INTRODUCTION AND OBJECTIVES: Epidermal Growth FactorReceptor (EGFR) overexpression has been recognized as a markerof biological aggressiveness in several malignant neoplasms. EGFRstudies on non-muscle invasive bladder cancer (NMIBC) are lacking.Urothelial EGFR status after TUR could provide useful informationabout the risk of recurrence and progression. The aim of our study wasto investigate the feasibility of EGFR evaluation in bladder washings ofpatients affected by NMIBC correlating its expression to EuropeanAssociation of Urology (EAU) risk classes.METHODS: A prospective double-blinded study included patientsundergoing adjuvant intravesical therapy for NMIBC and age-matchedhealthy controls. Acellular pelletwas obtained and stored at -80C. Isolationof cellular RNA was performed using a miRNeasy Mini Kit (Qiagen) andDNA derived from reverse transcription was used to perform gene expressionanalysis by a Real Time PCR according to ??Ct method.RESULTS: Fifty-eight patients and 21 controls entered thestudy. One hundred fifty-three samples were collected. A useful pelletwas obtained in 50 patients (86.2%) and 18 controls (85.7%). The meanEGFR expression in 50 NMIBC was 1.94 folds (range: 0.10-8.00).Compared with healthy controls (EGFR¼1), 40 patients (80%) werepositive for EGFR. Particularly, in 18 patients (36%) EGFR was overexpressed(EGFR>1.94) with a mean value of 3.61 folds (range: 2.00-8.00). According to EAU risk groups, EGFR expression was evaluatedin low, intermediate and high risk patients respectively. In 20 high riskNMIBC the mean EGFR expression resulted 2.45 folds and 10 of them(50%) presented significant up-regulation with a mean value of 4.10folds (range: 2.30-8.00). CONCLUSIONS: Our preliminary study demonstrates thefeasibility of EGFR determination in bladder washings of patientsaffected by NMIBC after TUR, during adjuvant treatment and subsequentfollow-up. A useful pellet was available in more than 85% ofpatients, with evidence of up-regulation in 36% of them. Within the highrisk category, a significant overexpression of EGFR, mean value of 4.16folds, was detected. EGFR overexpression might identify a subgroup ofhigh risk tumors inclined to progression despite of intravesical therapyand could represent an additional prognostic factor to select candidatesfor early radical cystectomy.
|Numero di pagine||2|
|Stato di pubblicazione||Published - 2016|