A new role for fat supplements, in particular conjugated linoleic acid (CLA), has been delineated in steroidogenesis,although the underlying molecular mechanisms have not yet been elucidated. The aims of the present study were toidentify the pathway stimulated by CLA supplementation using a cell culture model and to determine whether thissame pathway is also stimulated in vivo by CLA supplementation associated with exercise. In vitro, Leydig tumour ratcells (R2C) supplemented with different concentrations of CLA exhibited increasing testosterone biosynthesisaccompanied by increasing levels of CYP17A1 mRNA and protein. In vivo, trained mice showed an increase in freeplasma testosterone and an up-regulation of CYP17A1 mRNA and protein. The effect of training on CYP17A1expression and testosterone biosynthesis was significantly higher in the trained mice supplemented with CLAcompared to the placebo. The results of the present study demonstrated that CLA stimulates testosteronebiosynthesis via CYP17A1, and endurance training led to the synthesis of testosterone in vivo by inducing theoverexpression of CYP17A1 mRNA and protein in the Leydig cells of the testis. This effect was enhanced by CLAsupplementation. Therefore, CLA-associated physical activity may be used for its steroidogenic property in differentfields, such as alimentary industry, human reproductive medicine, sport science, and anti-muscle wasting.
|Numero di pagine||10|
|Stato di pubblicazione||Published - 2013|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
Farina, F., Cappello, F., Rizzuto, L., Marino Gammazza, A., Barone, R., Di Felice, V., Zummo, G., Morici, G., Stampone, T., Lo Giudice, G., & Marozzi, P. (2013). Endurance Exercise and Conjugated Linoleic Acid (CLA)Supplementation Up-Regulate CYP17A1 and StimulateTestosterone Biosynthesis. PLoS One, 8.