Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg

Tabone, M; Imparato, V

Risultato della ricerca: Paper

Abstract

Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75% similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2009

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Gordonia (bacteria)
alkanes
metabolism
genes
waxes
Nocardia farcinica
CoA-transferases
diacylglycerol acyltransferase
hexadecane
chromosomes
wax esters
Acinetobacter
synthesis
Sicily
Gram-positive bacteria
oligonucleotides
palmitic acid
beaches
hydrocarbons
reverse transcriptase polymerase chain reaction

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Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg. / Tabone, M; Imparato, V.

2009.

Risultato della ricerca: Paper

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title = "Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg",
abstract = "Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75{\%} similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.",
keywords = "long chain n-alkanes; Gordonia; biodegradation; alkane-monoxigenase; alk genes;",
author = "{Tabone, M; Imparato, V} and Puglia, {Anna Maria} and Paola Quatrini and {De Pasquale}, Claudio and Roberta Fodale and {Lo Piccolo}, Luca",
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T1 - Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg

AU - Tabone, M; Imparato, V

AU - Puglia, Anna Maria

AU - Quatrini, Paola

AU - De Pasquale, Claudio

AU - Fodale, Roberta

AU - Lo Piccolo, Luca

PY - 2009

Y1 - 2009

N2 - Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75% similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.

AB - Many microorganisms are able to degrade aliphatic hydrocarbons and a relationship between n-alkane utilization and storage compound synthesis has been described in bacteria. The Gram positive GC-rich n-alkane degrader Gordonia sp. strain SoCg, isolated from a long-term accidentally contaminated beach in Sicily, is able to grow on long n-alkanes up to. It carries a single copy of the alkane hydroxylase gene alkB on its chromosome and its alk cluster revealed a genomic organization similar to other alk clusters of alkane-degrading Gram positive bacteria. The alk gene expression, analysed by Real-time RT-PCR, is induced by n-hexadecane and n-triacontane and coupled to alkane consumption. Interestingly, SPME GC-MS analysis revealed extracellular production of hexadecyl-hexadecanoic acid (wax ester), during growth on n-triacontane. Degenerated oligonucleotides were used to PCR amplify the Wax Esther Synthase/Acyl-CoA:Diacylglycerol Acyltransferase encoding gene (atfa), responsible for intracellular wax esther synthesis by estherification between palmitoyl-CoA and 1-haxadecanol in the n-alkane degrader Acinetobacter sp. ADP1. The amplicon showed 75% similarity sequence to a putative acyl-CoA transferase gene of Nocardia farcinica. Only one copy of atfa-like gene was detected in Gordonia SoCg chromosome. qRT-PCR analysis showed an up-regulation of atfa gene in the presence of long chain n-alkanes, correlating, for the first time, long chain n-alkane metabolism and wax esthers extracellular production.

KW - long chain n-alkanes; Gordonia; biodegradation; alkane-monoxigenase; alk genes;

UR - http://hdl.handle.net/10447/57173

M3 - Paper

ER -