Effects of sodium fluoride on the mechanical activity in mouse gastric preparations

Flavia Mule', Smain Amira

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Abstract

The aim of the present study was to investigate the responses induced by sodium fluoride (NaF) on gastric mechanical activity, using mouse whole-stomach preparations. The mechanical activity was recorded in vitro as changes of intraluminal pressure. In most of the preparations, NaF induced a tetrodotoxin-insensitive biphasic effect characterized by early relaxation followed by slowly developing contractile response. The contraction was dependent on the concentration of NaF, whereas the relaxation was observed at only 10–30 mmol/L NaF. The contractile effect was significantly reduced by nifedipine (an L-type Ca2+ channel blocker), ryanodine or ruthenium red (inhibitors of Ca2+ release from sarcoplasmic reticulum), and GF109203X (a protein kinase C inhibitor). Moreover, it was abolished by neomycin (an inhibitor of phospholipase C) and potentiated by SQ22536 (an inhibitor of adenylyl cyclase). All the drugs significantly increased the relaxation, except SQ22536, which abolished it. The present results suggest that NaF causes a complex mechanical response in the whole-stomach, which might explain gastric discomfort after fluoride ingestion. The relaxation appears owing to production of cAMP, while the contractile effects imply activation of phospholipase C, protein kinase C, influx of Ca2+, and release of Ca2+ from ryanodine-sensitive intracellular store.Key words: gastric mechanical activity, mouse stomach, smooth muscle, sodium fluoride.
Lingua originaleEnglish
pagine (da-a)367-373
Numero di pagine7
RivistaCanadian Journal of Physiology and Pharmacology
Volume83
Stato di pubblicazionePublished - 2005

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)
  • Pharmacology

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title = "Effects of sodium fluoride on the mechanical activity in mouse gastric preparations",
abstract = "The aim of the present study was to investigate the responses induced by sodium fluoride (NaF) on gastric mechanical activity, using mouse whole-stomach preparations. The mechanical activity was recorded in vitro as changes of intraluminal pressure. In most of the preparations, NaF induced a tetrodotoxin-insensitive biphasic effect characterized by early relaxation followed by slowly developing contractile response. The contraction was dependent on the concentration of NaF, whereas the relaxation was observed at only 10–30 mmol/L NaF. The contractile effect was significantly reduced by nifedipine (an L-type Ca2+ channel blocker), ryanodine or ruthenium red (inhibitors of Ca2+ release from sarcoplasmic reticulum), and GF109203X (a protein kinase C inhibitor). Moreover, it was abolished by neomycin (an inhibitor of phospholipase C) and potentiated by SQ22536 (an inhibitor of adenylyl cyclase). All the drugs significantly increased the relaxation, except SQ22536, which abolished it. The present results suggest that NaF causes a complex mechanical response in the whole-stomach, which might explain gastric discomfort after fluoride ingestion. The relaxation appears owing to production of cAMP, while the contractile effects imply activation of phospholipase C, protein kinase C, influx of Ca2+, and release of Ca2+ from ryanodine-sensitive intracellular store.Key words: gastric mechanical activity, mouse stomach, smooth muscle, sodium fluoride.",
author = "Flavia Mule' and Smain Amira",
year = "2005",
language = "English",
volume = "83",
pages = "367--373",
journal = "Canadian Journal of Physiology and Pharmacology",
issn = "0008-4212",
publisher = "National Research Council of Canada",

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TY - JOUR

T1 - Effects of sodium fluoride on the mechanical activity in mouse gastric preparations

AU - Mule', Flavia

AU - Amira, Smain

PY - 2005

Y1 - 2005

N2 - The aim of the present study was to investigate the responses induced by sodium fluoride (NaF) on gastric mechanical activity, using mouse whole-stomach preparations. The mechanical activity was recorded in vitro as changes of intraluminal pressure. In most of the preparations, NaF induced a tetrodotoxin-insensitive biphasic effect characterized by early relaxation followed by slowly developing contractile response. The contraction was dependent on the concentration of NaF, whereas the relaxation was observed at only 10–30 mmol/L NaF. The contractile effect was significantly reduced by nifedipine (an L-type Ca2+ channel blocker), ryanodine or ruthenium red (inhibitors of Ca2+ release from sarcoplasmic reticulum), and GF109203X (a protein kinase C inhibitor). Moreover, it was abolished by neomycin (an inhibitor of phospholipase C) and potentiated by SQ22536 (an inhibitor of adenylyl cyclase). All the drugs significantly increased the relaxation, except SQ22536, which abolished it. The present results suggest that NaF causes a complex mechanical response in the whole-stomach, which might explain gastric discomfort after fluoride ingestion. The relaxation appears owing to production of cAMP, while the contractile effects imply activation of phospholipase C, protein kinase C, influx of Ca2+, and release of Ca2+ from ryanodine-sensitive intracellular store.Key words: gastric mechanical activity, mouse stomach, smooth muscle, sodium fluoride.

AB - The aim of the present study was to investigate the responses induced by sodium fluoride (NaF) on gastric mechanical activity, using mouse whole-stomach preparations. The mechanical activity was recorded in vitro as changes of intraluminal pressure. In most of the preparations, NaF induced a tetrodotoxin-insensitive biphasic effect characterized by early relaxation followed by slowly developing contractile response. The contraction was dependent on the concentration of NaF, whereas the relaxation was observed at only 10–30 mmol/L NaF. The contractile effect was significantly reduced by nifedipine (an L-type Ca2+ channel blocker), ryanodine or ruthenium red (inhibitors of Ca2+ release from sarcoplasmic reticulum), and GF109203X (a protein kinase C inhibitor). Moreover, it was abolished by neomycin (an inhibitor of phospholipase C) and potentiated by SQ22536 (an inhibitor of adenylyl cyclase). All the drugs significantly increased the relaxation, except SQ22536, which abolished it. The present results suggest that NaF causes a complex mechanical response in the whole-stomach, which might explain gastric discomfort after fluoride ingestion. The relaxation appears owing to production of cAMP, while the contractile effects imply activation of phospholipase C, protein kinase C, influx of Ca2+, and release of Ca2+ from ryanodine-sensitive intracellular store.Key words: gastric mechanical activity, mouse stomach, smooth muscle, sodium fluoride.

UR - http://hdl.handle.net/10447/12185

M3 - Article

VL - 83

SP - 367

EP - 373

JO - Canadian Journal of Physiology and Pharmacology

JF - Canadian Journal of Physiology and Pharmacology

SN - 0008-4212

ER -