Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway

Gabriella Sconzo, Fabiana Geraci, Walter Spinello, Maria Magdalena Barreca, Vincenzo Cavalieri, Rosaria Tinnirello, Giuseppina Turturici, Rosaria Tinnirello, Punit Kaur, Fabiana Geraci, Alexzander A. A. Asea

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).
Lingua originaleEnglish
Numero di pagine0
RivistaJournal of Cellular Physiology
Stato di pubblicazionePublished - 2017

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Autocrine Communication
Matrix Metalloproteinase 2
Stem cells
Stem Cells
Tissue
Signal transduction
Toll-Like Receptor 4
Extracellular Matrix
Phosphatidylinositol 3-Kinases
Chemokines
Blood
Mesoderm
Cytokines
Membranes
Cell Movement
Regeneration
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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title = "Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway",
abstract = "Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).",
author = "Gabriella Sconzo and Fabiana Geraci and Walter Spinello and Barreca, {Maria Magdalena} and Vincenzo Cavalieri and Rosaria Tinnirello and Giuseppina Turturici and Rosaria Tinnirello and Punit Kaur and Fabiana Geraci and Asea, {Alexzander A. A.}",
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TY - JOUR

T1 - Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway

AU - Sconzo, Gabriella

AU - Geraci, Fabiana

AU - Spinello, Walter

AU - Barreca, Maria Magdalena

AU - Cavalieri, Vincenzo

AU - Tinnirello, Rosaria

AU - Turturici, Giuseppina

AU - Tinnirello, Rosaria

AU - Kaur, Punit

AU - Geraci, Fabiana

AU - Asea, Alexzander A. A.

PY - 2017

Y1 - 2017

N2 - Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).

AB - Mouse mesoangioblasts are vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. Therefore, they represent a promising tool in the regeneration of injured tissues. Several studies have demonstrated that homing of mesoangioblasts into blood and injured tissues are mainly controlled by cytokines/chemokines and other inflammatory factors. However, little is known about the molecular mechanisms regulating their ability to traverse the extracellular matrix (ECM). Here, we demonstrate that membrane vesicles released by mesoangioblasts contain Hsp70, and that the released Hsp70 is able to interact by an autocrine mechanism with Toll-like receptor 4 (TLR4) and CD91 to stimulate migration. We further demonstrate that Hsp70 has a positive role in regulating matrix metalloproteinase 2 (MMP2) and MMP9 expression and that MMP2 has a more pronounced effect on cell migration, as compared to MMP9. In addition, the analysis of the intracellular pathways implicated in Hsp70 regulated signal transduction showed the involvement of both PI3K/AKT and NF-κB. Taken together, our findings present a paradigm shift in our understanding of the molecular mechanisms that regulate mesoangioblast stem cells ability to traverse the extracellular matrix (ECM).

UR - http://hdl.handle.net/10447/215873

M3 - Article

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

ER -