Enhanced In Situ Availability of Aphanizomenon Flos-Aquae Constituents Entrapped in Buccal Films for the Treatment of Oxidative Stress-Related Oral Diseases: Biomechanical Characterization and In Vitro/Ex Vivo Evaluation

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Abstract

In recent years, the key role of oxidative stress in pathogenesis of oral diseases has beenemphasized and the use of antioxidant agents has been encouraged. Aphanizomenon flos-aquae (AFA) isa unicellular blue-green alga with antioxidant and anti-inflammatory properties. The aim of this studywas the formulation and characterization of mucoadhesive thin layer films loaded with AFA, finalizedto the treatment of oxidative stress (OS)-related oral diseases. First, to enhance the bioavailabilityof AFA constituents, the raw food grade material was appropriately treated by a high frequencyhomogenization able to disrupt cell walls. Thus, Eudragit® E100-based buccal films were producedby the solvent casting method, containing 7% and 18% of AFA. The films, characterized by uniformityin thickness, weight, and drug content, showed low swelling degree, good muco-adhesiveness andcontrolled drug release. The mechanical tests showed elastic moduli of films of almost 5 MPa that iswell-suitable for human buccal applications without discomfort, besides biaxial tests highlighted amarked material isotropy. Permeation studies through porcine mucosae demonstrated the ability offilms to promote AFA penetration in the tissues, and when sublingually administered, they produceda drug flux up to six-fold higher than an AFA solution. The new formulations represent an interestingalternative for the development of cosmetics and nutraceuticals with a functional appeal containingplant extracts.
Lingua originaleEnglish
pagine (da-a)35-
Numero di pagine23
RivistaDefault journal
Volume11
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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title = "Enhanced In Situ Availability of Aphanizomenon Flos-Aquae Constituents Entrapped in Buccal Films for the Treatment of Oxidative Stress-Related Oral Diseases: Biomechanical Characterization and In Vitro/Ex Vivo Evaluation",
abstract = "In recent years, the key role of oxidative stress in pathogenesis of oral diseases has beenemphasized and the use of antioxidant agents has been encouraged. Aphanizomenon flos-aquae (AFA) isa unicellular blue-green alga with antioxidant and anti-inflammatory properties. The aim of this studywas the formulation and characterization of mucoadhesive thin layer films loaded with AFA, finalizedto the treatment of oxidative stress (OS)-related oral diseases. First, to enhance the bioavailabilityof AFA constituents, the raw food grade material was appropriately treated by a high frequencyhomogenization able to disrupt cell walls. Thus, Eudragit{\circledR} E100-based buccal films were producedby the solvent casting method, containing 7{\%} and 18{\%} of AFA. The films, characterized by uniformityin thickness, weight, and drug content, showed low swelling degree, good muco-adhesiveness andcontrolled drug release. The mechanical tests showed elastic moduli of films of almost 5 MPa that iswell-suitable for human buccal applications without discomfort, besides biaxial tests highlighted amarked material isotropy. Permeation studies through porcine mucosae demonstrated the ability offilms to promote AFA penetration in the tissues, and when sublingually administered, they produceda drug flux up to six-fold higher than an AFA solution. The new formulations represent an interestingalternative for the development of cosmetics and nutraceuticals with a functional appeal containingplant extracts.",
author = "Massimiliano Zingales and Giuseppina Campisi and {De Caro}, Viviana and Denise Murgia and Gioacchino Alotta and Emanuela Bologna and Francesco Seidita",
year = "2019",
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T1 - Enhanced In Situ Availability of Aphanizomenon Flos-Aquae Constituents Entrapped in Buccal Films for the Treatment of Oxidative Stress-Related Oral Diseases: Biomechanical Characterization and In Vitro/Ex Vivo Evaluation

AU - Zingales, Massimiliano

AU - Campisi, Giuseppina

AU - De Caro, Viviana

AU - Murgia, Denise

AU - Alotta, Gioacchino

AU - Bologna, Emanuela

AU - Seidita, Francesco

PY - 2019

Y1 - 2019

N2 - In recent years, the key role of oxidative stress in pathogenesis of oral diseases has beenemphasized and the use of antioxidant agents has been encouraged. Aphanizomenon flos-aquae (AFA) isa unicellular blue-green alga with antioxidant and anti-inflammatory properties. The aim of this studywas the formulation and characterization of mucoadhesive thin layer films loaded with AFA, finalizedto the treatment of oxidative stress (OS)-related oral diseases. First, to enhance the bioavailabilityof AFA constituents, the raw food grade material was appropriately treated by a high frequencyhomogenization able to disrupt cell walls. Thus, Eudragit® E100-based buccal films were producedby the solvent casting method, containing 7% and 18% of AFA. The films, characterized by uniformityin thickness, weight, and drug content, showed low swelling degree, good muco-adhesiveness andcontrolled drug release. The mechanical tests showed elastic moduli of films of almost 5 MPa that iswell-suitable for human buccal applications without discomfort, besides biaxial tests highlighted amarked material isotropy. Permeation studies through porcine mucosae demonstrated the ability offilms to promote AFA penetration in the tissues, and when sublingually administered, they produceda drug flux up to six-fold higher than an AFA solution. The new formulations represent an interestingalternative for the development of cosmetics and nutraceuticals with a functional appeal containingplant extracts.

AB - In recent years, the key role of oxidative stress in pathogenesis of oral diseases has beenemphasized and the use of antioxidant agents has been encouraged. Aphanizomenon flos-aquae (AFA) isa unicellular blue-green alga with antioxidant and anti-inflammatory properties. The aim of this studywas the formulation and characterization of mucoadhesive thin layer films loaded with AFA, finalizedto the treatment of oxidative stress (OS)-related oral diseases. First, to enhance the bioavailabilityof AFA constituents, the raw food grade material was appropriately treated by a high frequencyhomogenization able to disrupt cell walls. Thus, Eudragit® E100-based buccal films were producedby the solvent casting method, containing 7% and 18% of AFA. The films, characterized by uniformityin thickness, weight, and drug content, showed low swelling degree, good muco-adhesiveness andcontrolled drug release. The mechanical tests showed elastic moduli of films of almost 5 MPa that iswell-suitable for human buccal applications without discomfort, besides biaxial tests highlighted amarked material isotropy. Permeation studies through porcine mucosae demonstrated the ability offilms to promote AFA penetration in the tissues, and when sublingually administered, they produceda drug flux up to six-fold higher than an AFA solution. The new formulations represent an interestingalternative for the development of cosmetics and nutraceuticals with a functional appeal containingplant extracts.

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