Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy

Antonio D'Amore, Julie A. Phillippi, Alkiviadis Tsamis, Antonio D'Amore, Jeffrey T. Krawiec, William R. Wagner, Ryan G. Koch, Patrick G. Chan, Simon C. Watkins, David A. Vorp, Thomas G. Gleason

    Risultato della ricerca: Article

    22 Citazioni (Scopus)

    Abstract

    Objective Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) commonly dilate asymmetrically compared with patients with tricuspid aortic valve (TAV). This discrepancy in aneurysm geometry led us to hypothesize that microarchitectural differences underlie the observed asymmetric dilatation pattern. The purpose of this study was to characterize the microarchitectural distinctions of the extracellular matrix of the 2 phenotypes with a focus on the proportion of radially oriented elastin and collagen fibers in different circumferential aortic regions. Methods Aortic tissue rings were obtained just distal to the sinotubular junction from patients with BAV or TAV undergoing elective aneurysm repair. They were sectioned into three circumferentially based regions according to adjacent aortic sinus segment (left coronary sinus [L], right coronary sinus [R], or noncoronary sinus [N]). Multiphoton microscopy was used to quantify and characterize the number of radially oriented elastin and collagen fibers. Results There were fewer radially oriented fibers in medial region N and medial-intimal region R of BAV-ATAAs when compared with TAV-ATAAs (medial region N, amplitude of angular undulation of elastin = 10.67° ± 1.35° vs 15.58° ± 1.91°; P =.041; medial-intimal region R, amplitude of angular undulation of elastin = 9.83° ± 0.83° vs 14.72° ± 1.64°; P =.015). Conversely, fibers became more radially oriented in the medial-intimal region L of BAV-ATAA when compared with TAV-ATAA (amplitude of angular undulation of collagen = 18.67° ± 0.95° vs 14.56° ± 1.37°; P =.041). Conclusions The differential pattern of fiber orientation noted between L and N-R regions help explain the unique pattern of greater curvature dilatation of BAV-ATAA. The distinctions noted in matrix microarchitecture may form the basis of differing aneurysm geometries and aortic wall integrities in ATAAs arising in these different valve morphologies.
    Lingua originaleEnglish
    pagine (da-a)1718-1728
    Numero di pagine11
    RivistaJournal of Thoracic and Cardiovascular Surgery
    Volume151
    Stato di pubblicazionePublished - 2016

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    Thoracic Aortic Aneurysm
    Tricuspid Valve
    Elastin
    Extracellular Matrix
    Tunica Intima
    Aortic Valve
    Collagen
    Coronary Sinus
    Aneurysm
    Dilatation
    Sinus of Valsalva
    Aortic Aneurysm
    Microscopy
    Bicuspid Aortic Valve
    Phenotype
    N-acetylVal-Nle(7,8)-allatotropin (5-13)

    All Science Journal Classification (ASJC) codes

    • Surgery
    • Pulmonary and Respiratory Medicine
    • Cardiology and Cardiovascular Medicine

    Cita questo

    D'Amore, A., Phillippi, J. A., Tsamis, A., D'Amore, A., Krawiec, J. T., Wagner, W. R., ... Gleason, T. G. (2016). Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy. Journal of Thoracic and Cardiovascular Surgery, 151, 1718-1728.

    Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy. / D'Amore, Antonio; Phillippi, Julie A.; Tsamis, Alkiviadis; D'Amore, Antonio; Krawiec, Jeffrey T.; Wagner, William R.; Koch, Ryan G.; Chan, Patrick G.; Watkins, Simon C.; Vorp, David A.; Gleason, Thomas G.

    In: Journal of Thoracic and Cardiovascular Surgery, Vol. 151, 2016, pag. 1718-1728.

