Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples

Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M

Risultato della ricerca: Paper

Abstract

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 through immunohistochemistry and gene expression through RT-PCR analysis. Results. In control sample we identified a weak expression of both metalloproteinases (MMP-2, MMP-9) in the upper epithelial layers. The reactivity of the two enzymes is emphasized with higher intensity in pathological samples where a strong inflammatory activity is present (e.g. hyperplasia, dysplasia) or where there is a distruption of the architecture (as in tumors) while in localized lesions a functional silence is detected (mucocele). Particularly, in lesions like hyperplasia and dysplasia where (though it is present a inflammatory condition) the bio-architecture is preserved, the immunoreactivity is expressed only in the upper epithelial cell layers; while in more serious lesions (like tumors), where the architecture is fully disorganizated, the immunoreactivity is expressed also in deeper epithelial layers, in few connnective cells and in vessels wall. Qualitative RT-PCR analysis confirmed the expression of MMP-2 and MMP-9 in both normal and pathological oral mucosa samples. Conclusions. Our data suggest that there is a relationship between the increase of MMP-2 and MMP-9 expression and the entity of morphological disorganization according with the well known role of these enzymes.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2008

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Mouth Mucosa
Matrix Metalloproteinases
Enzymes
Hyperplasia
Mucocele
Matrix Metalloproteinase 2
Polymerase Chain Reaction
Immunohistochemistry
Neoplasms
Fibroma
Mouth
Epithelial Cells
Carcinoma
Gene Expression
Wounds and Injuries

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Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M (2008). Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples.

Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples. / Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M.

2008.

Risultato della ricerca: Paper

Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M 2008, 'Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples'.
Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M. Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples. 2008.
Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M. / Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples.
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abstract = "Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 through immunohistochemistry and gene expression through RT-PCR analysis. Results. In control sample we identified a weak expression of both metalloproteinases (MMP-2, MMP-9) in the upper epithelial layers. The reactivity of the two enzymes is emphasized with higher intensity in pathological samples where a strong inflammatory activity is present (e.g. hyperplasia, dysplasia) or where there is a distruption of the architecture (as in tumors) while in localized lesions a functional silence is detected (mucocele). Particularly, in lesions like hyperplasia and dysplasia where (though it is present a inflammatory condition) the bio-architecture is preserved, the immunoreactivity is expressed only in the upper epithelial cell layers; while in more serious lesions (like tumors), where the architecture is fully disorganizated, the immunoreactivity is expressed also in deeper epithelial layers, in few connnective cells and in vessels wall. Qualitative RT-PCR analysis confirmed the expression of MMP-2 and MMP-9 in both normal and pathological oral mucosa samples. Conclusions. Our data suggest that there is a relationship between the increase of MMP-2 and MMP-9 expression and the entity of morphological disorganization according with the well known role of these enzymes.",
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author = "{Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M} and Silvia Tortorici and Angelo Leone and Spatola, {Giovanni Francesco} and Annamaria Mauro",
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T1 - Expression of remodelling enzymes (MMP-2, MMP-9) in normal and pathological oral mucosa samples

AU - Lipari L, Mauro A, Leone A, Spatola Gf, Gerbino A, Burruano F, Tortorici S, Buscemi M

AU - Tortorici, Silvia

AU - Leone, Angelo

AU - Spatola, Giovanni Francesco

AU - Mauro, Annamaria

PY - 2008

Y1 - 2008

N2 - Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 through immunohistochemistry and gene expression through RT-PCR analysis. Results. In control sample we identified a weak expression of both metalloproteinases (MMP-2, MMP-9) in the upper epithelial layers. The reactivity of the two enzymes is emphasized with higher intensity in pathological samples where a strong inflammatory activity is present (e.g. hyperplasia, dysplasia) or where there is a distruption of the architecture (as in tumors) while in localized lesions a functional silence is detected (mucocele). Particularly, in lesions like hyperplasia and dysplasia where (though it is present a inflammatory condition) the bio-architecture is preserved, the immunoreactivity is expressed only in the upper epithelial cell layers; while in more serious lesions (like tumors), where the architecture is fully disorganizated, the immunoreactivity is expressed also in deeper epithelial layers, in few connnective cells and in vessels wall. Qualitative RT-PCR analysis confirmed the expression of MMP-2 and MMP-9 in both normal and pathological oral mucosa samples. Conclusions. Our data suggest that there is a relationship between the increase of MMP-2 and MMP-9 expression and the entity of morphological disorganization according with the well known role of these enzymes.

AB - Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 through immunohistochemistry and gene expression through RT-PCR analysis. Results. In control sample we identified a weak expression of both metalloproteinases (MMP-2, MMP-9) in the upper epithelial layers. The reactivity of the two enzymes is emphasized with higher intensity in pathological samples where a strong inflammatory activity is present (e.g. hyperplasia, dysplasia) or where there is a distruption of the architecture (as in tumors) while in localized lesions a functional silence is detected (mucocele). Particularly, in lesions like hyperplasia and dysplasia where (though it is present a inflammatory condition) the bio-architecture is preserved, the immunoreactivity is expressed only in the upper epithelial cell layers; while in more serious lesions (like tumors), where the architecture is fully disorganizated, the immunoreactivity is expressed also in deeper epithelial layers, in few connnective cells and in vessels wall. Qualitative RT-PCR analysis confirmed the expression of MMP-2 and MMP-9 in both normal and pathological oral mucosa samples. Conclusions. Our data suggest that there is a relationship between the increase of MMP-2 and MMP-9 expression and the entity of morphological disorganization according with the well known role of these enzymes.

KW - MMP-2, MMp-9, oral mucosa

UR - http://hdl.handle.net/10447/35040

M3 - Paper

ER -