Evaluation of waterborne exposure to heavy metals in innate immune defences present on skin mucus of gilthead seabream (Sparus aurata)

Maria Dioguardi, Matteo Cammarata, Maria Rosa Trapani, Maria Giovanna Parisi, María A. Esteban, Francisco A. Guardiola, José Meseguer, Alberto Cuesta

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41 Citazioni (Scopus)

Abstract

Aquatic animals are continuously exposed to chemical pollutants but the effects evoked in skin surfaces,which receive the most direct contact with them, are poorly investigated. Terminal carbohydratecomposition and immunological components present in skin mucus of gilthead seabream (Sparusaurata L.) specimens exposed to waterborne sublethal dosages of heavy metals [arsenic (As2O3),cadmium (CdCl2) and mercury (CH3HgCl) at 5, 5 and 0.04 mM, respectively for 2, 10 and 30 days wereanalysed. Moreover, the presence of a fucose binding lectin (FBL) was evaluated by western blot and theprotein profiles were by SDS-PAGE and HPLC. Results showed little effects of heavy metals in thepresence of several terminal carbohydrates with few increments or decrements. Most of the enzymeactivities related to immune responses were increased upon heavy metal exposure in the skin mucusincluding bactericidal activity. Methylmercury produced the most dramatic changes increasing all theactivities. Moreover, the FBL was undetected in any of the control fish skin mucus but was evident in allthe heavy metal exposed fish. In addition, As and Cd produced a clear change in the protein profile asevidenced by the lack of a protein band of around 12 kDa which is absent. These protein changes weremore evident with the HPLC study showing the presence of different peaks and differences in intensity.The present results could be useful for better understanding the role and their behaviour of the mucosalimmunity in skin as a key component of the innate immune system against pollutants.
Lingua originaleEnglish
Numero di pagine12
RivistaFISH AND SHELLFISH IMMUNOLOGY
Stato di pubblicazionePublished - 2015

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

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