INTRODUCTION: Post-transcriptional regulation of gene expression relies on RNA-binding proteins(RBPs), which regulate intracellular transport, stability, and translation of mRNAs . We previously identifieda set of proteins which interact with mRNAs encoding H1° and H3.3 histones [2-5]. All these proteinsare probably part of a ribonucleoprotein particle . Here we report more details on the expression andintracellular localization of some of these RBPs, during rat brain development and in isolated rat astrocytes.METHODS: Affinity chromatography was performed as already described . Preparation of total lysatesand cellular sub-fractions was done as reported in . Possible co-localization of Hsc70 with CSD-C2 in culturedastrocytes was analysed by immunofluorescence microscopy.RESULTS: The presence of Hsc70 chaperone in the already identified ribonucleoprotein complex  wasconfirmed by affinity chromatography. We also found that the complex itself is present not only in the nuclearextracts, but also in the cytoplasmic fraction. Moreover, A1 and K hnRNPs, previously found in the complexes,were found to be differentially expressed and localized during rat brain maturation; an increase in A1expression was also demonstrated in cultured astrocytes grown on a fibronectin-containing substrate.We finally report that sumoylated PIPPin, already found in neurons, is also present in the nuclei of culturedastrocytes.CONCLUSIONS: We confirmed the existence of a group of proteins able to interact with H1°/H3.3 mRNAs.These proteins are, however, differentially expressed during brain maturation and also show different subcellularlocalization. Di Liegro et al. 2014, Int J Mol Med 33:747-62. ;  Scaturro et al. 1998, J Biol Chem 273:22788-91.; Nastasi et al. 1999, J Biol Chem 274:24087-93.  Sala et al. 2007, Int J Mol Med 19:501-9.  Saladino et al.2012, Int J Mol Med 29:141-5  Di Liegro et al. 2013, Neuroscience 229:71-6.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2015|