TY - JOUR
T1 - G-quadruplex vs. duplex-DNA binding of nickel(II) and zinc(II) Schiff base complexes
AU - Martorana, Annamaria
AU - Barone, Giampaolo
AU - Spinello, Angelo
AU - Terenzi, Alessio
AU - Bonsignore, Riccardo
AU - Lauria, Antonino
AU - Almerico, Anna Maria
AU - Terenzi, Alessio
AU - Keppler, Bernhard K.
AU - Martorana, Annamaria
AU - Spinello, Angelo
AU - Bonsignore, Riccardo
AU - Barone, Giampaolo
AU - Lauria, Antonino
AU - Almerico, Anna Maria
PY - 2016
Y1 - 2016
N2 - Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N′ bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, Kb, 2.6 × 105 M- 1 and 3.5 × 104 M- 1, respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation.
AB - Novel nickel(II) (1) and zinc(II) (2) complexes of a Salen-like ligand, carrying a pyrimidine ring on the N,N′ bridge, were synthesized and characterized. Their interaction with duplex and G-quadruplex DNA was investigated in aqueous solution through UV-visible absorption, circular dichroism and viscometry measurements. The results obtained point out that, while the zinc(II) complex does not interact with both duplex and G-quadruplex DNA, the nickel(II) complex 1 binds preferentially to G-quadruplex respect to duplex-DNA, with values of the DNA-binding constants, Kb, 2.6 × 105 M- 1 and 3.5 × 104 M- 1, respectively. Molecular dynamics simulations provided an atomic level model of the top-stacking binding occurring between 1 and hTelo (a 22-mer sequence oligonucleotide) in G-quadruplex conformation.
UR - http://hdl.handle.net/10447/214676
M3 - Article
SN - 0162-0134
VL - 161
SP - 115
EP - 121
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
ER -