First report on the presence of Alloxan in bleached flour by LC-MS/MS method

Vita Di Stefano, Vincenzo Ferrantelli, Andrea Pulvirenti, Andrea Macaluso, Gaetano Cammilleri, Antonio Vella, Gianluigi Maria Lo Dico, Vita Giaccone, Rosa Pitonzo

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

In this work the presence of Alloxan in bread, pastry and cake bleached flour was investigate in order to verify possible risk for consumers related to the use of chemicals for flour bleaching. A selective UHPLC-MS/MS method has been developed and validated for the purpose. Alloxan is one of the possible minor side products of oxidation after chemical bleaching of wheat flours, when several chemical agents are used. One hundred and seventy-five flour samples were analyzed for Alloxan determination. The validation of the method was performed in accordance with the ISO/IEC/EN 17025 for linearity, detection limit, quantification limit, accuracy, precision and ruggedness determination. Satisfactory performances were obtained for the analyte, with a Limit of Detection (LOD) of 0.73 mg kg(-1), a Limit of Quantification (LOQ) of 0.85 mg kg(-1) and recovery values between 94% and 102%. The present work report for the first time the presence of trace levels of Alloxan in 24% of the analyzed samples, with mean values of 0,95 +/- 0,04 mg kg(-1). The presence of Alloxan was detected only in cake flour samples. Further studies on toxicological levels of Alloxan are needed in order to evaluate possible risks for consumers linked to the consumption of bakery products
Lingua originaleEnglish
pagine (da-a)120-125
Numero di pagine6
RivistaDefault journal
Volume77
Stato di pubblicazionePublished - 2017

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alloxan
Alloxan
Flour
flour
Bleaching
bleaching
Limit of Detection
Bakery products
detection limit
methodology
pastries
baked goods
Bread
wheat flour
sampling
Toxicology
breads
Triticum
tandem mass spectrometry
oxidation

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biochemistry

Cita questo

Di Stefano, V., Ferrantelli, V., Pulvirenti, A., Macaluso, A., Cammilleri, G., Vella, A., ... Pitonzo, R. (2017). First report on the presence of Alloxan in bleached flour by LC-MS/MS method. Default journal, 77, 120-125.

First report on the presence of Alloxan in bleached flour by LC-MS/MS method. / Di Stefano, Vita; Ferrantelli, Vincenzo; Pulvirenti, Andrea; Macaluso, Andrea; Cammilleri, Gaetano; Vella, Antonio; Lo Dico, Gianluigi Maria; Giaccone, Vita; Pitonzo, Rosa.

In: Default journal, Vol. 77, 2017, pag. 120-125.

Risultato della ricerca: Article

Di Stefano, V, Ferrantelli, V, Pulvirenti, A, Macaluso, A, Cammilleri, G, Vella, A, Lo Dico, GM, Giaccone, V & Pitonzo, R 2017, 'First report on the presence of Alloxan in bleached flour by LC-MS/MS method', Default journal, vol. 77, pagg. 120-125.
Di Stefano V, Ferrantelli V, Pulvirenti A, Macaluso A, Cammilleri G, Vella A e altri. First report on the presence of Alloxan in bleached flour by LC-MS/MS method. Default journal. 2017;77:120-125.
Di Stefano, Vita ; Ferrantelli, Vincenzo ; Pulvirenti, Andrea ; Macaluso, Andrea ; Cammilleri, Gaetano ; Vella, Antonio ; Lo Dico, Gianluigi Maria ; Giaccone, Vita ; Pitonzo, Rosa. / First report on the presence of Alloxan in bleached flour by LC-MS/MS method. In: Default journal. 2017 ; Vol. 77. pagg. 120-125.
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abstract = "In this work the presence of Alloxan in bread, pastry and cake bleached flour was investigate in order to verify possible risk for consumers related to the use of chemicals for flour bleaching. A selective UHPLC-MS/MS method has been developed and validated for the purpose. Alloxan is one of the possible minor side products of oxidation after chemical bleaching of wheat flours, when several chemical agents are used. One hundred and seventy-five flour samples were analyzed for Alloxan determination. The validation of the method was performed in accordance with the ISO/IEC/EN 17025 for linearity, detection limit, quantification limit, accuracy, precision and ruggedness determination. Satisfactory performances were obtained for the analyte, with a Limit of Detection (LOD) of 0.73 mg kg(-1), a Limit of Quantification (LOQ) of 0.85 mg kg(-1) and recovery values between 94{\%} and 102{\%}. The present work report for the first time the presence of trace levels of Alloxan in 24{\%} of the analyzed samples, with mean values of 0,95 +/- 0,04 mg kg(-1). The presence of Alloxan was detected only in cake flour samples. Further studies on toxicological levels of Alloxan are needed in order to evaluate possible risks for consumers linked to the consumption of bakery products",
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AU - Ferrantelli, Vincenzo

AU - Pulvirenti, Andrea

AU - Macaluso, Andrea

AU - Cammilleri, Gaetano

AU - Vella, Antonio

AU - Lo Dico, Gianluigi Maria

AU - Giaccone, Vita

AU - Pitonzo, Rosa

PY - 2017

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N2 - In this work the presence of Alloxan in bread, pastry and cake bleached flour was investigate in order to verify possible risk for consumers related to the use of chemicals for flour bleaching. A selective UHPLC-MS/MS method has been developed and validated for the purpose. Alloxan is one of the possible minor side products of oxidation after chemical bleaching of wheat flours, when several chemical agents are used. One hundred and seventy-five flour samples were analyzed for Alloxan determination. The validation of the method was performed in accordance with the ISO/IEC/EN 17025 for linearity, detection limit, quantification limit, accuracy, precision and ruggedness determination. Satisfactory performances were obtained for the analyte, with a Limit of Detection (LOD) of 0.73 mg kg(-1), a Limit of Quantification (LOQ) of 0.85 mg kg(-1) and recovery values between 94% and 102%. The present work report for the first time the presence of trace levels of Alloxan in 24% of the analyzed samples, with mean values of 0,95 +/- 0,04 mg kg(-1). The presence of Alloxan was detected only in cake flour samples. Further studies on toxicological levels of Alloxan are needed in order to evaluate possible risks for consumers linked to the consumption of bakery products

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