Estradiol Decreases Xanthine Dehydrogenase Enzyme Activity and Protein Expression in Non-Tumorigenic and Malignant Human Mammary Epithelial Cells

Concetta Nicotra, Gennaro Taibi, Letizia Cocciadiferro, Giuseppe Carruba, Vitale Miceli

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    3 Citazioni (Scopus)

    Abstract

    The retinoic acid deficiency in breast tumour epithelial cells has been ascribed to an insufficient expression of either the enzyme(s) involved in its biosynthesis or the cellular retinol binding protein (CRBP) or both. In an attempt to define the mechanisms underpinning retinoic acid deficiency in these cell model systems, we have investigated the potential regulatory effect of oestrogen (17b-estradiol) on one key player in retinoic acid biosynthesis, the xanthine dehydrogenase (XDH). This enzyme is consistently expressed and very active in non-malignant human mammary epithelial cells (HMEC), as opposed to tumour MDA-MB231 and MCF7 cells. In these latter two cell lines, as opposed to HMEC cells, we observe a residual ability of XDH to produce retinoic acid from retinaldehyde and the inability to use retinol, as a consequence of a deficit in CRBP. In addition, estradiol treatment of MDA-MB231 and MCF7 cells decreases protein expression and activity of the enzyme, with no modification of the mRNA transcript levels, eventually leading to deteriorate further retinoic acid production.
    Lingua originaleEnglish
    pagine (da-a)688-692
    Numero di pagine5
    RivistaJournal of Cellular Biochemistry
    Volume2009-10
    Stato di pubblicazionePublished - 2009

    Fingerprint

    Xanthine Dehydrogenase
    Enzyme activity
    Tretinoin
    Estradiol
    Breast
    Epithelial Cells
    Cellular Retinol-Binding Proteins
    Enzymes
    Biosynthesis
    MCF-7 Cells
    Proteins
    Tumors
    Retinaldehyde
    Vitamin A
    Estrogens
    Cells
    Breast Neoplasms
    Cell Line
    Messenger RNA
    Neoplasms

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Cita questo

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    title = "Estradiol Decreases Xanthine Dehydrogenase Enzyme Activity and Protein Expression in Non-Tumorigenic and Malignant Human Mammary Epithelial Cells",
    abstract = "The retinoic acid deficiency in breast tumour epithelial cells has been ascribed to an insufficient expression of either the enzyme(s) involved in its biosynthesis or the cellular retinol binding protein (CRBP) or both. In an attempt to define the mechanisms underpinning retinoic acid deficiency in these cell model systems, we have investigated the potential regulatory effect of oestrogen (17b-estradiol) on one key player in retinoic acid biosynthesis, the xanthine dehydrogenase (XDH). This enzyme is consistently expressed and very active in non-malignant human mammary epithelial cells (HMEC), as opposed to tumour MDA-MB231 and MCF7 cells. In these latter two cell lines, as opposed to HMEC cells, we observe a residual ability of XDH to produce retinoic acid from retinaldehyde and the inability to use retinol, as a consequence of a deficit in CRBP. In addition, estradiol treatment of MDA-MB231 and MCF7 cells decreases protein expression and activity of the enzyme, with no modification of the mRNA transcript levels, eventually leading to deteriorate further retinoic acid production.",
    keywords = "RETINOIC ACID; XANTHINE DEHYDROGENASE; XANTHINE OXIDASE; ESTRADIOL",
    author = "Concetta Nicotra and Gennaro Taibi and Letizia Cocciadiferro and Giuseppe Carruba and Vitale Miceli",
    year = "2009",
    language = "English",
    volume = "2009-10",
    pages = "688--692",
    journal = "Journal of Cellular Biochemistry",
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    TY - JOUR

    T1 - Estradiol Decreases Xanthine Dehydrogenase Enzyme Activity and Protein Expression in Non-Tumorigenic and Malignant Human Mammary Epithelial Cells

    AU - Nicotra, Concetta

    AU - Taibi, Gennaro

    AU - Cocciadiferro, Letizia

    AU - Carruba, Giuseppe

    AU - Miceli, Vitale

    PY - 2009

    Y1 - 2009

    N2 - The retinoic acid deficiency in breast tumour epithelial cells has been ascribed to an insufficient expression of either the enzyme(s) involved in its biosynthesis or the cellular retinol binding protein (CRBP) or both. In an attempt to define the mechanisms underpinning retinoic acid deficiency in these cell model systems, we have investigated the potential regulatory effect of oestrogen (17b-estradiol) on one key player in retinoic acid biosynthesis, the xanthine dehydrogenase (XDH). This enzyme is consistently expressed and very active in non-malignant human mammary epithelial cells (HMEC), as opposed to tumour MDA-MB231 and MCF7 cells. In these latter two cell lines, as opposed to HMEC cells, we observe a residual ability of XDH to produce retinoic acid from retinaldehyde and the inability to use retinol, as a consequence of a deficit in CRBP. In addition, estradiol treatment of MDA-MB231 and MCF7 cells decreases protein expression and activity of the enzyme, with no modification of the mRNA transcript levels, eventually leading to deteriorate further retinoic acid production.

    AB - The retinoic acid deficiency in breast tumour epithelial cells has been ascribed to an insufficient expression of either the enzyme(s) involved in its biosynthesis or the cellular retinol binding protein (CRBP) or both. In an attempt to define the mechanisms underpinning retinoic acid deficiency in these cell model systems, we have investigated the potential regulatory effect of oestrogen (17b-estradiol) on one key player in retinoic acid biosynthesis, the xanthine dehydrogenase (XDH). This enzyme is consistently expressed and very active in non-malignant human mammary epithelial cells (HMEC), as opposed to tumour MDA-MB231 and MCF7 cells. In these latter two cell lines, as opposed to HMEC cells, we observe a residual ability of XDH to produce retinoic acid from retinaldehyde and the inability to use retinol, as a consequence of a deficit in CRBP. In addition, estradiol treatment of MDA-MB231 and MCF7 cells decreases protein expression and activity of the enzyme, with no modification of the mRNA transcript levels, eventually leading to deteriorate further retinoic acid production.

    KW - RETINOIC ACID; XANTHINE DEHYDROGENASE; XANTHINE OXIDASE; ESTRADIOL

    UR - http://hdl.handle.net/10447/50499

    M3 - Article

    VL - 2009-10

    SP - 688

    EP - 692

    JO - Journal of Cellular Biochemistry

    JF - Journal of Cellular Biochemistry

    SN - 0730-2312

    ER -