Endocrine and ovarian response after a 2-daycontrolled suckling and eCG treatment inlactating rabbit does

Antonino Di Grigoli, Adriana Bonanno, Gabriele Tornambè, Tornambè, Bonanno, Rebollar, Lorenzo, Di Grigoli

Risultato della ricerca: Article

18 Citazioni (Scopus)

Abstract

Synchronization methods are used to obtain higher fertility when artificial insemination (AI) is applied tolactating rabbit does. The most common methods are eCG administration or temporary doe–litter separation.Nevertheless, drawbacks have been reported, such as negative side effects of hormonal treatment in the doeand low litter growth due to absence of suckling, respectively. Recently, improved reproductive performance(without visible consequences on young rabbit growth), has been obtained by applying a 2-day controllednursing method before AI, by allowing for a 10 min nursing of the litter 24 h of separation. The presentstudy was undertaken to examine the pituitary (PRL, LH, FSH) and the ovarian response (follicle size andnumber) to those methods. A total of 442 lactating does inseminated on day 11 post-partum were distributedin three experimental groups: 2CN (closing of nest box on day 9, controlled nursing on days 10 and 11),eCG (20 IU administered on day 9 post-partum) and CONTROL (untreated). Blood samples were obtainedfrom 10 does per group at 48, 24 and 0 h before AI, and 1 h after AI. Both 2CN and eCG treatments similarlyimproved sexual receptivity (76.3, 77.5 and 58.2%, respectively; P < 0.001) and fertility (63.1, 64.1 and48.4%, respectively; P < 0.05) in lactating does, compared to the CONTROL group. Similar plasma FSHlevels in all groups of does and sampling times were observed. Due to the absence of suckling, plasmaconcentration of PRL on day 10 post-partum in the 2CN group was lower than in the CONTROL group(P < 0.05); this endocrine change in PRL levels could explain the better reproductive performances obtained with 2CN treatment. At 1 h after exogenous administration of GnRH (at the moment of AI) a high LHresponse was observed in all groups (P < 0.001).Ovaries from 20 rabbits treated in the same way but uninseminated (2CN, n = 10; eCG, n = 5; CONTROL,n = 5 does) were obtained on day 11 post-partum in order to check the morphometric status (weight, width andheight) and to make histological and immunohistochemical studies to detect growth hormone receptor (GHR).As a result, synchronization methods did not showany significant difference in relation to the CONTROLgroup. However, a small increase in the number of primary follicles was evidenced in the 2CN group withrespect to the eCG group, similarly to the CONTROL group (23.0±3.7, 9.4±4.9 and 14.8±4.92 primaryfollicles, respectively; P = 0.1). GH-R immunostaining-presence was more evident in the 2CN and the eCGgroups, including primordial follicles and oocytes themselves. Thus, there could have been some direct effectsofGHon follicular development, as described in other species. Some ovarian parameters described open newways to study intra-ovarian mechanism of follicular development in the post-partum period of rabbit does.
Lingua originaleEnglish
pagine (da-a)316-328
Numero di pagine339
RivistaAnimal Reproduction Science
Volume104
Stato di pubblicazionePublished - 2008

Fingerprint

Artificial Insemination
suckling
artificial insemination
rabbits
Rabbits
follicular development
breast feeding
Fertility
reproductive performance
Nursing
Somatotropin Receptors
Therapeutics
methodology
Ovarian Follicle
nest boxes
postpartum period
Growth
Gonadotropin-Releasing Hormone
Oocytes
Ovary