    Risultato della ricerca: Article

    D'Amore, A, Phillippi, JA, Tsamis, A, D'Amore, A, Krawiec, JT, Wagner, WR, Koch, RG, Chan, PG, Watkins, SC, Vorp, DA & Gleason, TG 2016, 'Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy', Journal of Thoracic and Cardiovascular Surgery, vol. 151, pagg. 1718-1728.
    D'Amore, Antonio ; Phillippi, Julie A. ; Tsamis, Alkiviadis ; D'Amore, Antonio ; Krawiec, Jeffrey T. ; Wagner, William R. ; Koch, Ryan G. ; Chan, Patrick G. ; Watkins, Simon C. ; Vorp, David A. ; Gleason, Thomas G. / Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy. In: Journal of Thoracic and Cardiovascular Surgery. 2016 ; Vol. 151. pagg. 1718-1728.
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    title = "Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy",
    abstract = "Objective Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) commonly dilate asymmetrically compared with patients with tricuspid aortic valve (TAV). This discrepancy in aneurysm geometry led us to hypothesize that microarchitectural differences underlie the observed asymmetric dilatation pattern. The purpose of this study was to characterize the microarchitectural distinctions of the extracellular matrix of the 2 phenotypes with a focus on the proportion of radially oriented elastin and collagen fibers in different circumferential aortic regions. Methods Aortic tissue rings were obtained just distal to the sinotubular junction from patients with BAV or TAV undergoing elective aneurysm repair. They were sectioned into three circumferentially based regions according to adjacent aortic sinus segment (left coronary sinus [L], right coronary sinus [R], or noncoronary sinus [N]). Multiphoton microscopy was used to quantify and characterize the number of radially oriented elastin and collagen fibers. Results There were fewer radially oriented fibers in medial region N and medial-intimal region R of BAV-ATAAs when compared with TAV-ATAAs (medial region N, amplitude of angular undulation of elastin = 10.67° ± 1.35° vs 15.58° ± 1.91°; P =.041; medial-intimal region R, amplitude of angular undulation of elastin = 9.83° ± 0.83° vs 14.72° ± 1.64°; P =.015). Conversely, fibers became more radially oriented in the medial-intimal region L of BAV-ATAA when compared with TAV-ATAA (amplitude of angular undulation of collagen = 18.67° ± 0.95° vs 14.56° ± 1.37°; P =.041). Conclusions The differential pattern of fiber orientation noted between L and N-R regions help explain the unique pattern of greater curvature dilatation of BAV-ATAA. The distinctions noted in matrix microarchitecture may form the basis of differing aneurysm geometries and aortic wall integrities in ATAAs arising in these different valve morphologies.",
    keywords = "80 and over; Aorta, Thoracic; Aortic Aneurysm, Thoracic; Aortic Valve; Elasticity; Extracellular Matrix; Female; Heart Valve Diseases; Humans; Male; Middle Aged; Cardiology and Cardiovascular Medicine; Surgery; Pulmonary and Respiratory Medicine, aortic wall microarchitecture; aortopathy; ascending aortic aneurysm; bicuspid aortic valve; collagen; elastin; Adult; Aged; Aged",
    author = "Antonio D'Amore and Phillippi, {Julie A.} and Alkiviadis Tsamis and Antonio D'Amore and Krawiec, {Jeffrey T.} and Wagner, {William R.} and Koch, {Ryan G.} and Chan, {Patrick G.} and Watkins, {Simon C.} and Vorp, {David A.} and Gleason, {Thomas G.}",
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    language = "English",
    volume = "151",
    pages = "1718--1728",
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    TY - JOUR

    T1 - Extracellular matrix fiber microarchitecture is region-specific in bicuspid aortic valve-associated ascending aortopathy

    AU - D'Amore, Antonio

    AU - Phillippi, Julie A.

    AU - Tsamis, Alkiviadis

    AU - D'Amore, Antonio

    AU - Krawiec, Jeffrey T.

    AU - Wagner, William R.

    AU - Koch, Ryan G.

    AU - Chan, Patrick G.

    AU - Watkins, Simon C.

    AU - Vorp, David A.

    AU - Gleason, Thomas G.

