Effects on lipoprotein subclasses of combined expression of human hepatic lipase and human apoB in transgenic rabbits

Carlo Maria Barbagallo, Manfredi Rizzo, John M. Taylor, Kaspar Berneis, Ronald M. Krauss, Patricia J. Blanche, Manfredi Rizzo

Risultato della ricerca: Articlepeer review

33 Citazioni (Scopus)

Abstract

Objective—The effects of combined expression of human hepatic lipase (HL) and human apolipoprotein B (apoB) onlow-density lipoprotein (LDL) subclasses were examined in rabbits, a species naturally deficient in HL activity.Methods and Results—In apoB-transgenic rabbit plasma, 80% of the protein was found in the 1.006- to 1.050-g/mLfraction. Gradient gel electrophoresis (GGE) of this fraction revealed two distinct species, designated large and smallLDL. A denser fraction (d 1.050 to 1.063 g/mL) contained small LDL as well as another discrete LDL subspecies,designated very small LDL. Expression of HL resulted in reductions in protein concentrations in the 1.006- to1.050-g/mL density-gradient subfractions containing large (6.5 4.1 versus 32.6 12.0 mg/dL, P 0.005) and smallLDL (59.6 17.4 versus 204.3 50.3 mg/dL, P 0.002). A concomitant small but not significant increase in proteinconcentration in the denser LDL fraction (48.0 28.2 versus 44.6 18.2 mg/dL) was due primarily to an increase in verysmall LDL (25.9 3.1 versus 9.6 5.4% of total LDL GGE densitometric area, P 0.002).Conclusion—These findings support a direct role for HL in regulating total plasma LDL concentrations as well as in theproduction of smaller, denser LDL from larger, more buoyant precursors.
Lingua originaleEnglish
pagine (da-a)141-146
RivistaArteriosclerosis, Thrombosis, and Vascular Biology
Volume24(1)
Stato di pubblicazionePublished - 2004

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

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