Cancer stem cells (CSC) have a central role in driving tumor growth. Since metabolism is becoming an important diagnostic and therapeutictarget, characterization of CSC line energetic properties is an emerging need. Embryonic and adult stem cells, compared to differentiated cells,exhibit a reduced mitochondrial activity and a stronger dependence on aerobic glycolysis. Here, we aimed to comparatively analyzebioenergetics features of the human osteosarcoma 3AB‐OS CSC‐like line, and the parental osteosarcoma MG63 cells, from which 3AB‐OS cellshave been previously selected. Our results suggest that 3AB‐OS cells depend on glycolytic metabolism more strongly than MG63 cells. Indeed,growth in glucose shortage or in presence of galactose or pyruvate (mitochondrial specific substrates) leads to a significant reduction of theirproliferation compared to MG63 cells. Accordingly, 3AB‐OS cells show an increased expression of lactate dehydrogenase A (LDHA) and a largeraccumulation of lactate in the culture medium. In line with these findings 3AB‐OS cells as compared to MG63 cells present a reducedmitochondrial respiration, a stronger sensitivity to glucose depletion or glycolysis inhibition and a lessened sensitivity to oxidativephosphorylation inhibitors. Additionally, in contrast to MG63 cells, 3AB‐OS display fragmented mitochondria, which become networked as theygrow in glucose‐rich medium, while almost entirely loose these structures growing in low glucose. Overall, our findings suggest that 3AB‐OS CSCenergy metabolism is more similar to normal stem cells and to cancer cells characterized by a glycolytic anaerobic metabolism.
|Numero di pagine||12|
|Rivista||Journal of Cellular Biochemistry|
|Stato di pubblicazione||Published - 2014|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology