Previously we showed apoptotic induction in A375 human melanoma cells using two complexes of the mesotetra(4-sulfonatophenyl)porphinate (TPPS),(Bu2Sn)2TPPS and (Bu3Sn)4TPPS.To understand how these compoundsactivate apoptosis in melanoma cells we studied MAPKs and the (Bu2Sn)TPPS and (Bu3Sn)4TPPS cellular uptake.Western blotting experiments showed activated protein kinases ERK 1/2, JNK and p38 in 10 μM (Bu2Sn)2TPPS- and 1 μM (Bu3Sn)4TPPS-treated melanoma cells, which suggests that the three MAP kinases are involved in the apoptotic death of A375-treated cells. By taking advantage of the porphyrinfluorescence, we found a fast concentration of (Bu2Sn)2TPPS and (Bu3Sn)4TPPS in the nucleus and in the nucleoli comparedto TPPS. A significantly reduced growth of A375 human melanoma cells was also observed after only 48 h treatment by using 500 nM of (Bu2Sn)2TPPS or 80 nM of (Bu3Sn)4TPPS.A strong slowdown of cell growth and loss of cell-cell interactions were visible by in vitro wound repair assay.
|Numero di pagine||8|
|Stato di pubblicazione||Published - 2009|
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