Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae)

D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

The histopathological effects of 10−7 and 10−9 M tributyltin(IV)chloride,TBTCl, solutions on different Liza saliens organs have been studied by light microscope. The fish were sacrificed after 3–4 h incubation in 10−7 M TBTCl solution or after 15 days incubation in 10−9 M solution. The observed histopathological changes were dose- and time-dependent. The 10−7 M TBTCl concentration resulted in major damage to the gill epithelium, indicating that TBTCl primarily interferedwith the respiration, osmoregulation, acid balance and nitrogenous waste excretion processes. After incubation in 10−9 M TBTCl solution the fish lived 20 or more days, but many of the organs were altered. Thymus atrophy, reduced spleen and altered head kidney were observed. These histological results indicated that TBTCl interfered with organ immunodefense and altered main metabolic pathways in Liza saliens. The presence of melano-macrophage centers, only in TBT-treated liver and spleen, can be considered a tool to facilitate, with other biomarkers, the detection of alterations by toxicants. Regarding the pancreas activity in 10−7 M solutions, it has been noted that, in the exocrine cells, very few zymogen granuleswere still present and the Langerhans isletsweremore altered. In 10−9M solution the exocrine pancreatic cells had no granules and the islet cells presented degenerative alterations. In addition, TBTCl, which altered the pancreas and gonad morphology, could again be considered an endocrine disrupter even if biochemical data are still necessary. Finally, the Liza saliens juveniles could be considered an interesting biological model for experiments with contaminants, due to their ease of adaptation to experimental conditions and food chain position.
Lingua originaleEnglish
pagine (da-a)357-367
Numero di pagine11
RivistaApplied Organometallic Chemistry
Volume20(6)
Stato di pubblicazionePublished - 2006

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Toxicity
Fish
Thymus
Macrophages
Biomarkers
Liver
tributyltin
Chlorides
Microscopes
Impurities
Acids
Experiments

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Inorganic Chemistry

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D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L (2006). Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae). Applied Organometallic Chemistry, 20(6), 357-367.

Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae). / D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L.

In: Applied Organometallic Chemistry, Vol. 20(6), 2006, pag. 357-367.

Risultato della ricerca: Article

D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L 2006, 'Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae)', Applied Organometallic Chemistry, vol. 20(6), pagg. 357-367.
D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L. Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae). Applied Organometallic Chemistry. 2006;20(6):357-367.
D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L. / Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae). In: Applied Organometallic Chemistry. 2006 ; Vol. 20(6). pagg. 357-367.
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abstract = "The histopathological effects of 10−7 and 10−9 M tributyltin(IV)chloride,TBTCl, solutions on different Liza saliens organs have been studied by light microscope. The fish were sacrificed after 3–4 h incubation in 10−7 M TBTCl solution or after 15 days incubation in 10−9 M solution. The observed histopathological changes were dose- and time-dependent. The 10−7 M TBTCl concentration resulted in major damage to the gill epithelium, indicating that TBTCl primarily interferedwith the respiration, osmoregulation, acid balance and nitrogenous waste excretion processes. After incubation in 10−9 M TBTCl solution the fish lived 20 or more days, but many of the organs were altered. Thymus atrophy, reduced spleen and altered head kidney were observed. These histological results indicated that TBTCl interfered with organ immunodefense and altered main metabolic pathways in Liza saliens. The presence of melano-macrophage centers, only in TBT-treated liver and spleen, can be considered a tool to facilitate, with other biomarkers, the detection of alterations by toxicants. Regarding the pancreas activity in 10−7 M solutions, it has been noted that, in the exocrine cells, very few zymogen granuleswere still present and the Langerhans isletsweremore altered. In 10−9M solution the exocrine pancreatic cells had no granules and the islet cells presented degenerative alterations. In addition, TBTCl, which altered the pancreas and gonad morphology, could again be considered an endocrine disrupter even if biochemical data are still necessary. Finally, the Liza saliens juveniles could be considered an interesting biological model for experiments with contaminants, due to their ease of adaptation to experimental conditions and food chain position.",
author = "{D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L} and Caterina Mansueto and Lorenzo Pellerito and Tiziana Fiore and Claudia Pellerito and Paolo D'Agati and Cangialosi, {Maria Vittoria} and Michelangelo Scopelliti and Valentina Mansueto",
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T1 - Effects of sublethal levels of tributyltin chloride on a new toxicity test organism, Liza saliens (Osteichthyes, Mugilidae)

