Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin

Giovanni Grasso, Antonio Morabito, Michael L. Brines, Giovanni Grasso, Battesimo Macrì, Alessandra Sfacteria, Marcello Passalacqua, Francesco Tomasello, Michele Buemi

Risultato della ricerca: Article

50 Citazioni (Scopus)

Abstract

OBJECTIVE: Erythropoietin (EPO) is a pleiotropic cytokine originally identified for its role in erythropoiesis. Recent studies have demonstrated that EPO and its receptor (EPO-R) are expressed in the central nervous system, where EPO exerts neuroprotective functions. Because the expression of the EPO and EPO-R network is poorly investigated in the central nervous system, the aim of the present study was to investigate whether the resident EPO and EPO-R network is activated in the injured nervous system. METHODS: A well-standardized model of compressive spinal cord injury in rats was used. EPO and EPO-R expression was determined by immunohistochemical analysis at 8 hours and at 2, 8, and 14 days in the spinal cord of injured and noninjured rats. RESULTS: In noninjured spinal cord, weak immunohistochemical expression of EPO and EPO-R was observed in neuronal and glial cells as well as in endothelial and ependymal cells. In injured rats, a marked increase of expression of EPO and EPO-R was observed in neurons, vascular endothelium, and glial cells at 8 hours after injury, peaking at 8 days, after which it gradually decreased. Two weeks after injury, EPO immunoreactivity was scarcely detected in neurons, whereas glial cells and vascular endothelium expressed strong EPO-R immunoreactivity. CONCLUSION: These observations suggest that the local EPO and EPO-R system is markedly engaged in the early stages after nervous tissue injury. The reduction in EPO immunoexpression and the increase in EPO-R staining strongly support the possible usefulness of a therapeutic approach based on exogenous EPO administration.
Lingua originaleEnglish
pagine (da-a)821-827
RivistaNEUROSURGERY
Volume56 (4)
Stato di pubblicazionePublished - 2005

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Erythropoietin Receptors
Erythropoietin
Spinal Cord Injuries
Therapeutics
Neuroglia
Vascular Endothelium
Spinal Cord
Wounds and Injuries
Central Nervous System
Neurons

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Cita questo

Grasso, G., Morabito, A., Brines, M. L., Grasso, G., Macrì, B., Sfacteria, A., ... Buemi, M. (2005). Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin. NEUROSURGERY, 56 (4), 821-827.

Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin. / Grasso, Giovanni; Morabito, Antonio; Brines, Michael L.; Grasso, Giovanni; Macrì, Battesimo; Sfacteria, Alessandra; Passalacqua, Marcello; Tomasello, Francesco; Buemi, Michele.

In: NEUROSURGERY, Vol. 56 (4), 2005, pag. 821-827.

Risultato della ricerca: Article

Grasso, G, Morabito, A, Brines, ML, Grasso, G, Macrì, B, Sfacteria, A, Passalacqua, M, Tomasello, F & Buemi, M 2005, 'Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin', NEUROSURGERY, vol. 56 (4), pagg. 821-827.
Grasso, Giovanni ; Morabito, Antonio ; Brines, Michael L. ; Grasso, Giovanni ; Macrì, Battesimo ; Sfacteria, Alessandra ; Passalacqua, Marcello ; Tomasello, Francesco ; Buemi, Michele. / Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin. In: NEUROSURGERY. 2005 ; Vol. 56 (4). pagg. 821-827.
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title = "Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin",
abstract = "OBJECTIVE: Erythropoietin (EPO) is a pleiotropic cytokine originally identified for its role in erythropoiesis. Recent studies have demonstrated that EPO and its receptor (EPO-R) are expressed in the central nervous system, where EPO exerts neuroprotective functions. Because the expression of the EPO and EPO-R network is poorly investigated in the central nervous system, the aim of the present study was to investigate whether the resident EPO and EPO-R network is activated in the injured nervous system. METHODS: A well-standardized model of compressive spinal cord injury in rats was used. EPO and EPO-R expression was determined by immunohistochemical analysis at 8 hours and at 2, 8, and 14 days in the spinal cord of injured and noninjured rats. RESULTS: In noninjured spinal cord, weak immunohistochemical expression of EPO and EPO-R was observed in neuronal and glial cells as well as in endothelial and ependymal cells. In injured rats, a marked increase of expression of EPO and EPO-R was observed in neurons, vascular endothelium, and glial cells at 8 hours after injury, peaking at 8 days, after which it gradually decreased. Two weeks after injury, EPO immunoreactivity was scarcely detected in neurons, whereas glial cells and vascular endothelium expressed strong EPO-R immunoreactivity. CONCLUSION: These observations suggest that the local EPO and EPO-R system is markedly engaged in the early stages after nervous tissue injury. The reduction in EPO immunoexpression and the increase in EPO-R staining strongly support the possible usefulness of a therapeutic approach based on exogenous EPO administration.",
keywords = "Erythropoietin, Erythropoietin receptor, Neurology (clinical), Neuroprotection, Spinal cord injury, Surgery",
author = "Giovanni Grasso and Antonio Morabito and Brines, {Michael L.} and Giovanni Grasso and Battesimo Macr{\`i} and Alessandra Sfacteria and Marcello Passalacqua and Francesco Tomasello and Michele Buemi",
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TY - JOUR

