TY - JOUR
T1 - Effects of Nandrolone Stimulation on Testosterone Biosynthesis in Leydig Cells
AU - Sangiorgi, Claudia
AU - Di Gaudio, Francesca
AU - Di Felice, Valentina
AU - Marino Gammazza, Antonella
AU - Pitruzzella, Alessandro
AU - Barone, Rosario
AU - Cappello, Francesco
AU - Marino Gammazza, Antonella
AU - Pitruzzella, Alessandro
AU - Locorotondo, Nicola
AU - Turillazzi, Emanuela
AU - Barone, Rosario
AU - Maglietta, Francesca
AU - Salerno, Monica
AU - Barone, Fulvio
AU - Sarni, Antonio Luciano
AU - Cappello, Francesco
AU - Pomara, Cristoforo
PY - 2016
Y1 - 2016
N2 - Anabolic androgenic steroids (AAS) are among the drugs most used by athletes for improving physical performance, as well as for aesthetic purposes. A number of papers have showed the side effects of AAS in different organs and tissues. For example, AAS are known to suppress gonadotropin-releasing hormone, luteinizing hormone, and follicle-stimulating hormone. This study investigates the effects of nandrolone on testosterone biosynthesis in Leydig cells using various methods, including mass spectrometry, western blotting, confocal microscopy and quantitative real-time PCR. The results obtained show that testosterone levels increase at a 3.9μM concentration of nandrolone and return to the basal level a 15.6μM dose of nandrolone. Nandrolone-induced testosterone increment was associated with upregulation of the steroidogenic acute regulatory protein (StAR) and downregulation of 17a-hydroxylase/17, 20 lyase (CYP17A1). Instead, a 15.6μM dose of nandrolone induced a down-regulation of CYP17A1. Further in vivo studies based on these data are needed to better understand the relationship between disturbed testosterone homeostasis and reproductive system impairment in male subjects.
AB - Anabolic androgenic steroids (AAS) are among the drugs most used by athletes for improving physical performance, as well as for aesthetic purposes. A number of papers have showed the side effects of AAS in different organs and tissues. For example, AAS are known to suppress gonadotropin-releasing hormone, luteinizing hormone, and follicle-stimulating hormone. This study investigates the effects of nandrolone on testosterone biosynthesis in Leydig cells using various methods, including mass spectrometry, western blotting, confocal microscopy and quantitative real-time PCR. The results obtained show that testosterone levels increase at a 3.9μM concentration of nandrolone and return to the basal level a 15.6μM dose of nandrolone. Nandrolone-induced testosterone increment was associated with upregulation of the steroidogenic acute regulatory protein (StAR) and downregulation of 17a-hydroxylase/17, 20 lyase (CYP17A1). Instead, a 15.6μM dose of nandrolone induced a down-regulation of CYP17A1. Further in vivo studies based on these data are needed to better understand the relationship between disturbed testosterone homeostasis and reproductive system impairment in male subjects.
UR - http://hdl.handle.net/10447/198959
M3 - Article
SN - 0021-9541
VL - 231
SP - 1385
EP - 1391
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
ER -