regeneration from isolated microspore cultures has been reported in mandarin, a genotype very recalcitrant to microspore embryogenesis
AB - Haploid technology, allowing the single-step development of complete homozygous genotypes from heterozygous plants through gametic embryogenesis, has already an enormous impact on breeding programs of many important crops. Microspore embryogenesis can be carried out through in vitro culture of anther or isolated microspore. Experiments have been carried out via isolated microspore culture in Citrus reticulata (Blanco), Mandarino Tardivo di Ciaculli, investigating the influence of two culture media, of two plant growth regulator types and of two temperature treatments applied before the culture or just after the culture. After ten months of culture, for all the media tested, different structural features have been observed and registered: uninucleated, binucleated, multinucleated microspores, and, for the first time in mandarin isolated culture, calli and microspore-derived embryos. These results represent advancement in the knowledge of microspore embryogenesis in mandarin. Actually, this is the first time that embryo regeneration from isolated microspore cultures has been reported in mandarin, a genotype very recalcitrant to microspore embryogenesis
UR - http://hdl.handle.net/10447/279184
M3 - Article
VL - 37
SP - 113
EP - 122
JO - CITRUS RESEARCH & TECHNOLOGY
JF - CITRUS RESEARCH & TECHNOLOGY
SN - 2177-5419
ER -