Functional and dysfunctional conformers of human neuroserpin Characterized by optical spectroscopies and molecular dynamics

Antonio Cupane, Maria Grazia Santangelo, Matteo Levantino, Mauro Manno, Martino Bolognesi, Daniele Parisi, Stefano Ricagno, Maria Rosalia Mangione, Vincenzo Martorana, Rosina Noto, Maria Grazia Santangelo

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

Neuroserpin (NS) is a serine protease inhibitor (SERPIN) involved in different neurological pathologies, including the Familial Encephalopathy with Neuroserpin Inclusion Bodies (FENIB), related to the aberrant polymerization of NS mutants. Here we present an in vitro and in silico characterization of native neuroserpin and its dysfunctional conformation isoforms: the proteolytically cleaved conformer, the inactive latent conformer, and the polymeric species. Based on circular dichroism and fluorescence spectroscopy, we present an experimental validation of the latent model and highlight the main structural features of the different conformers. In particular, emission spectra of aromatic residues yield distinct conformational fingerprints, that provide a novel and simple spectroscopic tool for selecting serpin conformers in vitro. Based on the structural relationship between cleaved and latent serpins, we propose a structural model for latent NS, for which an experimental crystallographic structure is lacking. Molecular Dynamics simulations suggest that NS conformational stability and flexibility arise from a spatial distribution of intramolecular salt-bridges and hydrogen bonds.
Lingua originaleEnglish
pagine (da-a)110-117
Numero di pagine8
RivistaBIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume1854
Stato di pubblicazionePublished - 2015

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All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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