Extracellular vesicles shed by melanoma cells contain a modified form of H1.0 linker histone and H1.0 mRNA-binding proteins

Carlo Maria Di Liegro, Gabriella Schiera, Anna Fricano, Italia Di Liegro, Patrizia Cancemi, Gianluca Di Cara, Anna Fricano, Oriana Colletta, Veronica Puleo, Gabriella Schiera, Patrizia Cancemi, Carlo Maria Di Liegro

Risultato della ricerca: Articlepeer review

21 Citazioni (Scopus)


Extracellular vesicles (EVs) are now recognized as a fundamental way for cell-to-cell horizontal transfer of properties, in both physiological and pathological conditions. Most of EV-mediated cross-talk among cells depend on the exchange of proteins, and nucleic acids, among which mRNAs, and non-coding RNAs such as different species of miRNAs. Cancer cells, in particular, use EVs to discard molecules which could be dangerous to them (for example differentiation-inducing proteins such as histone H1.0, or antitumor drugs), to transfer molecules which, after entering the surrounding cells, are able to transform their phenotype, and even to secrete factors, which allow escaping from immune surveillance. Herein we report that melanoma cells not only secrete EVs which contain a modified form of H1.0 histone, but also transport the corresponding mRNA. Given the already known role in tumorigenesis of some RNA binding proteins (RBPs), we also searched for proteins of this class in EVs. This study revealed the presence in A375 melanoma cells of at least three RBPs, with apparent MW of about 65, 45 and 38 kDa, which are able to bind H1.0 mRNA. Moreover, we purified one of these proteins, which by MALDI-TOF mass spectrometry was identified as the already known transcription factor MYEF2.
Lingua originaleEnglish
pagine (da-a)1807-1814
Numero di pagine8
RivistaInternational Journal of Oncology
Stato di pubblicazionePublished - 2016

All Science Journal Classification (ASJC) codes

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