T1 - Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin

AU - Grasso, Giovanni

AU - Morabito, Antonio

AU - Brines, Michael L.

AU - Grasso, Giovanni

AU - Macrì, Battesimo

AU - Sfacteria, Alessandra

AU - Passalacqua, Marcello

AU - Tomasello, Francesco

AU - Buemi, Michele

PY - 2005

Y1 - 2005

N2 - OBJECTIVE: Erythropoietin (EPO) is a pleiotropic cytokine originally identified for its role in erythropoiesis. Recent studies have demonstrated that EPO and its receptor (EPO-R) are expressed in the central nervous system, where EPO exerts neuroprotective functions. Because the expression of the EPO and EPO-R network is poorly investigated in the central nervous system, the aim of the present study was to investigate whether the resident EPO and EPO-R network is activated in the injured nervous system. METHODS: A well-standardized model of compressive spinal cord injury in rats was used. EPO and EPO-R expression was determined by immunohistochemical analysis at 8 hours and at 2, 8, and 14 days in the spinal cord of injured and noninjured rats. RESULTS: In noninjured spinal cord, weak immunohistochemical expression of EPO and EPO-R was observed in neuronal and glial cells as well as in endothelial and ependymal cells. In injured rats, a marked increase of expression of EPO and EPO-R was observed in neurons, vascular endothelium, and glial cells at 8 hours after injury, peaking at 8 days, after which it gradually decreased. Two weeks after injury, EPO immunoreactivity was scarcely detected in neurons, whereas glial cells and vascular endothelium expressed strong EPO-R immunoreactivity. CONCLUSION: These observations suggest that the local EPO and EPO-R system is markedly engaged in the early stages after nervous tissue injury. The reduction in EPO immunoexpression and the increase in EPO-R staining strongly support the possible usefulness of a therapeutic approach based on exogenous EPO administration.

AB - OBJECTIVE: Erythropoietin (EPO) is a pleiotropic cytokine originally identified for its role in erythropoiesis. Recent studies have demonstrated that EPO and its receptor (EPO-R) are expressed in the central nervous system, where EPO exerts neuroprotective functions. Because the expression of the EPO and EPO-R network is poorly investigated in the central nervous system, the aim of the present study was to investigate whether the resident EPO and EPO-R network is activated in the injured nervous system. METHODS: A well-standardized model of compressive spinal cord injury in rats was used. EPO and EPO-R expression was determined by immunohistochemical analysis at 8 hours and at 2, 8, and 14 days in the spinal cord of injured and noninjured rats. RESULTS: In noninjured spinal cord, weak immunohistochemical expression of EPO and EPO-R was observed in neuronal and glial cells as well as in endothelial and ependymal cells. In injured rats, a marked increase of expression of EPO and EPO-R was observed in neurons, vascular endothelium, and glial cells at 8 hours after injury, peaking at 8 days, after which it gradually decreased. Two weeks after injury, EPO immunoreactivity was scarcely detected in neurons, whereas glial cells and vascular endothelium expressed strong EPO-R immunoreactivity. CONCLUSION: These observations suggest that the local EPO and EPO-R system is markedly engaged in the early stages after nervous tissue injury. The reduction in EPO immunoexpression and the increase in EPO-R staining strongly support the possible usefulness of a therapeutic approach based on exogenous EPO administration.

KW - Erythropoietin

KW - Erythropoietin receptor

KW - Neurology (clinical)

KW - Neuroprotection

KW - Spinal cord injury

KW - Surgery

UR - http://hdl.handle.net/10447/24930

M3 - Article

VL - 56 (4)

SP - 821

EP - 827

JO - NEUROSURGERY

JF - NEUROSURGERY

SN - 0148-396X

ER -