G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes

Annamaria Martorana, Angelo Spinello, Giampaolo Barone, Alessio Terenzi, Riccardo Bonsignore, Anna Maria Almerico, Antonino Lauria, Alessio Terenzi, Bernhard K. Keppler, Annamaria Martorana, Angelo Spinello, Riccardo Bonsignore, Giampaolo Barone, Antonino Lauria, Anna Maria Almerico

Risultato della ricerca: Articlepeer review

27 Citazioni (Scopus)

Abstract

Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N′ bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, Kb, 2.6 × 105 M- 1 and 3.5 × 104 M- 1, respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation.
Lingua originaleEnglish
pagine (da-a)115-121
Numero di pagine7
RivistaJournal of Inorganic Biochemistry
Volume161
Stato di pubblicazionePublished - 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Inorganic Chemistry

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