    PY - 2016

    Y1 - 2016

    N2 - Objective Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) commonly dilate asymmetrically compared with patients with tricuspid aortic valve (TAV). This discrepancy in aneurysm geometry led us to hypothesize that microarchitectural differences underlie the observed asymmetric dilatation pattern. The purpose of this study was to characterize the microarchitectural distinctions of the extracellular matrix of the 2 phenotypes with a focus on the proportion of radially oriented elastin and collagen fibers in different circumferential aortic regions. Methods Aortic tissue rings were obtained just distal to the sinotubular junction from patients with BAV or TAV undergoing elective aneurysm repair. They were sectioned into three circumferentially based regions according to adjacent aortic sinus segment (left coronary sinus [L], right coronary sinus [R], or noncoronary sinus [N]). Multiphoton microscopy was used to quantify and characterize the number of radially oriented elastin and collagen fibers. Results There were fewer radially oriented fibers in medial region N and medial-intimal region R of BAV-ATAAs when compared with TAV-ATAAs (medial region N, amplitude of angular undulation of elastin = 10.67° ± 1.35° vs 15.58° ± 1.91°; P =.041; medial-intimal region R, amplitude of angular undulation of elastin = 9.83° ± 0.83° vs 14.72° ± 1.64°; P =.015). Conversely, fibers became more radially oriented in the medial-intimal region L of BAV-ATAA when compared with TAV-ATAA (amplitude of angular undulation of collagen = 18.67° ± 0.95° vs 14.56° ± 1.37°; P =.041). Conclusions The differential pattern of fiber orientation noted between L and N-R regions help explain the unique pattern of greater curvature dilatation of BAV-ATAA. The distinctions noted in matrix microarchitecture may form the basis of differing aneurysm geometries and aortic wall integrities in ATAAs arising in these different valve morphologies.

    AB - Objective Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) commonly dilate asymmetrically compared with patients with tricuspid aortic valve (TAV). This discrepancy in aneurysm geometry led us to hypothesize that microarchitectural differences underlie the observed asymmetric dilatation pattern. The purpose of this study was to characterize the microarchitectural distinctions of the extracellular matrix of the 2 phenotypes with a focus on the proportion of radially oriented elastin and collagen fibers in different circumferential aortic regions. Methods Aortic tissue rings were obtained just distal to the sinotubular junction from patients with BAV or TAV undergoing elective aneurysm repair. They were sectioned into three circumferentially based regions according to adjacent aortic sinus segment (left coronary sinus [L], right coronary sinus [R], or noncoronary sinus [N]). Multiphoton microscopy was used to quantify and characterize the number of radially oriented elastin and collagen fibers. Results There were fewer radially oriented fibers in medial region N and medial-intimal region R of BAV-ATAAs when compared with TAV-ATAAs (medial region N, amplitude of angular undulation of elastin = 10.67° ± 1.35° vs 15.58° ± 1.91°; P =.041; medial-intimal region R, amplitude of angular undulation of elastin = 9.83° ± 0.83° vs 14.72° ± 1.64°; P =.015). Conversely, fibers became more radially oriented in the medial-intimal region L of BAV-ATAA when compared with TAV-ATAA (amplitude of angular undulation of collagen = 18.67° ± 0.95° vs 14.56° ± 1.37°; P =.041). Conclusions The differential pattern of fiber orientation noted between L and N-R regions help explain the unique pattern of greater curvature dilatation of BAV-ATAA. The distinctions noted in matrix microarchitecture may form the basis of differing aneurysm geometries and aortic wall integrities in ATAAs arising in these different valve morphologies.

    KW - 80 and over; Aorta

    KW - Thoracic; Aortic Aneurysm

    KW - Thoracic; Aortic Valve; Elasticity; Extracellular Matrix; Female; Heart Valve Diseases; Humans; Male; Middle Aged; Cardiology and Cardiovascular Medicine; Surgery; Pulmonary and Respiratory Medicine

    KW - aortic wall microarchitecture; aortopathy; ascending aortic aneurysm; bicuspid aortic valve; collagen; elastin; Adult; Aged; Aged

    UR - http://hdl.handle.net/10447/222137

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    M3 - Article

    VL - 151

    SP - 1718

    EP - 1728

    JO - Journal of Thoracic and Cardiovascular Surgery

    JF - Journal of Thoracic and Cardiovascular Surgery

    SN - 0022-5223

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