Evaluating Resistance to Bt Toxin Cry1Ab by F2 Screen in European Populations of Ostrinia nubilalis (Lepidoptera: Crambidae)

Barbara Rosy Ines Manachini, Stodola, Schuphan, Andow, Micoud, Engels, Brazier, Mottet, Bourguet, Cagáň

Risultato della ricerca: Article

11 Citazioni (Scopus)

Abstract

The large-scale cultivation of transgenic crops producing Bacillus thuringiensis (Bt) toxins have already lead to the evolution of Bt resistance in some pest populations targeted by these crops. We used the F2 screening method for further estimating the frequency of resistance alleles of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), to Bt maize, Zea mays L., producing the CrylAb toxin. In France, Germany, and Italy, 784, 455, and 80 lines of European corn borer were screened for resistance to Mon810 maize, respectively. In Slovakia, 26 lines were screened for resistance to the CrylAb toxin. The cost of F2 screen performed in the four countries varied from US$300 to $1,300 per line screened. The major difference in cost was mostly due to a severe loss of univoltine lines during the screen in Germany and Slovakia. In none of the screened lines did we detect alleles conferring resistance to Mon810 maize or to the CrylAb toxin. The frequency of resistance alleles were <1.0 × 10-3, <1.6 × 10-3, <9.2 × 10-3, and <2.6 × 10-2 in France, Germany, Italy, and Slovakia, with 95% probability, respectively. The average detection probability over all lines was ≈90%. Making the assumption that European corn borer populations in these countries belong to the same genetic entity, the frequency of alleles conferring resistance to the CrylAb produced by the Mon810 maize in western and central Europe was 1.0 × 10-4, with a 95% confidence interval of 0–3.0 × 10-4.
Lingua originaleEnglish
pagine (da-a)1803-1809
Numero di pagine7
RivistaJournal of Economic Entomology
Volume103
Stato di pubblicazionePublished - 2010

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Zearalenone
Bacillus thuringiensis
Crambidae
Lepidoptera
Ostrinia nubilalis
toxin
Zea mays
toxins
Slovakia
maize
corn
Germany
alleles
allele
Population
France
Italy
Gene Frequency
pest resistance
crops

All Science Journal Classification (ASJC) codes

  • Ecology
  • Insect Science

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Evaluating Resistance to Bt Toxin Cry1Ab by F2 Screen in European Populations of Ostrinia nubilalis (Lepidoptera: Crambidae). / Manachini, Barbara Rosy Ines; Stodola; Schuphan; Andow; Micoud; Engels; Brazier; Mottet; Bourguet; Cagáň.

In: Journal of Economic Entomology, Vol. 103, 2010, pag. 1803-1809.

Risultato della ricerca: Article

Manachini, BRI, Stodola, Schuphan, Andow, Micoud, Engels, Brazier, Mottet, Bourguet & Cagáň 2010, 'Evaluating Resistance to Bt Toxin Cry1Ab by F2 Screen in European Populations of Ostrinia nubilalis (Lepidoptera: Crambidae)', Journal of Economic Entomology, vol. 103, pagg. 1803-1809.
Manachini, Barbara Rosy Ines ; Stodola ; Schuphan ; Andow ; Micoud ; Engels ; Brazier ; Mottet ; Bourguet ; Cagáň. / Evaluating Resistance to Bt Toxin Cry1Ab by F2 Screen in European Populations of Ostrinia nubilalis (Lepidoptera: Crambidae). In: Journal of Economic Entomology. 2010 ; Vol. 103. pagg. 1803-1809.
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T1 - Evaluating Resistance to Bt Toxin Cry1Ab by F2 Screen in European Populations of Ostrinia nubilalis (Lepidoptera: Crambidae)

AU - Manachini, Barbara Rosy Ines

AU - Stodola, null

AU - Schuphan, null

AU - Andow, null

AU - Micoud, null

AU - Engels, null

AU - Brazier, null

AU - Mottet, null

AU - Bourguet, null

AU - Cagáň, null

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N2 - The large-scale cultivation of transgenic crops producing Bacillus thuringiensis (Bt) toxins have already lead to the evolution of Bt resistance in some pest populations targeted by these crops. We used the F2 screening method for further estimating the frequency of resistance alleles of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), to Bt maize, Zea mays L., producing the CrylAb toxin. In France, Germany, and Italy, 784, 455, and 80 lines of European corn borer were screened for resistance to Mon810 maize, respectively. In Slovakia, 26 lines were screened for resistance to the CrylAb toxin. The cost of F2 screen performed in the four countries varied from US$300 to $1,300 per line screened. The major difference in cost was mostly due to a severe loss of univoltine lines during the screen in Germany and Slovakia. In none of the screened lines did we detect alleles conferring resistance to Mon810 maize or to the CrylAb toxin. The frequency of resistance alleles were <1.0 × 10-3, <1.6 × 10-3, <9.2 × 10-3, and <2.6 × 10-2 in France, Germany, Italy, and Slovakia, with 95% probability, respectively. The average detection probability over all lines was ≈90%. Making the assumption that European corn borer populations in these countries belong to the same genetic entity, the frequency of alleles conferring resistance to the CrylAb produced by the Mon810 maize in western and central Europe was 1.0 × 10-4, with a 95% confidence interval of 0–3.0 × 10-4.

AB - The large-scale cultivation of transgenic crops producing Bacillus thuringiensis (Bt) toxins have already lead to the evolution of Bt resistance in some pest populations targeted by these crops. We used the F2 screening method for further estimating the frequency of resistance alleles of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), to Bt maize, Zea mays L., producing the CrylAb toxin. In France, Germany, and Italy, 784, 455, and 80 lines of European corn borer were screened for resistance to Mon810 maize, respectively. In Slovakia, 26 lines were screened for resistance to the CrylAb toxin. The cost of F2 screen performed in the four countries varied from US$300 to $1,300 per line screened. The major difference in cost was mostly due to a severe loss of univoltine lines during the screen in Germany and Slovakia. In none of the screened lines did we detect alleles conferring resistance to Mon810 maize or to the CrylAb toxin. The frequency of resistance alleles were <1.0 × 10-3, <1.6 × 10-3, <9.2 × 10-3, and <2.6 × 10-2 in France, Germany, Italy, and Slovakia, with 95% probability, respectively. The average detection probability over all lines was ≈90%. Making the assumption that European corn borer populations in these countries belong to the same genetic entity, the frequency of alleles conferring resistance to the CrylAb produced by the Mon810 maize in western and central Europe was 1.0 × 10-4, with a 95% confidence interval of 0–3.0 × 10-4.

KW - European corn borer, Bt maize, Mon810, resistance management, HDR strategy

UR - http://hdl.handle.net/10447/51363

M3 - Article

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SP - 1803

EP - 1809

JO - Journal of Economic Entomology

JF - Journal of Economic Entomology

SN - 0022-0493

ER -