Abstract
In this paper we demonstrate the existence of a cytoplasmic processing step, never before described, involving both the pre-ribosomal subunits in the sea urchin Paracentrotus lividus. Northern-blot hybridization, primer extension, S1 mapping experiments and in situ hybridizations allowed us to demonstrate that cytoplasmic processed particles are successively re-imported into the nucleus where maturation of their RNAs is completed prior to being exported to the cytoplasm. Our findings lead to the proposal of a new model of ribosome maturation and shuttling.
Lingua originale | English |
---|---|
pagine (da-a) | 1871-1879 |
Numero di pagine | 9 |
Rivista | Cellular and Molecular Life Sciences |
Stato di pubblicazione | Published - 2010 |
All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Molecular Biology
- Pharmacology
- Cellular and Molecular Neuroscience
- Cell Biology