Abstract

The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.
Lingua originaleEnglish
pagine (da-a)34-37
Numero di pagine4
RivistaJOURNAL OF BIOLOGICAL RESEARCH
Volume82
Stato di pubblicazionePublished - 2009

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HL-60 Cells
Phenols
Anthracyclines
Complex Mixtures
Cell Line
Surface Antigens
Apoptosis
Multiple Drug Resistance
Pharmaceutical Preparations
Inhibitory Concentration 50
Olive Oil

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title = "Effects of extra virgin olive oil phenols on HL60 cell lines sensitive and resistant to anthracycline",
abstract = "The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.",
keywords = "Phenols, apoptosis, differentiation, human cancer cell lines",
author = "Tumminello, {Francesca Maria} and Marilena Crescimanno and Carla Flandina and Gaetano Leto and Marco Giammanco and {Di Majo}, Danila and Elisa Tripoli and Sepporta, {Maria Vittoria}",
year = "2009",
language = "English",
volume = "82",
pages = "34--37",
journal = "Journal of Biological Research (Italy)",
issn = "1826-8838",
publisher = "PagePress Publications",

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TY - JOUR

T1 - Effects of extra virgin olive oil phenols on HL60 cell lines sensitive and resistant to anthracycline

AU - Tumminello, Francesca Maria

AU - Crescimanno, Marilena

AU - Flandina, Carla

AU - Leto, Gaetano

AU - Giammanco, Marco

AU - Di Majo, Danila

AU - Tripoli, Elisa

AU - Sepporta, Maria Vittoria

PY - 2009

Y1 - 2009

N2 - The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.

AB - The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.

KW - Phenols, apoptosis, differentiation, human cancer cell lines

UR - http://hdl.handle.net/10447/44965

M3 - Article

VL - 82

SP - 34

EP - 37

JO - Journal of Biological Research (Italy)

JF - Journal of Biological Research (Italy)

SN - 1826-8838

ER -