TY - JOUR
T1 - Effects on lipoprotein subclasses of combined expression of human hepatic lipase and human apoB in transgenic rabbits
AU - Barbagallo, Carlo Maria
AU - Rizzo, Manfredi
AU - Taylor, John M.
AU - Berneis, Kaspar
AU - Krauss, Ronald M.
AU - Blanche, Patricia J.
AU - Rizzo, Manfredi
PY - 2004
Y1 - 2004
N2 - Objective—The effects of combined expression of human hepatic lipase (HL) and human apolipoprotein B (apoB) onlow-density lipoprotein (LDL) subclasses were examined in rabbits, a species naturally deficient in HL activity.Methods and Results—In apoB-transgenic rabbit plasma, 80% of the protein was found in the 1.006- to 1.050-g/mLfraction. Gradient gel electrophoresis (GGE) of this fraction revealed two distinct species, designated large and smallLDL. A denser fraction (d 1.050 to 1.063 g/mL) contained small LDL as well as another discrete LDL subspecies,designated very small LDL. Expression of HL resulted in reductions in protein concentrations in the 1.006- to1.050-g/mL density-gradient subfractions containing large (6.5 4.1 versus 32.6 12.0 mg/dL, P 0.005) and smallLDL (59.6 17.4 versus 204.3 50.3 mg/dL, P 0.002). A concomitant small but not significant increase in proteinconcentration in the denser LDL fraction (48.0 28.2 versus 44.6 18.2 mg/dL) was due primarily to an increase in verysmall LDL (25.9 3.1 versus 9.6 5.4% of total LDL GGE densitometric area, P 0.002).Conclusion—These findings support a direct role for HL in regulating total plasma LDL concentrations as well as in theproduction of smaller, denser LDL from larger, more buoyant precursors.
AB - Objective—The effects of combined expression of human hepatic lipase (HL) and human apolipoprotein B (apoB) onlow-density lipoprotein (LDL) subclasses were examined in rabbits, a species naturally deficient in HL activity.Methods and Results—In apoB-transgenic rabbit plasma, 80% of the protein was found in the 1.006- to 1.050-g/mLfraction. Gradient gel electrophoresis (GGE) of this fraction revealed two distinct species, designated large and smallLDL. A denser fraction (d 1.050 to 1.063 g/mL) contained small LDL as well as another discrete LDL subspecies,designated very small LDL. Expression of HL resulted in reductions in protein concentrations in the 1.006- to1.050-g/mL density-gradient subfractions containing large (6.5 4.1 versus 32.6 12.0 mg/dL, P 0.005) and smallLDL (59.6 17.4 versus 204.3 50.3 mg/dL, P 0.002). A concomitant small but not significant increase in proteinconcentration in the denser LDL fraction (48.0 28.2 versus 44.6 18.2 mg/dL) was due primarily to an increase in verysmall LDL (25.9 3.1 versus 9.6 5.4% of total LDL GGE densitometric area, P 0.002).Conclusion—These findings support a direct role for HL in regulating total plasma LDL concentrations as well as in theproduction of smaller, denser LDL from larger, more buoyant precursors.
UR - http://hdl.handle.net/10447/14748
M3 - Article
VL - 24(1)
SP - 141
EP - 146
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
ER -