Endophytes from medicinal plants’ seeds: exploring new reservoirs of bioactive molecules

Risultato della ricerca: Other

Abstract

Plant-associated microorganisms have recently gained more attention for their influence on plant health and biotechnological relevance. Bioactive molecules have been already isolated from plant-associated bacteria. Seed-borne bacterial endophytes have not been much explored though. Such endophytes are particularly important, since they can influence germination and be transmitted from generation to generation. Interestingly, seed endophytes from medicinal plants could influence the production of molecules with therapeutic properties. Bacterial endophytic strains were extracted from surface-sterilized seeds of the medicinal plant Echinacea purpurea and their 16S rRNA genes were sequenced. The analysis revealed the predominance of Paenibacillus (55.8%) and Pantoea (39.5%) genera among isolates. Fluorescence in situ hybridization and confocal laser scanning microscopy (FISH–CLSM) revealed a consistent colonization of Echinacea seeds by microbial endophytes as well as epiphytes. Resistance to antibiotics revealed that Ciprofloxacin and Rifampicin are the most effective antibiotics, even at minimum concentrations (respectively 0.5 and 5 mg/l). Isolates were less sensitive to Tetracycline and Streptomycin instead.Further investigation of Echinacea plants seed-borne endophytes may elucidate how such endophytes influence the therapeutic properties of the plant, and pave the way for the discovery of biotechnologically relevant compounds.
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2017

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endophytes
medicinal plants
seeds
Echinacea
medicinal properties
antibiotics
Pantoea
Echinacea purpurea
Paenibacillus
rifampicin
plant health
epiphytes
ciprofloxacin
confocal laser scanning microscopy
streptomycin
Spermatophytina
fluorescence in situ hybridization
tetracycline
ribosomal RNA
germination

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title = "Endophytes from medicinal plants’ seeds: exploring new reservoirs of bioactive molecules",
abstract = "Plant-associated microorganisms have recently gained more attention for their influence on plant health and biotechnological relevance. Bioactive molecules have been already isolated from plant-associated bacteria. Seed-borne bacterial endophytes have not been much explored though. Such endophytes are particularly important, since they can influence germination and be transmitted from generation to generation. Interestingly, seed endophytes from medicinal plants could influence the production of molecules with therapeutic properties. Bacterial endophytic strains were extracted from surface-sterilized seeds of the medicinal plant Echinacea purpurea and their 16S rRNA genes were sequenced. The analysis revealed the predominance of Paenibacillus (55.8{\%}) and Pantoea (39.5{\%}) genera among isolates. Fluorescence in situ hybridization and confocal laser scanning microscopy (FISH–CLSM) revealed a consistent colonization of Echinacea seeds by microbial endophytes as well as epiphytes. Resistance to antibiotics revealed that Ciprofloxacin and Rifampicin are the most effective antibiotics, even at minimum concentrations (respectively 0.5 and 5 mg/l). Isolates were less sensitive to Tetracycline and Streptomycin instead.Further investigation of Echinacea plants seed-borne endophytes may elucidate how such endophytes influence the therapeutic properties of the plant, and pave the way for the discovery of biotechnologically relevant compounds.",
keywords = "Bioactive molecules, Echinacea purpurea, Endophytes, FISH–CLSM",
author = "Puglia, {Anna Maria} and Giuseppe Gallo and Teresa Faddetta",
year = "2017",
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TY - CONF

T1 - Endophytes from medicinal plants’ seeds: exploring new reservoirs of bioactive molecules

AU - Puglia, Anna Maria

AU - Gallo, Giuseppe

AU - Faddetta, Teresa

PY - 2017

Y1 - 2017

N2 - Plant-associated microorganisms have recently gained more attention for their influence on plant health and biotechnological relevance. Bioactive molecules have been already isolated from plant-associated bacteria. Seed-borne bacterial endophytes have not been much explored though. Such endophytes are particularly important, since they can influence germination and be transmitted from generation to generation. Interestingly, seed endophytes from medicinal plants could influence the production of molecules with therapeutic properties. Bacterial endophytic strains were extracted from surface-sterilized seeds of the medicinal plant Echinacea purpurea and their 16S rRNA genes were sequenced. The analysis revealed the predominance of Paenibacillus (55.8%) and Pantoea (39.5%) genera among isolates. Fluorescence in situ hybridization and confocal laser scanning microscopy (FISH–CLSM) revealed a consistent colonization of Echinacea seeds by microbial endophytes as well as epiphytes. Resistance to antibiotics revealed that Ciprofloxacin and Rifampicin are the most effective antibiotics, even at minimum concentrations (respectively 0.5 and 5 mg/l). Isolates were less sensitive to Tetracycline and Streptomycin instead.Further investigation of Echinacea plants seed-borne endophytes may elucidate how such endophytes influence the therapeutic properties of the plant, and pave the way for the discovery of biotechnologically relevant compounds.

AB - Plant-associated microorganisms have recently gained more attention for their influence on plant health and biotechnological relevance. Bioactive molecules have been already isolated from plant-associated bacteria. Seed-borne bacterial endophytes have not been much explored though. Such endophytes are particularly important, since they can influence germination and be transmitted from generation to generation. Interestingly, seed endophytes from medicinal plants could influence the production of molecules with therapeutic properties. Bacterial endophytic strains were extracted from surface-sterilized seeds of the medicinal plant Echinacea purpurea and their 16S rRNA genes were sequenced. The analysis revealed the predominance of Paenibacillus (55.8%) and Pantoea (39.5%) genera among isolates. Fluorescence in situ hybridization and confocal laser scanning microscopy (FISH–CLSM) revealed a consistent colonization of Echinacea seeds by microbial endophytes as well as epiphytes. Resistance to antibiotics revealed that Ciprofloxacin and Rifampicin are the most effective antibiotics, even at minimum concentrations (respectively 0.5 and 5 mg/l). Isolates were less sensitive to Tetracycline and Streptomycin instead.Further investigation of Echinacea plants seed-borne endophytes may elucidate how such endophytes influence the therapeutic properties of the plant, and pave the way for the discovery of biotechnologically relevant compounds.

KW - Bioactive molecules

KW - Echinacea purpurea

KW - Endophytes

KW - FISH–CLSM

UR - http://hdl.handle.net/10447/253691

M3 - Other

ER -