Abstract
| Lingua originale | English |
|---|---|
| pagine (da-a) | S87-S94 |
| Numero di pagine | 0 |
| Rivista | Invertebrate Survival Journal |
| Volume | 6 |
| Stato di pubblicazione | Published - 2009 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Animal Science and Zoology
Cita questo
Effects of tributyltin chloride in ascidian embryos: modulation of kinase-mediated signalling pathways. / Gianguzza, Mario; Dolcemascolo, Giuseppe; Damiani, Francesca.
In: Invertebrate Survival Journal, Vol. 6, 2009, pag. S87-S94.Risultato della ricerca: Article
}
TY - JOUR
T1 - Effects of tributyltin chloride in ascidian embryos: modulation of kinase-mediated signalling pathways
AU - Gianguzza, Mario
AU - Dolcemascolo, Giuseppe
AU - Damiani, Francesca
PY - 2009
Y1 - 2009
N2 - We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.
AB - We studied the effects of various TBT concentrations by assaying the activity of ERK 1/2 (p44/42) and phospho-ERK1/2 (phospho-p44/42), proteins with a key role in ascidian development, and tyrosine kinase-dependent pathway. The effects of this xenobiotic and the role of some signalling mechanisms on ascidian embryos were examined by using Western immunoblotting. The tyrosine phosphorylation pattern in the ascidians Ciona intestinalis and Phallusia mammillata development was examined and different levels of protein phosphorylation were found as a response to TBT at μM range. To determine whether another key signalling pathway was activated, the effects of TBT on the phosphorylation state of a component of tyrosine kinase-mediated signal transduction MAPK, ERK 1/2 (p44/42) were evaluated. Embryos of Ciona intestinalis exposed to 0.1, 0.25 and 0.5 μM TBT showed a slight decrement in the level of phosphorylated ERK, while a remarkable decrement in level of phopshorylated ERK were observed at higher TBT concentrations (0.5 μM to 10 μM). These data indicated that exposures to TBT induced changes in the total pattern of phosphotyrosine and in the phosphorylation levels of ERK 1/2 but there were no changes on the overall level of total ERK in ascidian embryos.
KW - tributyltin-induced effect; tyrosine kinase signalling; MAPK; ERK (p44/42); ascidian embryos
UR - http://hdl.handle.net/10447/44404
M3 - Article
VL - 6
SP - S87-S94
JO - Invertebrate Survival Journal
JF - Invertebrate Survival Journal
SN - 1824-307X
ER -