AU - D'Agati P; Mansueto C; Mansueto V; Pellerito C; Cangialosi Mv; Fiore T; Scopelliti M; Pellerito L

AU - Mansueto, Caterina

AU - Pellerito, Lorenzo

AU - Fiore, Tiziana

AU - Pellerito, Claudia

AU - D'Agati, Paolo

AU - Cangialosi, Maria Vittoria

AU - Scopelliti, Michelangelo

AU - Mansueto, Valentina

PY - 2006

Y1 - 2006

N2 - The histopathological effects of 10−7 and 10−9 M tributyltin(IV)chloride,TBTCl, solutions on different Liza saliens organs have been studied by light microscope. The fish were sacrificed after 3–4 h incubation in 10−7 M TBTCl solution or after 15 days incubation in 10−9 M solution. The observed histopathological changes were dose- and time-dependent. The 10−7 M TBTCl concentration resulted in major damage to the gill epithelium, indicating that TBTCl primarily interferedwith the respiration, osmoregulation, acid balance and nitrogenous waste excretion processes. After incubation in 10−9 M TBTCl solution the fish lived 20 or more days, but many of the organs were altered. Thymus atrophy, reduced spleen and altered head kidney were observed. These histological results indicated that TBTCl interfered with organ immunodefense and altered main metabolic pathways in Liza saliens. The presence of melano-macrophage centers, only in TBT-treated liver and spleen, can be considered a tool to facilitate, with other biomarkers, the detection of alterations by toxicants. Regarding the pancreas activity in 10−7 M solutions, it has been noted that, in the exocrine cells, very few zymogen granuleswere still present and the Langerhans isletsweremore altered. In 10−9M solution the exocrine pancreatic cells had no granules and the islet cells presented degenerative alterations. In addition, TBTCl, which altered the pancreas and gonad morphology, could again be considered an endocrine disrupter even if biochemical data are still necessary. Finally, the Liza saliens juveniles could be considered an interesting biological model for experiments with contaminants, due to their ease of adaptation to experimental conditions and food chain position.

AB - The histopathological effects of 10−7 and 10−9 M tributyltin(IV)chloride,TBTCl, solutions on different Liza saliens organs have been studied by light microscope. The fish were sacrificed after 3–4 h incubation in 10−7 M TBTCl solution or after 15 days incubation in 10−9 M solution. The observed histopathological changes were dose- and time-dependent. The 10−7 M TBTCl concentration resulted in major damage to the gill epithelium, indicating that TBTCl primarily interferedwith the respiration, osmoregulation, acid balance and nitrogenous waste excretion processes. After incubation in 10−9 M TBTCl solution the fish lived 20 or more days, but many of the organs were altered. Thymus atrophy, reduced spleen and altered head kidney were observed. These histological results indicated that TBTCl interfered with organ immunodefense and altered main metabolic pathways in Liza saliens. The presence of melano-macrophage centers, only in TBT-treated liver and spleen, can be considered a tool to facilitate, with other biomarkers, the detection of alterations by toxicants. Regarding the pancreas activity in 10−7 M solutions, it has been noted that, in the exocrine cells, very few zymogen granuleswere still present and the Langerhans isletsweremore altered. In 10−9M solution the exocrine pancreatic cells had no granules and the islet cells presented degenerative alterations. In addition, TBTCl, which altered the pancreas and gonad morphology, could again be considered an endocrine disrupter even if biochemical data are still necessary. Finally, the Liza saliens juveniles could be considered an interesting biological model for experiments with contaminants, due to their ease of adaptation to experimental conditions and food chain position.

UR - http://hdl.handle.net/10447/8907

M3 - Article

VL - 20(6)

SP - 357

EP - 367

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

SN - 0268-2605

ER -