Evidence for the presence of P2y and P2x receptors with different functions in mouse stomach

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Abstract

To clarify the function of P2 receptor subtypes in mouse stomach, the motor responses to ATP, a,h-methyleneATP (a,h-MeATP), P2Xreceptor agonist, 2-methylthioATP (2-MeSATP), P2Y receptor agonist, and the effects of the desensitisation of P2X receptors with a,h-MeATP and of P2Y receptors with ADPhS were analysed recording the endoluminal pressure from whole-organ. ATP-induced relaxationwas antagonised by suramin, non-selective P2 receptor antagonist, by desensitisation of P2Y receptors with ADPhS, and increased bydesensitisation of P2X receptors with a,h-MeATP. a,h-MeATP produced biphasic responses: relaxation, reduced by P2X- or P2Ydesensitisation, and contraction, almost abolished by P2X desensitisation and potentiated by P2Y desensitisation. 2-MeSATP inducedrelaxation, which was antagonised by P2Y desensitisation and increased by P2X desensitisation. Tetrodotoxin increased the relaxationinduced by purines and deeply antagonised the contraction to a,h-MeATP. Our results suggest that in mouse stomach are present muscularP2Y receptors, subserving relaxation, and neuronal presynaptic P2X receptors, mediating contraction.
Lingua originaleEnglish
pagine (da-a)135-140
Numero di pagine6
RivistaEuropean Journal of Pharmacology
Volume513
Stato di pubblicazionePublished - 2005

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Stomach
Adenosine Triphosphate
Presynaptic Receptors
Suramin
Purines
Tetrodotoxin
Pressure
2-methylthio-ATP

All Science Journal Classification (ASJC) codes

  • Pharmacology

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title = "Evidence for the presence of P2y and P2x receptors with different functions in mouse stomach",
abstract = "To clarify the function of P2 receptor subtypes in mouse stomach, the motor responses to ATP, a,h-methyleneATP (a,h-MeATP), P2Xreceptor agonist, 2-methylthioATP (2-MeSATP), P2Y receptor agonist, and the effects of the desensitisation of P2X receptors with a,h-MeATP and of P2Y receptors with ADPhS were analysed recording the endoluminal pressure from whole-organ. ATP-induced relaxationwas antagonised by suramin, non-selective P2 receptor antagonist, by desensitisation of P2Y receptors with ADPhS, and increased bydesensitisation of P2X receptors with a,h-MeATP. a,h-MeATP produced biphasic responses: relaxation, reduced by P2X- or P2Ydesensitisation, and contraction, almost abolished by P2X desensitisation and potentiated by P2Y desensitisation. 2-MeSATP inducedrelaxation, which was antagonised by P2Y desensitisation and increased by P2X desensitisation. Tetrodotoxin increased the relaxationinduced by purines and deeply antagonised the contraction to a,h-MeATP. Our results suggest that in mouse stomach are present muscularP2Y receptors, subserving relaxation, and neuronal presynaptic P2X receptors, mediating contraction.",
author = "Serio, {Rosa Maria} and Flavia Mule'",
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language = "English",
volume = "513",
pages = "135--140",
journal = "European Journal of Pharmacology",
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T1 - Evidence for the presence of P2y and P2x receptors with different functions in mouse stomach

AU - Serio, Rosa Maria

AU - Mule', Flavia

PY - 2005

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N2 - To clarify the function of P2 receptor subtypes in mouse stomach, the motor responses to ATP, a,h-methyleneATP (a,h-MeATP), P2Xreceptor agonist, 2-methylthioATP (2-MeSATP), P2Y receptor agonist, and the effects of the desensitisation of P2X receptors with a,h-MeATP and of P2Y receptors with ADPhS were analysed recording the endoluminal pressure from whole-organ. ATP-induced relaxationwas antagonised by suramin, non-selective P2 receptor antagonist, by desensitisation of P2Y receptors with ADPhS, and increased bydesensitisation of P2X receptors with a,h-MeATP. a,h-MeATP produced biphasic responses: relaxation, reduced by P2X- or P2Ydesensitisation, and contraction, almost abolished by P2X desensitisation and potentiated by P2Y desensitisation. 2-MeSATP inducedrelaxation, which was antagonised by P2Y desensitisation and increased by P2X desensitisation. Tetrodotoxin increased the relaxationinduced by purines and deeply antagonised the contraction to a,h-MeATP. Our results suggest that in mouse stomach are present muscularP2Y receptors, subserving relaxation, and neuronal presynaptic P2X receptors, mediating contraction.

AB - To clarify the function of P2 receptor subtypes in mouse stomach, the motor responses to ATP, a,h-methyleneATP (a,h-MeATP), P2Xreceptor agonist, 2-methylthioATP (2-MeSATP), P2Y receptor agonist, and the effects of the desensitisation of P2X receptors with a,h-MeATP and of P2Y receptors with ADPhS were analysed recording the endoluminal pressure from whole-organ. ATP-induced relaxationwas antagonised by suramin, non-selective P2 receptor antagonist, by desensitisation of P2Y receptors with ADPhS, and increased bydesensitisation of P2X receptors with a,h-MeATP. a,h-MeATP produced biphasic responses: relaxation, reduced by P2X- or P2Ydesensitisation, and contraction, almost abolished by P2X desensitisation and potentiated by P2Y desensitisation. 2-MeSATP inducedrelaxation, which was antagonised by P2Y desensitisation and increased by P2X desensitisation. Tetrodotoxin increased the relaxationinduced by purines and deeply antagonised the contraction to a,h-MeATP. Our results suggest that in mouse stomach are present muscularP2Y receptors, subserving relaxation, and neuronal presynaptic P2X receptors, mediating contraction.

UR - http://hdl.handle.net/10447/5191

M3 - Article

VL - 513

SP - 135

EP - 140

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

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