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

Cita questo

@article{8f8282b9f2c14fe48b6c5e17aa255059,
title = "Endocrine and ovarian response after a 2-daycontrolled suckling and eCG treatment inlactating rabbit does",
abstract = "Synchronization methods are used to obtain higher fertility when artificial insemination (AI) is applied tolactating rabbit does. The most common methods are eCG administration or temporary doe–litter separation.Nevertheless, drawbacks have been reported, such as negative side effects of hormonal treatment in the doeand low litter growth due to absence of suckling, respectively. Recently, improved reproductive performance(without visible consequences on young rabbit growth), has been obtained by applying a 2-day controllednursing method before AI, by allowing for a 10 min nursing of the litter 24 h of separation. The presentstudy was undertaken to examine the pituitary (PRL, LH, FSH) and the ovarian response (follicle size andnumber) to those methods. A total of 442 lactating does inseminated on day 11 post-partum were distributedin three experimental groups: 2CN (closing of nest box on day 9, controlled nursing on days 10 and 11),eCG (20 IU administered on day 9 post-partum) and CONTROL (untreated). Blood samples were obtainedfrom 10 does per group at 48, 24 and 0 h before AI, and 1 h after AI. Both 2CN and eCG treatments similarlyimproved sexual receptivity (76.3, 77.5 and 58.2{\%}, respectively; P < 0.001) and fertility (63.1, 64.1 and48.4{\%}, respectively; P < 0.05) in lactating does, compared to the CONTROL group. Similar plasma FSHlevels in all groups of does and sampling times were observed. Due to the absence of suckling, plasmaconcentration of PRL on day 10 post-partum in the 2CN group was lower than in the CONTROL group(P < 0.05); this endocrine change in PRL levels could explain the better reproductive performances obtained with 2CN treatment. At 1 h after exogenous administration of GnRH (at the moment of AI) a high LHresponse was observed in all groups (P < 0.001).Ovaries from 20 rabbits treated in the same way but uninseminated (2CN, n = 10; eCG, n = 5; CONTROL,n = 5 does) were obtained on day 11 post-partum in order to check the morphometric status (weight, width andheight) and to make histological and immunohistochemical studies to detect growth hormone receptor (GHR).As a result, synchronization methods did not showany significant difference in relation to the CONTROLgroup. However, a small increase in the number of primary follicles was evidenced in the 2CN group withrespect to the eCG group, similarly to the CONTROL group (23.0±3.7, 9.4±4.9 and 14.8±4.92 primaryfollicles, respectively; P = 0.1). GH-R immunostaining-presence was more evident in the 2CN and the eCGgroups, including primordial follicles and oocytes themselves. Thus, there could have been some direct effectsofGHon follicular development, as described in other species. Some ovarian parameters described open newways to study intra-ovarian mechanism of follicular development in the post-partum period of rabbit does.",
keywords = "FSH, Follicles, GH, LH, Lactation, Ovary, PRL, Rabbit, Synchronization",
author = "{Di Grigoli}, Antonino and Adriana Bonanno and Gabriele Tornamb{\`e} and Tornamb{\`e} and Bonanno and Rebollar and Lorenzo and {Di Grigoli}",
year = "2008",
language = "English",
volume = "104",
pages = "316--328",
journal = "Animal Reproduction Science",
issn = "0378-4320",
publisher = "Elsevier",

}

TY - JOUR

T1 - Endocrine and ovarian response after a 2-daycontrolled suckling and eCG treatment inlactating rabbit does

AU - Di Grigoli, Antonino

AU - Bonanno, Adriana

AU - Tornambè, Gabriele

AU - Tornambè, null

AU - Bonanno, null

AU - Rebollar, null

AU - Lorenzo, null

AU - Di Grigoli, null

PY - 2008

Y1 - 2008

N2 - Synchronization methods are used to obtain higher fertility when artificial insemination (AI) is applied tolactating rabbit does. The most common methods are eCG administration or temporary doe–litter separation.Nevertheless, drawbacks have been reported, such as negative side effects of hormonal treatment in the doeand low litter growth due to absence of suckling, respectively. Recently, improved reproductive performance(without visible consequences on young rabbit growth), has been obtained by applying a 2-day controllednursing method before AI, by allowing for a 10 min nursing of the litter 24 h of separation. The presentstudy was undertaken to examine the pituitary (PRL, LH, FSH) and the ovarian response (follicle size andnumber) to those methods. A total of 442 lactating does inseminated on day 11 post-partum were distributedin three experimental groups: 2CN (closing of nest box on day 9, controlled nursing on days 10 and 11),eCG (20 IU administered on day 9 post-partum) and CONTROL (untreated). Blood samples were obtainedfrom 10 does per group at 48, 24 and 0 h before AI, and 1 h after AI. Both 2CN and eCG treatments similarlyimproved sexual receptivity (76.3, 77.5 and 58.2%, respectively; P < 0.001) and fertility (63.1, 64.1 and48.4%, respectively; P < 0.05) in lactating does, compared to the CONTROL group. Similar plasma FSHlevels in all groups of does and sampling times were observed. Due to the absence of suckling, plasmaconcentration of PRL on day 10 post-partum in the 2CN group was lower than in the CONTROL group(P < 0.05); this endocrine change in PRL levels could explain the better reproductive performances obtained with 2CN treatment. At 1 h after exogenous administration of GnRH (at the moment of AI) a high LHresponse was observed in all groups (P < 0.001).Ovaries from 20 rabbits treated in the same way but uninseminated (2CN, n = 10; eCG, n = 5; CONTROL,n = 5 does) were obtained on day 11 post-partum in order to check the morphometric status (weight, width andheight) and to make histological and immunohistochemical studies to detect growth hormone receptor (GHR).As a result, synchronization methods did not showany significant difference in relation to the CONTROLgroup. However, a small increase in the number of primary follicles was evidenced in the 2CN group withrespect to the eCG group, similarly to the CONTROL group (23.0±3.7, 9.4±4.9 and 14.8±4.92 primaryfollicles, respectively; P = 0.1). GH-R immunostaining-presence was more evident in the 2CN and the eCGgroups, including primordial follicles and oocytes themselves. Thus, there could have been some direct effectsofGHon follicular development, as described in other species. Some ovarian parameters described open newways to study intra-ovarian mechanism of follicular development in the post-partum period of rabbit does.

