TY - JOUR
T1 - Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in normal and pathological human oral mucosa
AU - Buscemi, Maria
AU - Gerbino, Aldo
AU - Burruano, Francesco
AU - Tortorici, Silvia
AU - Leone, Angelo
AU - Lipari, Luana
AU - Mauro, Annamaria
AU - Provenzano, Salvatore
AU - Lipari, Luana
AU - Burruano, Francesco
AU - Gerbino, Aldo
AU - Buscemi, Maria
AU - Tortorici, Silvia
AU - Leone, Angelo
AU - Mauro, Annamaria
AU - Provenzano, Salvatore
PY - 2010
Y1 - 2010
N2 - Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation.
The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with the various stages of oral cancer progression.
Since COX enzyme has been involved, with different mechanisms, in the development and progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2 in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistry and RT-PCR.
COX-1 mRNA and protein have been detected already in normal oral mucosa and their expression progressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma.
On the contrary, COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasia and then starts to be downregulated in carcinoma.
AB - Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins (PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cell homeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is strongly expressed in inflammation.
The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosal reactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positive outcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with the various stages of oral cancer progression.
Since COX enzyme has been involved, with different mechanisms, in the development and progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2 in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistry and RT-PCR.
COX-1 mRNA and protein have been detected already in normal oral mucosa and their expression progressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma.
On the contrary, COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasia and then starts to be downregulated in carcinoma.
KW - Cyclooxigenases, human oral mucosa, hyperplasia, dysplasia, carcinoma.
UR - http://hdl.handle.net/10447/53052
M3 - Article
VL - 48
SP - 555
EP - 563
JO - Folia Histochemica et Cytobiologica
JF - Folia Histochemica et Cytobiologica
SN - 0239-8508
ER -