Expansion of intracellular IFN-g positive lymphocytes duringMycoplasma agalactiae infection in sheep

Francesco Dieli, Annalisa Agnone, Guido Sireci, Marco Pio La Manna, Maria Vitale, Sara Villari, Robin A.J. Nicholas, Roberto Puleio, Guido R. Loria

Risultato della ricerca: Articlepeer review

6 Citazioni (Scopus)


A method to assess the expansion of antigen-specific intracellular IFN-c positive T cell subsets during theinfection will be helpful for a better understanding of mycoplasmal infections physiopathology in thesheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-c duringthe infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells ofinfected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-c positivelymphocytes in infected sheep was initially sustained by CD4+ T cells at day 15 after infection, whenantigen specific IgG start to be detectable, followed by CD8/IFN-c double positive cells. cd T-cells werenot expanded at any time point analysed. IFNc+ T cells disappear 60 days after infection, suggesting thatantigen specific IFNc+ T cells, mainly detected in the early phase of the disease, could be useful to understandthe role of cell-mediated immunity during M. agalactiae infection.
Lingua originaleEnglish
pagine (da-a)64-67
Numero di pagine4
RivistaResearch in Veterinary Science
Stato di pubblicazionePublished - 2011

All Science Journal Classification (ASJC) codes

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