Gametic embryogenesis through isolated microspore culture in Corylus avellana L

Maria Germana', Valeria Gianguzzi, Benedetta Chiancone, Roberto Botta, Chiara Sartor

Risultato della ricerca: Article

7 Citazioni (Scopus)

Abstract

Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report of early embryos being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryos had a single allele per each locus whereas the parent cultivar was heterozygous, indicating they developed from haploid microspores. The response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis in other fruit crops.
Lingua originaleEnglish
pagine (da-a)635-647
Numero di pagine13
RivistaPLANT CELL TISSUE AND ORGAN CULTURE
Volume124
Stato di pubblicazionePublished - 2016

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Corylus avellana
microspores
embryogenesis
embryo (plant)
genotype
hazelnuts
haploidy
nut trees
fruit crops
self-pollination
homozygosity
plant breeding
woody plants
germ cells
production technology
culture media
developmental stages
alleles
loci
cultivars

All Science Journal Classification (ASJC) codes

  • Horticulture

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Gametic embryogenesis through isolated microspore culture in Corylus avellana L. / Germana', Maria; Gianguzzi, Valeria; Chiancone, Benedetta; Botta, Roberto; Sartor, Chiara.

In: PLANT CELL TISSUE AND ORGAN CULTURE, Vol. 124, 2016, pag. 635-647.

Risultato della ricerca: Article

Germana', Maria ; Gianguzzi, Valeria ; Chiancone, Benedetta ; Botta, Roberto ; Sartor, Chiara. / Gametic embryogenesis through isolated microspore culture in Corylus avellana L. In: PLANT CELL TISSUE AND ORGAN CULTURE. 2016 ; Vol. 124. pagg. 635-647.
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abstract = "Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report of early embryos being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryos had a single allele per each locus whereas the parent cultivar was heterozygous, indicating they developed from haploid microspores. The response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis in other fruit crops.",
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AU - Germana', Maria

AU - Gianguzzi, Valeria

AU - Chiancone, Benedetta

AU - Botta, Roberto

AU - Sartor, Chiara

PY - 2016

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AB - Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report of early embryos being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryos had a single allele per each locus whereas the parent cultivar was heterozygous, indicating they developed from haploid microspores. The response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis in other fruit crops.

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