Evidence of cytotoxic activity against mammalian red blood cell of Na+ channel neurotoxin (AE1) from sea anemone (actinia equina)

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Abstract

The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.
Lingua originaleEnglish
pagine (da-a)309-314
Numero di pagine6
RivistaInvertebrate Survival Journal
Volume13
Stato di pubblicazionePublished - 2016

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actinium
neurotoxins
Anthozoa
erythrocytes
toxins
sodium channels
extracts
niches
molecular weight
acids
dosage
proteins

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

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title = "Evidence of cytotoxic activity against mammalian red blood cell of Na+ channel neurotoxin (AE1) from sea anemone (actinia equina)",
abstract = "The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.",
keywords = "Actinia equina, Ae1 neurotoxin, Animal Science and Zoology, Hemolysin, Na+ channel neurotoxin, Sea anemone",
author = "Matteo Cammarata and Trapani, {Maria Rosa} and Parisi, {Maria Giovanna}",
year = "2016",
language = "English",
volume = "13",
pages = "309--314",
journal = "Invertebrate Survival Journal",
issn = "1824-307X",
publisher = "University of Modena and Reggio Emilia",

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TY - JOUR

T1 - Evidence of cytotoxic activity against mammalian red blood cell of Na+ channel neurotoxin (AE1) from sea anemone (actinia equina)

AU - Cammarata, Matteo

AU - Trapani, Maria Rosa

AU - Parisi, Maria Giovanna

PY - 2016

Y1 - 2016

N2 - The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.

AB - The diversification of anthozoan toxins played an important role in the ability to colonize various ecological niches. In this study we evaluated the hemolytic activity of HPLC separated fraction of tentacle extracts of sea anemone Actinia equina. Toxic components from acid tissue tentacle extracts were investigated by size exclusion and reverse phase HPLC to characterize cytolytic molecules. A novel low molecular weight active fraction was sequenced by MALDI TOF analysis and a protein correspondent to 5.4 kDa Sodium channel neurotoxin (Ae1) from A. equina was identified. Synthetic Ae1 was assayed and it showed an hemolytic activity against mammalian erythrocytes in a dose dependent manner. Cytolytic activity in addition to neuro-inhibitory function could be a further property of Ae1 toxin repertoire.

KW - Actinia equina

KW - Ae1 neurotoxin

KW - Animal Science and Zoology

KW - Hemolysin

KW - Na+ channel neurotoxin

KW - Sea anemone

UR - http://hdl.handle.net/10447/208983

UR - http://www.isj.unimo.it/articoli/ISJ433.pdf

M3 - Article

VL - 13

SP - 309

EP - 314

JO - Invertebrate Survival Journal

JF - Invertebrate Survival Journal

SN - 1824-307X

ER -