AB - Synchronization methods are used to obtain higher fertility when artificial insemination (AI) is applied tolactating rabbit does. The most common methods are eCG administration or temporary doe–litter separation.Nevertheless, drawbacks have been reported, such as negative side effects of hormonal treatment in the doeand low litter growth due to absence of suckling, respectively. Recently, improved reproductive performance(without visible consequences on young rabbit growth), has been obtained by applying a 2-day controllednursing method before AI, by allowing for a 10 min nursing of the litter 24 h of separation. The presentstudy was undertaken to examine the pituitary (PRL, LH, FSH) and the ovarian response (follicle size andnumber) to those methods. A total of 442 lactating does inseminated on day 11 post-partum were distributedin three experimental groups: 2CN (closing of nest box on day 9, controlled nursing on days 10 and 11),eCG (20 IU administered on day 9 post-partum) and CONTROL (untreated). Blood samples were obtainedfrom 10 does per group at 48, 24 and 0 h before AI, and 1 h after AI. Both 2CN and eCG treatments similarlyimproved sexual receptivity (76.3, 77.5 and 58.2%, respectively; P < 0.001) and fertility (63.1, 64.1 and48.4%, respectively; P < 0.05) in lactating does, compared to the CONTROL group. Similar plasma FSHlevels in all groups of does and sampling times were observed. Due to the absence of suckling, plasmaconcentration of PRL on day 10 post-partum in the 2CN group was lower than in the CONTROL group(P < 0.05); this endocrine change in PRL levels could explain the better reproductive performances obtained with 2CN treatment. At 1 h after exogenous administration of GnRH (at the moment of AI) a high LHresponse was observed in all groups (P < 0.001).Ovaries from 20 rabbits treated in the same way but uninseminated (2CN, n = 10; eCG, n = 5; CONTROL,n = 5 does) were obtained on day 11 post-partum in order to check the morphometric status (weight, width andheight) and to make histological and immunohistochemical studies to detect growth hormone receptor (GHR).As a result, synchronization methods did not showany significant difference in relation to the CONTROLgroup. However, a small increase in the number of primary follicles was evidenced in the 2CN group withrespect to the eCG group, similarly to the CONTROL group (23.0±3.7, 9.4±4.9 and 14.8±4.92 primaryfollicles, respectively; P = 0.1). GH-R immunostaining-presence was more evident in the 2CN and the eCGgroups, including primordial follicles and oocytes themselves. Thus, there could have been some direct effectsofGHon follicular development, as described in other species. Some ovarian parameters described open newways to study intra-ovarian mechanism of follicular development in the post-partum period of rabbit does.

KW - FSH

KW - Follicles

KW - GH

KW - LH

KW - Lactation

KW - Ovary

KW - PRL

KW - Rabbit

KW - Synchronization

UR - http://hdl.handle.net/10447/36672

M3 - Article

VL - 104

SP - 316

EP - 328

JO - Animal Reproduction Science

JF - Animal Reproduction Science

SN - 0378-4320

ER -