EXPRESSION of Cyclooxygenase-1 and cyclooxigenase-2 in normal and pathological human oral mucosa

Risultato della ricerca: Article

14 Citazioni (Scopus)

Abstract

Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins(PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cellhomeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is stronglyexpressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosalreactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positiveoutcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with thevarious stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the developmentand progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistryand RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expressionprogressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary,COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasiaand then starts to be downregulated in carcinoma.
Lingua originaleEnglish
pagine (da-a)555-563
Numero di pagine9
RivistaFolia Histochemica et Cytobiologica
Volume48
Stato di pubblicazionePublished - 2010

Fingerprint

Cyclooxygenase 1
Mouth Mucosa
Hyperplasia
Mouth Neoplasms
Carcinoma
Enzymes
Arachidonic Acid
Prostaglandins
Mouth
Early Diagnosis
Neoplasms
Protein Isoforms
Down-Regulation
Pathology
Inflammation
Polymerase Chain Reaction
Messenger RNA
Wounds and Injuries
Proteins

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cita questo

@article{834aaee4d8cf4731b6a219e9373cce2c,
title = "EXPRESSION of Cyclooxygenase-1 and cyclooxigenase-2 in normal and pathological human oral mucosa",
abstract = "Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins(PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cellhomeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is stronglyexpressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosalreactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positiveoutcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with thevarious stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the developmentand progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistryand RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expressionprogressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary,COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasiaand then starts to be downregulated in carcinoma.",
keywords = "carcinoma, cyclooxigenases, dysplasia, human oral mucosa, hyperplasia",
author = "Angelo Leone and Aldo Gerbino and Maria Buscemi and Francesco Burruano and Silvia Tortorici and Annamaria Mauro and Luana Lipari and Salvatore Provenzano",
year = "2010",
language = "English",
volume = "48",
pages = "555--563",
journal = "Folia Histochemica et Cytobiologica",
issn = "0239-8508",
publisher = "Polish Histochemical and Cytochemical Society",

}

TY - JOUR

T1 - EXPRESSION of Cyclooxygenase-1 and cyclooxigenase-2 in normal and pathological human oral mucosa

AU - Leone, Angelo

AU - Gerbino, Aldo

AU - Buscemi, Maria

AU - Burruano, Francesco

AU - Tortorici, Silvia

AU - Mauro, Annamaria

AU - Lipari, Luana

AU - Provenzano, Salvatore

PY - 2010

Y1 - 2010

N2 - Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins(PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cellhomeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is stronglyexpressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosalreactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positiveoutcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with thevarious stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the developmentand progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistryand RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expressionprogressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary,COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasiaand then starts to be downregulated in carcinoma.

AB - Abstract: Cyclooxigenase (COX) is the rate-limiting enzyme for the conversion of arachidonic acid (AA) to prostaglandins(PGs). Two isoforms of COX have been identified: COX-1 is constitutively expressed in many cells and is involved in cellhomeostasis, angiogenesis and cell-cell signalling; COX-2 is not expressed in normal condition however it is stronglyexpressed in inflammation. The oral cavity is costantly exposed to physical and chemical trauma that could lead to mucosalreactions such as hyperplasia, dysplasia and cancer. Early diagnosis is the most important issue to address for a positiveoutcome of oral cancer; therefore it would be useful to identify molecular markers whose expression is associated with thevarious stages of oral cancer progression. Since COX enzyme has been involved, with different mechanisms, in the developmentand progression of malignancies we decided to investigate the expression and localization of COX-1 and COX-2in normal human oral mucosa and three different pathologies (hyperplasia, dysplasia and carcinoma) by immunohistochemistryand RT-PCR. COX-1 mRNA and protein have been detected already in normal oral mucosa and their expressionprogressively increases from normal samples towards hyperplasia, dysplasia and finally carcinoma. On the contrary,COX-2 is not expressed in the normal tissue, starts to be expressed in hyperplasia, reaches the maximum activation in dysplasiaand then starts to be downregulated in carcinoma.

KW - carcinoma

KW - cyclooxigenases

KW - dysplasia

KW - human oral mucosa

KW - hyperplasia

UR - http://hdl.handle.net/10447/58272

M3 - Article

VL - 48

SP - 555

EP - 563

JO - Folia Histochemica et Cytobiologica

JF - Folia Histochemica et Cytobiologica

SN - 0239-8508

ER -