Ethanol Controls the Self-Assembly and Mesoscopic Properties of Human Insulin Amyloid Spherulites

Federica Piccirilli, Valeria Vetri, Gianpiero Buscarino, Alessio Zaccone, Vito Foderà, Bente Vestergaard, Johannes Krausser, Urszula Łapińska

Risultato della ricerca: Articlepeer review

14 Citazioni (Scopus)


Protein self-assembly into amyloid fibrils or highly hierarchical superstructures is closely linked to neurodegenerative pathologies as Alzheimer's and Parkinson's diseases. Moreover, protein assemblies also emerged as building blocks for bioinspired nanostructured materials. In both the above mentioned fields, the main challenge is to control the growth and properties of the final protein structure. This relies on a more fundamental understanding of how interactions between proteins can determine structures and functions of biomolecular aggregates. Here, we identify a striking effect of the hydration of the single human insulin molecule and solvent properties in controlling hydrophobicity/hydrophilicity, structures, and morphologies of a superstructure named spherulite, observed in connection to Alzheimer's disease. Depending on the presence of ethanol, such structures can incorporate fluorescent molecules with different physicochemical features and span a range of mechanical properties and morphologies. A theoretical model providing a thorough comprehension of the experimental data is developed, highlighting a direct connection between the intimate physical protein-protein interactions, the growth, and the properties of the self-assembled superstructures. Our findings indicate structural variability as a general property for amyloid-like aggregates and not limited to fibrils. This knowledge is pivotal not only for developing effective strategies against pathological amyloids but also for providing a platform to design highly tunable biomaterials, alternative to elongated protein fibrils.
Lingua originaleEnglish
pagine (da-a)3101-3112
Numero di pagine12
Stato di pubblicazionePublished - 2018

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.1600.1606???
  • ???subjectarea.asjc.2500.2508???
  • ???subjectarea.asjc.2500.2505???


Entra nei temi di ricerca di 'Ethanol Controls the Self-Assembly and Mesoscopic Properties of Human Insulin Amyloid Spherulites'. Insieme formano una fingerprint unica.

Cita questo