A method to assess the expansion of antigen-specific intracellular IFN-c positive T cell subsets during theinfection will be helpful for a better understanding of mycoplasmal infections physiopathology in thesheep. We analysed the percentage of antigen-specific lymphocytes positive for intracellular IFN-c duringthe infection of sheep with Mycoplasma agalactiae by culturing peripheral blood mononuclear cells ofinfected or uninfected animals with irradiated M. agalactiae. The expansion of antigen-specific IFN-c positivelymphocytes in infected sheep was initially sustained by CD4+ T cells at day 15 after infection, whenantigen specific IgG start to be detectable, followed by CD8/IFN-c double positive cells. cd T-cells werenot expanded at any time point analysed. IFNc+ T cells disappear 60 days after infection, suggesting thatantigen specific IFNc+ T cells, mainly detected in the early phase of the disease, could be useful to understandthe role of cell-mediated immunity during M. agalactiae infection.
|Numero di pagine||4|
|Rivista||Research in Veterinary Science|
|Stato di pubblicazione||Published - 2011|
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