Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses

Giovanni Giammanco, Simona De Grazia, Vito Martella, Marc Van Ranst, Kristián Bányai, Jelle Matthijnssens, Mark Zeller, Elisabeth Heylen

Risultato della ricerca: Article

29 Citazioni (Scopus)

Abstract

Group A rotaviruses (RVAs) are a leading cause of viral gastroenteritis in children, with G2P[4] RVA being one of the most common human strains worldwide. The complete genome sequences of nine G2P[4] RVA strains, selected from a 26-year archival collection (1985-2011) established in Palermo, Italy, were determined. A strain associated with a peak of G2P[4] RVA activity in 1996 resembled a reassortant strain identified in Kenya in 1982 and differed completely in genomic make up from more recent strains that circulated during 2004-2011. Conversely, the 2004-2011 G2P[4] RVAs were genetically more similar to contemporary RVA strains circulating globally. Recent G2P[4] strains possessed either single or multiple genome segments (VP1, VP3 and/or NSP4) likely derived from ruminant viruses through intra-genotype reassortment. Amino acid substitutions were selected and maintained over time in the VP7 and VP8* antigenic proteins, allowing the circulation of two contemporary G2P[4] variants to be distinguished. Altogether, these findings suggest that major changes in the genomic composition of recent G2P[4] RVAs occurred in the early 2000s, leading to the appearance of a novel variant of the DS-1-like genotype constellation. Whether the modifications observed in the neutralizing antigens and in the genome composition of modern G2P[4] RVAs may affect the long-term effectiveness of the vaccination programmes remains to be explored.
Lingua originaleEnglish
pagine (da-a)91-109
Numero di pagine19
RivistaJournal of General Virology
Volume95
Stato di pubblicazionePublished - 2014

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Rotavirus
Genome
Genotype
Kenya
Program Evaluation
Gastroenteritis
Ruminants
Amino Acid Substitution
Italy
Vaccination
Viruses
Antigens
Proteins

All Science Journal Classification (ASJC) codes

  • Virology

Cita questo

Giammanco, G., De Grazia, S., Martella, V., Van Ranst, M., Bányai, K., Matthijnssens, J., ... Heylen, E. (2014). Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses. Journal of General Virology, 95, 91-109.

Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses. / Giammanco, Giovanni; De Grazia, Simona; Martella, Vito; Van Ranst, Marc; Bányai, Kristián; Matthijnssens, Jelle; Zeller, Mark; Heylen, Elisabeth.

In: Journal of General Virology, Vol. 95, 2014, pag. 91-109.

Risultato della ricerca: Article

Giammanco, G, De Grazia, S, Martella, V, Van Ranst, M, Bányai, K, Matthijnssens, J, Zeller, M & Heylen, E 2014, 'Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses', Journal of General Virology, vol. 95, pagg. 91-109.
Giammanco, Giovanni ; De Grazia, Simona ; Martella, Vito ; Van Ranst, Marc ; Bányai, Kristián ; Matthijnssens, Jelle ; Zeller, Mark ; Heylen, Elisabeth. / Evolution of DS-1-like human G2P[4] rotaviruses assessed by complete genome analyses. In: Journal of General Virology. 2014 ; Vol. 95. pagg. 91-109.
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abstract = "Group A rotaviruses (RVAs) are a leading cause of viral gastroenteritis in children, with G2P[4] RVA being one of the most common human strains worldwide. The complete genome sequences of nine G2P[4] RVA strains, selected from a 26-year archival collection (1985-2011) established in Palermo, Italy, were determined. A strain associated with a peak of G2P[4] RVA activity in 1996 resembled a reassortant strain identified in Kenya in 1982 and differed completely in genomic make up from more recent strains that circulated during 2004-2011. Conversely, the 2004-2011 G2P[4] RVAs were genetically more similar to contemporary RVA strains circulating globally. Recent G2P[4] strains possessed either single or multiple genome segments (VP1, VP3 and/or NSP4) likely derived from ruminant viruses through intra-genotype reassortment. Amino acid substitutions were selected and maintained over time in the VP7 and VP8* antigenic proteins, allowing the circulation of two contemporary G2P[4] variants to be distinguished. Altogether, these findings suggest that major changes in the genomic composition of recent G2P[4] RVAs occurred in the early 2000s, leading to the appearance of a novel variant of the DS-1-like genotype constellation. Whether the modifications observed in the neutralizing antigens and in the genome composition of modern G2P[4] RVAs may affect the long-term effectiveness of the vaccination programmes remains to be explored.",
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AU - Van Ranst, Marc

AU - Bányai, Kristián

AU - Matthijnssens, Jelle

AU - Zeller, Mark

AU - Heylen, Elisabeth

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AB - Group A rotaviruses (RVAs) are a leading cause of viral gastroenteritis in children, with G2P[4] RVA being one of the most common human strains worldwide. The complete genome sequences of nine G2P[4] RVA strains, selected from a 26-year archival collection (1985-2011) established in Palermo, Italy, were determined. A strain associated with a peak of G2P[4] RVA activity in 1996 resembled a reassortant strain identified in Kenya in 1982 and differed completely in genomic make up from more recent strains that circulated during 2004-2011. Conversely, the 2004-2011 G2P[4] RVAs were genetically more similar to contemporary RVA strains circulating globally. Recent G2P[4] strains possessed either single or multiple genome segments (VP1, VP3 and/or NSP4) likely derived from ruminant viruses through intra-genotype reassortment. Amino acid substitutions were selected and maintained over time in the VP7 and VP8* antigenic proteins, allowing the circulation of two contemporary G2P[4] variants to be distinguished. Altogether, these findings suggest that major changes in the genomic composition of recent G2P[4] RVAs occurred in the early 2000s, leading to the appearance of a novel variant of the DS-1-like genotype constellation. Whether the modifications observed in the neutralizing antigens and in the genome composition of modern G2P[4] RVAs may affect the long-term effectiveness of the vaccination programmes remains to be explored.

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