Exploration of the Sea Urchin Coelomic Fluid via Combinatorial Peptide Ligand Libraries.

Rainero Barbieri, Elisa Fasoli, Pier Giorgio Righetti, Alfonsina D'Amato, Daniele Bellavia

    Risultato della ricerca: Articlepeer review

    8 Citazioni (Scopus)

    Abstract

    The urchin Paracentrotus lividus has been characterized via previous capture and enhancement of low-abundance proteins with combinatorial peptide ligand libraries (CPLL, ProteoMiner). Whereas in the control only 26 unique gene products could be identified, 82 species could be detected after CPLL treatment. Due to the overwhelming presence of two major proteins-the toposome (a highly glycosylated, modified calcium-binding, iron-less transferrin) and the major yolk proteins, belonging to the class of cell adhesion proteins-which constituted about 70% of the proteome of this biological fluid and strongly interfered with the capture of the minority proteome, no additional proteins could be detected. Yet, at present, this constitutes the most thorough investigation of the proteome of this biological fluid.
    Lingua originaleEnglish
    pagine (da-a)93-104
    Numero di pagine12
    RivistaTHE BIOLOGICAL BULLETIN
    Volume222
    Stato di pubblicazionePublished - 2012

    All Science Journal Classification (ASJC) codes

    • ???subjectarea.asjc.1100.1100???

    Fingerprint Entra nei temi di ricerca di 'Exploration of the Sea Urchin Coelomic Fluid via Combinatorial Peptide Ligand Libraries.'. Insieme formano una fingerprint unica.

    Cita questo