Effects of tributyltin chloride in ascidian embryos: modulation of kinase-mediated signalling pathways

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    Abstract

    We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.
    Lingua originaleEnglish
    pagine (da-a)S87-S94
    Numero di pagine0
    RivistaInvertebrate Survival Journal
    Volume6
    Stato di pubblicazionePublished - 2009

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    Ascidiacea
    mitogen-activated protein kinase
    embryo (animal)
    phosphotransferases (kinases)
    tyrosine
    phosphorylation
    protein phosphorylation
    xenobiotics
    immunoblotting
    signal transduction
    tri-n-butyltin hydride
    proteins

    All Science Journal Classification (ASJC) codes

    • Animal Science and Zoology

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    @article{f1d8b999380147369f55d867c91294d7,
    title = "Effects of tributyltin chloride in ascidian embryos: modulation of kinase-mediated signalling pathways",
    abstract = "We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.",
    keywords = "tributyltin-induced effect; tyrosine kinase signalling; MAPK; ERK (p44/42); ascidian embryos",
    author = "Mario Gianguzza and Giuseppe Dolcemascolo and Francesca Damiani",
    year = "2009",
    language = "English",
    volume = "6",
    pages = "S87--S94",
    journal = "Invertebrate Survival Journal",
    issn = "1824-307X",
    publisher = "University of Modena and Reggio Emilia",

    }

    TY - JOUR

    T1 - Effects of tributyltin chloride in ascidian embryos: modulation of kinase-mediated signalling pathways

    AU - Gianguzza, Mario

    AU - Dolcemascolo, Giuseppe

    AU - Damiani, Francesca

    PY - 2009

    Y1 - 2009

    N2 - We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.

    AB - We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.

    KW - tributyltin-induced effect; tyrosine kinase signalling; MAPK; ERK (p44/42); ascidian embryos

    UR - http://hdl.handle.net/10447/44404

    M3 - Article

    VL - 6

    SP - S87-S94

    JO - Invertebrate Survival Journal

    JF - Invertebrate Survival Journal

    SN - 1824-307X

    ER -