From microbial proteomics to synthetic biology: Amycolatopsis balhimycina case

Our investigation, performed by combining data from differentialproteomic analyses carried-out using wild-type (Wt) and non-producing strains incubated in differentgrowth conditions, showed that antibiotic production is always associated with the up-regulation ofeither specific enzymes of balhimycin (bal) biosynthetic gene cluster and enzymes related to centralcarbon metabolism, cell energy and redox balance. Thus this approach suggested new insights toimprove fermentation technology strategies and revealed target genes for synthetic biology approachesaimed to improve antibiotic yield production.