Extracellular Vesicles Shed by Melanoma Cells Contain a Modified Form of H1.0 Linker Histone and H1.0 mRNA-binding Proteins

Carlo Maria Di Liegro, Patrizia Cancemi, Italia Di Liegro, Anna Fricano, Gabriella Schiera, Gianluca Di Cara, Anna Fricano, Oriana Colletta, Veronica Puleo, Gabriella Schiera, Patrizia Cancemi, Carlo Maria Di Liegro

Risultato della ricerca: Conference articlepeer review

19 Citazioni (Scopus)


Extracellular vesicles (EVs) are shed in the extracellular environment by both prokaryotes and eukaryotes. Although produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which also contain tumor-specific proteins and RNAs.We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL1-2. Interestingly, G26/24 release, via EVs, extracellular matrix remodelling proteases3, and H1° histone protein4, and mRNA. To shed further light on the role of EVs in discarding proteins and mRNAs otherwise able to counteract proliferative signals, we studied a melanoma cell line (A375). We found that also these cancer cells produce H1° and release it into the extracellular space by EVs. Interestingly, H1° sorted to vesicles has a molecular mass higher than expected, and is probably sumoylated. By T1 RNase-protection assay with the H1° RNA, three main complexes were evidenced in EVs, the most abundant of which has a molecular mass of about 65 kDa. By using a biotinylated H1° RNA to fish interacting factors, we isolated from EVs a few proteins which have been then identified by mass spectrometry: the most abundant is a protein of about 60 kDa: myelin expression factor-2 (MYEF2). Western blot analyses confirmed the presence of MYEF2 in EVs released from A375 melanoma cells. 1. D’Agostino et al. 2006, Int J Oncol 29:1075-85.2. Lo Cicero A et al. 2011, Int J Oncol 39:1353-57. 3. Lo Cicero A et al. 2012, Matrix Biol 31: 229-33.4. Schiera G et al. 2013, Int J Oncol 43: 1771-76.
Lingua originaleEnglish
pagine (da-a)1807-1814
Numero di pagine8
RivistaEuropean Journal of Histochemistry
Stato di pubblicazionePublished - 2015

All Science Journal Classification (ASJC) codes

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