Feasibility of shotgun urinary proteomics for investigating prematurely born preschoolers (PBP)

Mauri, P.; Rossi, R.; De Palma, A.

Risultato della ricerca: Paper

Abstract

Background: Preterms and twins are at higher risk of respiratory morbidity later in life. Advances in proteomic approaches may allow the characterization of biomarkers involved in respiratory diseases (Mauri et al. Imm. Lett. 2014;162:2-10). Gel-free approach quantitatively identify differentially expressed proteins in relation to physiopathological conditions (Mauri&Dehò, Meth Enzymology 2008;447:99-117). This can improve the clinical reliability of the next generation of biomarkers to discriminate multiple phenotypes of childhood respiratory diseases. Aim: To assess the ability of gel-free proteomics for identifying specific protein profiles related to PBP. Methods: Urine samples were collected from 3-5 years children: 43 prematurely born (33.7 mean gestational age - GA), including 20 twins (34.4 mean GA), and 23 full-term born children, enrolled in the Preterm Asthma and Rhinitis (PRE-AR) ongoing longitudinal study at IBIM. An innovative approach based on nanochip liquid chromatography combined to high resolution mass spectrometry was used to obtain the proteomics profiles. Results: Proteomics analyses permitted the identification of 300 proteins in each urine sample, with a good repeatability (R2>0.99) and these are used for grouping the samples in relation to the different disease phenotypes. Conclusions: Obtained data indicate the proteomics approach as a promising platform for characterizing specific profiles related to PBP. These results represent a proof of principle for the proteomics application to all PRE-AR cases: along with clinical information, FeNO and lung function tests, this approach will attempt to discover candidate biomarkers and related metabolic pathways.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2016

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Firearms
Proteomics
Biomarkers
Rhinitis
Asthma
Gels
Urine
Phenotype
Proteins
Respiratory Function Tests
Metabolic Networks and Pathways
Liquid Chromatography
Gestational Age
Longitudinal Studies
Mass Spectrometry
Morbidity

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Feasibility of shotgun urinary proteomics for investigating prematurely born preschoolers (PBP). / Mauri, P.; Rossi, R.; De Palma, A.

2016.

Risultato della ricerca: Paper

@conference{47dc3b8c19d04c4e813b988ff3273e54,
title = "Feasibility of shotgun urinary proteomics for investigating prematurely born preschoolers (PBP)",
abstract = "Background: Preterms and twins are at higher risk of respiratory morbidity later in life. Advances in proteomic approaches may allow the characterization of biomarkers involved in respiratory diseases (Mauri et al. Imm. Lett. 2014;162:2-10). Gel-free approach quantitatively identify differentially expressed proteins in relation to physiopathological conditions (Mauri&Deh{\`o}, Meth Enzymology 2008;447:99-117). This can improve the clinical reliability of the next generation of biomarkers to discriminate multiple phenotypes of childhood respiratory diseases. Aim: To assess the ability of gel-free proteomics for identifying specific protein profiles related to PBP. Methods: Urine samples were collected from 3-5 years children: 43 prematurely born (33.7 mean gestational age - GA), including 20 twins (34.4 mean GA), and 23 full-term born children, enrolled in the Preterm Asthma and Rhinitis (PRE-AR) ongoing longitudinal study at IBIM. An innovative approach based on nanochip liquid chromatography combined to high resolution mass spectrometry was used to obtain the proteomics profiles. Results: Proteomics analyses permitted the identification of 300 proteins in each urine sample, with a good repeatability (R2>0.99) and these are used for grouping the samples in relation to the different disease phenotypes. Conclusions: Obtained data indicate the proteomics approach as a promising platform for characterizing specific profiles related to PBP. These results represent a proof of principle for the proteomics application to all PRE-AR cases: along with clinical information, FeNO and lung function tests, this approach will attempt to discover candidate biomarkers and related metabolic pathways.",
keywords = "Preterm, children",
author = "{Mauri, P.; Rossi, R.; De Palma, A.} and Giovanna Cilluffo and Giuliana Ferrante and Velia Malizia and {La Grutta}, Stefania and Sabrina D'Arpa and Gagliardo, {Rosalia Paola}",
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T1 - Feasibility of shotgun urinary proteomics for investigating prematurely born preschoolers (PBP)

AU - Mauri, P.; Rossi, R.; De Palma, A.

AU - Cilluffo, Giovanna

AU - Ferrante, Giuliana

AU - Malizia, Velia

AU - La Grutta, Stefania

AU - D'Arpa, Sabrina

AU - Gagliardo, Rosalia Paola

PY - 2016

Y1 - 2016

N2 - Background: Preterms and twins are at higher risk of respiratory morbidity later in life. Advances in proteomic approaches may allow the characterization of biomarkers involved in respiratory diseases (Mauri et al. Imm. Lett. 2014;162:2-10). Gel-free approach quantitatively identify differentially expressed proteins in relation to physiopathological conditions (Mauri&Dehò, Meth Enzymology 2008;447:99-117). This can improve the clinical reliability of the next generation of biomarkers to discriminate multiple phenotypes of childhood respiratory diseases. Aim: To assess the ability of gel-free proteomics for identifying specific protein profiles related to PBP. Methods: Urine samples were collected from 3-5 years children: 43 prematurely born (33.7 mean gestational age - GA), including 20 twins (34.4 mean GA), and 23 full-term born children, enrolled in the Preterm Asthma and Rhinitis (PRE-AR) ongoing longitudinal study at IBIM. An innovative approach based on nanochip liquid chromatography combined to high resolution mass spectrometry was used to obtain the proteomics profiles. Results: Proteomics analyses permitted the identification of 300 proteins in each urine sample, with a good repeatability (R2>0.99) and these are used for grouping the samples in relation to the different disease phenotypes. Conclusions: Obtained data indicate the proteomics approach as a promising platform for characterizing specific profiles related to PBP. These results represent a proof of principle for the proteomics application to all PRE-AR cases: along with clinical information, FeNO and lung function tests, this approach will attempt to discover candidate biomarkers and related metabolic pathways.

AB - Background: Preterms and twins are at higher risk of respiratory morbidity later in life. Advances in proteomic approaches may allow the characterization of biomarkers involved in respiratory diseases (Mauri et al. Imm. Lett. 2014;162:2-10). Gel-free approach quantitatively identify differentially expressed proteins in relation to physiopathological conditions (Mauri&Dehò, Meth Enzymology 2008;447:99-117). This can improve the clinical reliability of the next generation of biomarkers to discriminate multiple phenotypes of childhood respiratory diseases. Aim: To assess the ability of gel-free proteomics for identifying specific protein profiles related to PBP. Methods: Urine samples were collected from 3-5 years children: 43 prematurely born (33.7 mean gestational age - GA), including 20 twins (34.4 mean GA), and 23 full-term born children, enrolled in the Preterm Asthma and Rhinitis (PRE-AR) ongoing longitudinal study at IBIM. An innovative approach based on nanochip liquid chromatography combined to high resolution mass spectrometry was used to obtain the proteomics profiles. Results: Proteomics analyses permitted the identification of 300 proteins in each urine sample, with a good repeatability (R2>0.99) and these are used for grouping the samples in relation to the different disease phenotypes. Conclusions: Obtained data indicate the proteomics approach as a promising platform for characterizing specific profiles related to PBP. These results represent a proof of principle for the proteomics application to all PRE-AR cases: along with clinical information, FeNO and lung function tests, this approach will attempt to discover candidate biomarkers and related metabolic pathways.

KW - Preterm, children

UR - http://hdl.handle.net/10447/243305

M3 - Paper

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