Fragments of -thymosin from the sea urchin Paracentrotus lividus as potential antimicrobial peptides against staphylococcal biofilms

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Abstract

The immune mediators in echinoderms can be a potential source of novel antimicrobial peptides (AMPs) applied toward controlling pathogenic staphylococcal biofilms that are intrinsically resistant to conventional antibiotics. The peptide fraction <5 kDa from the cytosol of coelomocytes of the sea urchin Paracentrotus lividus (5-CC) was tested against a group of Gram-positive and Gram-negative pathogen reference strains. The 5-CC of P. lividus was active against all planktonic-tested strains but also showed antibiofilm properties against staphylococcal strains. Additionally,wedemonstrated the presenceof three smallpeptides in the5-CCbelonging tosegment 9-41of aP. lividus -thymosin. The smallest of these peptides in particular, showed the common chemical–physical characteristics of AMPs. This novel AMP from -thymosin has high potential activity as an antibiofilm agent, acting on slow-growing bacterial cells that exhibit a reduced susceptibility to conventional antibiotics and represent a reservoir for recurrent biofilm-associated infections.
Lingua originaleEnglish
pagine (da-a)79-85
Numero di pagine7
RivistaAnnals of the New York Academy of Sciences
Volume1270
Stato di pubblicazionePublished - 2012

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Thymosin
Biofilms
Oceans and Seas
Peptides
Paracentrotus
Anti-Bacterial Agents
Sea Urchins
Pathogens
Cytosol
Antibiotics
Conventional
Biofilm
Infection
Physical
Susceptibility
Mediator
Cells

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • History and Philosophy of Science
  • Biochemistry, Genetics and Molecular Biology(all)

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title = "Fragments of -thymosin from the sea urchin Paracentrotus lividus as potential antimicrobial peptides against staphylococcal biofilms",
abstract = "The immune mediators in echinoderms can be a potential source of novel antimicrobial peptides (AMPs) applied toward controlling pathogenic staphylococcal biofilms that are intrinsically resistant to conventional antibiotics. The peptide fraction <5 kDa from the cytosol of coelomocytes of the sea urchin Paracentrotus lividus (5-CC) was tested against a group of Gram-positive and Gram-negative pathogen reference strains. The 5-CC of P. lividus was active against all planktonic-tested strains but also showed antibiofilm properties against staphylococcal strains. Additionally,wedemonstrated the presenceof three smallpeptides in the5-CCbelonging tosegment 9-41of aP. lividus -thymosin. The smallest of these peptides in particular, showed the common chemical–physical characteristics of AMPs. This novel AMP from -thymosin has high potential activity as an antibiofilm agent, acting on slow-growing bacterial cells that exhibit a reduced susceptibility to conventional antibiotics and represent a reservoir for recurrent biofilm-associated infections.",
keywords = "antibiofilm agents; antimicrobial peptides (AMPs); staphylococcal biofilms",
author = "Domenico Schillaci and Vincenzo Arizza and Cusimano, {Maria Grazia} and Maria Vitale",
year = "2012",
language = "English",
volume = "1270",
pages = "79--85",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Fragments of -thymosin from the sea urchin Paracentrotus lividus as potential antimicrobial peptides against staphylococcal biofilms

AU - Schillaci, Domenico

AU - Arizza, Vincenzo

AU - Cusimano, Maria Grazia

AU - Vitale, Maria

PY - 2012

Y1 - 2012

N2 - The immune mediators in echinoderms can be a potential source of novel antimicrobial peptides (AMPs) applied toward controlling pathogenic staphylococcal biofilms that are intrinsically resistant to conventional antibiotics. The peptide fraction <5 kDa from the cytosol of coelomocytes of the sea urchin Paracentrotus lividus (5-CC) was tested against a group of Gram-positive and Gram-negative pathogen reference strains. The 5-CC of P. lividus was active against all planktonic-tested strains but also showed antibiofilm properties against staphylococcal strains. Additionally,wedemonstrated the presenceof three smallpeptides in the5-CCbelonging tosegment 9-41of aP. lividus -thymosin. The smallest of these peptides in particular, showed the common chemical–physical characteristics of AMPs. This novel AMP from -thymosin has high potential activity as an antibiofilm agent, acting on slow-growing bacterial cells that exhibit a reduced susceptibility to conventional antibiotics and represent a reservoir for recurrent biofilm-associated infections.

AB - The immune mediators in echinoderms can be a potential source of novel antimicrobial peptides (AMPs) applied toward controlling pathogenic staphylococcal biofilms that are intrinsically resistant to conventional antibiotics. The peptide fraction <5 kDa from the cytosol of coelomocytes of the sea urchin Paracentrotus lividus (5-CC) was tested against a group of Gram-positive and Gram-negative pathogen reference strains. The 5-CC of P. lividus was active against all planktonic-tested strains but also showed antibiofilm properties against staphylococcal strains. Additionally,wedemonstrated the presenceof three smallpeptides in the5-CCbelonging tosegment 9-41of aP. lividus -thymosin. The smallest of these peptides in particular, showed the common chemical–physical characteristics of AMPs. This novel AMP from -thymosin has high potential activity as an antibiofilm agent, acting on slow-growing bacterial cells that exhibit a reduced susceptibility to conventional antibiotics and represent a reservoir for recurrent biofilm-associated infections.

KW - antibiofilm agents; antimicrobial peptides (AMPs); staphylococcal biofilms

UR - http://hdl.handle.net/10447/78373

M3 - Article

VL - 1270

SP - 79

EP - 85

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -