We investigated the antibacterial activity of Anemonia sulcata (Cnidaria, Anthozoa) tentacle andbody acidic extracts. Biochemical purification consisted of first step on solid phase Sep-Pak C8 columnfollowed by several HPLC runs on C18 column using different conditions. Anti-Micrococcuslysodeikticus activity has been detected in 40 % acetonitrile fractions. The resulting purified moleculefrom tentacles had a molecular mass determined by MALDI-TOF mass spectrum of 4946,299 Da and has been completely sequenced. Its aa sequence revealed identity with the Neurotoxin 2 (ATX-II), a Na+ channel blocking toxins. Consequently, ATX-II appeared to display a dual role as toxin and asantibacterial.
|Numero di pagine||10|
|Rivista||Invertebrate Survival Journal|
|Stato di pubblicazione||Published - 2014|
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