The immune mediators in echinoderms can be a potential source of novel antimicrobial peptides (AMPs) appliedtoward controlling pathogenic staphylococcal biofilms that are intrinsically resistant to conventional antibiotics.The peptide fraction <5 kDa from the cytosol of coelomocytes of the sea urchin Paracentrotus lividus (5-CC)was tested against a group of Gram-positive and Gram-negative pathogen reference strains. The 5-CC of P. lividuswas active against all planktonic-tested strains but also showed antibiofilm properties against staphylococcal strains.Additionally,wedemonstrated the presenceof three smallpeptides in the5-CCbelonging tosegment 9-41of aP. lividus -thymosin. The smallest of these peptides in particular, showed the common chemical–physical characteristics ofAMPs. This novel AMP from -thymosin has high potential activity as an antibiofilm agent, acting on slow-growingbacterial cells that exhibit a reduced susceptibility to conventional antibiotics and represent a reservoir for recurrentbiofilm-associated infections.
|Numero di pagine||7|
|Rivista||Annals of the New York Academy of Sciences|
|Stato di pubblicazione||Published - 2012|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- History and Philosophy of Science