Evaluation of waterborne exposure to heavy metals in innate immune defences present on skin mucus of gilthead seabream (Sparus aurata)

Maria Dioguardi, Maria Rosa Trapani, Matteo Cammarata, Maria Giovanna Parisi, María A. Esteban, Francisco A. Guardiola, José Meseguer, Alberto Cuesta

Risultato della ricerca: Article

35 Citazioni (Scopus)

Abstract

Aquatic animals are continuously exposed to chemical pollutants but the effects evoked in skin surfaces,which receive the most direct contact with them, are poorly investigated. Terminal carbohydratecomposition and immunological components present in skin mucus of gilthead seabream (Sparusaurata L.) specimens exposed to waterborne sublethal dosages of heavy metals [arsenic (As2O3),cadmium (CdCl2) and mercury (CH3HgCl) at 5, 5 and 0.04 mM, respectively for 2, 10 and 30 days wereanalysed. Moreover, the presence of a fucose binding lectin (FBL) was evaluated by western blot and theprotein profiles were by SDS-PAGE and HPLC. Results showed little effects of heavy metals in thepresence of several terminal carbohydrates with few increments or decrements. Most of the enzymeactivities related to immune responses were increased upon heavy metal exposure in the skin mucusincluding bactericidal activity. Methylmercury produced the most dramatic changes increasing all theactivities. Moreover, the FBL was undetected in any of the control fish skin mucus but was evident in allthe heavy metal exposed fish. In addition, As and Cd produced a clear change in the protein profile asevidenced by the lack of a protein band of around 12 kDa which is absent. These protein changes weremore evident with the HPLC study showing the presence of different peaks and differences in intensity.The present results could be useful for better understanding the role and their behaviour of the mucosalimmunity in skin as a key component of the innate immune system against pollutants.
Lingua originaleEnglish
Numero di pagine12
RivistaDefault journal
Stato di pubblicazionePublished - 2015

Fingerprint

Sea Bream
Sparus aurata
mucus
Mucus
Heavy Metals
skin (animal)
skin
Skin
heavy metals
heavy metal
fucose
lectins
pollutants
Fish
protein
dermal exposure
Fishes
methylmercury compounds
High Pressure Liquid Chromatography
proteins

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cita questo

@article{c2d6a95c369b431f841debb64831389b,
title = "Evaluation of waterborne exposure to heavy metals in innate immune defences present on skin mucus of gilthead seabream (Sparus aurata)",
abstract = "Aquatic animals are continuously exposed to chemical pollutants but the effects evoked in skin surfaces,which receive the most direct contact with them, are poorly investigated. Terminal carbohydratecomposition and immunological components present in skin mucus of gilthead seabream (Sparusaurata L.) specimens exposed to waterborne sublethal dosages of heavy metals [arsenic (As2O3),cadmium (CdCl2) and mercury (CH3HgCl) at 5, 5 and 0.04 mM, respectively for 2, 10 and 30 days wereanalysed. Moreover, the presence of a fucose binding lectin (FBL) was evaluated by western blot and theprotein profiles were by SDS-PAGE and HPLC. Results showed little effects of heavy metals in thepresence of several terminal carbohydrates with few increments or decrements. Most of the enzymeactivities related to immune responses were increased upon heavy metal exposure in the skin mucusincluding bactericidal activity. Methylmercury produced the most dramatic changes increasing all theactivities. Moreover, the FBL was undetected in any of the control fish skin mucus but was evident in allthe heavy metal exposed fish. In addition, As and Cd produced a clear change in the protein profile asevidenced by the lack of a protein band of around 12 kDa which is absent. These protein changes weremore evident with the HPLC study showing the presence of different peaks and differences in intensity.The present results could be useful for better understanding the role and their behaviour of the mucosalimmunity in skin as a key component of the innate immune system against pollutants.",
author = "Maria Dioguardi and Trapani, {Maria Rosa} and Matteo Cammarata and Parisi, {Maria Giovanna} and Esteban, {Mar{\'i}a A.} and Guardiola, {Francisco A.} and Jos{\'e} Meseguer and Alberto Cuesta",
year = "2015",
language = "English",
journal = "Default journal",

}

TY - JOUR

T1 - Evaluation of waterborne exposure to heavy metals in innate immune defences present on skin mucus of gilthead seabream (Sparus aurata)

AU - Dioguardi, Maria

AU - Trapani, Maria Rosa

AU - Cammarata, Matteo

AU - Parisi, Maria Giovanna

AU - Esteban, María A.

AU - Guardiola, Francisco A.

AU - Meseguer, José

AU - Cuesta, Alberto

PY - 2015

Y1 - 2015

N2 - Aquatic animals are continuously exposed to chemical pollutants but the effects evoked in skin surfaces,which receive the most direct contact with them, are poorly investigated. Terminal carbohydratecomposition and immunological components present in skin mucus of gilthead seabream (Sparusaurata L.) specimens exposed to waterborne sublethal dosages of heavy metals [arsenic (As2O3),cadmium (CdCl2) and mercury (CH3HgCl) at 5, 5 and 0.04 mM, respectively for 2, 10 and 30 days wereanalysed. Moreover, the presence of a fucose binding lectin (FBL) was evaluated by western blot and theprotein profiles were by SDS-PAGE and HPLC. Results showed little effects of heavy metals in thepresence of several terminal carbohydrates with few increments or decrements. Most of the enzymeactivities related to immune responses were increased upon heavy metal exposure in the skin mucusincluding bactericidal activity. Methylmercury produced the most dramatic changes increasing all theactivities. Moreover, the FBL was undetected in any of the control fish skin mucus but was evident in allthe heavy metal exposed fish. In addition, As and Cd produced a clear change in the protein profile asevidenced by the lack of a protein band of around 12 kDa which is absent. These protein changes weremore evident with the HPLC study showing the presence of different peaks and differences in intensity.The present results could be useful for better understanding the role and their behaviour of the mucosalimmunity in skin as a key component of the innate immune system against pollutants.

AB - Aquatic animals are continuously exposed to chemical pollutants but the effects evoked in skin surfaces,which receive the most direct contact with them, are poorly investigated. Terminal carbohydratecomposition and immunological components present in skin mucus of gilthead seabream (Sparusaurata L.) specimens exposed to waterborne sublethal dosages of heavy metals [arsenic (As2O3),cadmium (CdCl2) and mercury (CH3HgCl) at 5, 5 and 0.04 mM, respectively for 2, 10 and 30 days wereanalysed. Moreover, the presence of a fucose binding lectin (FBL) was evaluated by western blot and theprotein profiles were by SDS-PAGE and HPLC. Results showed little effects of heavy metals in thepresence of several terminal carbohydrates with few increments or decrements. Most of the enzymeactivities related to immune responses were increased upon heavy metal exposure in the skin mucusincluding bactericidal activity. Methylmercury produced the most dramatic changes increasing all theactivities. Moreover, the FBL was undetected in any of the control fish skin mucus but was evident in allthe heavy metal exposed fish. In addition, As and Cd produced a clear change in the protein profile asevidenced by the lack of a protein band of around 12 kDa which is absent. These protein changes weremore evident with the HPLC study showing the presence of different peaks and differences in intensity.The present results could be useful for better understanding the role and their behaviour of the mucosalimmunity in skin as a key component of the innate immune system against pollutants.

UR - http://hdl.handle.net/10447/129241

M3 - Article

JO - Default journal

JF - Default journal

ER -