Abstract

The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.
Lingua originaleEnglish
pagine (da-a)34-37
Numero di pagine4
RivistaDefault journal
Volume82
Stato di pubblicazionePublished - 2009

Fingerprint

HL-60 Cells
Phenols
Anthracyclines
Complex Mixtures
Cell Line
Surface Antigens
Apoptosis
Multiple Drug Resistance
Pharmaceutical Preparations
Inhibitory Concentration 50
Olive Oil

Cita questo

@article{6ee4b4899ddf4e248fcdd880876b9d71,
title = "Effects of extra virgin olive oil phenols on HL60 cell lines sensitive and resistant to anthracycline",
abstract = "The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.",
keywords = "Phenols, apoptosis, differentiation, human cancer cell lines",
author = "{La Guardia}, Maurizio and Gaetano Leto and Marilena Crescimanno and Tumminello, {Francesca Maria} and {Di Majo}, Danila and Carla Flandina and Marco Giammanco and Elisa Tripoli and Sepporta, {Maria Vittoria}",
year = "2009",
language = "English",
volume = "82",
pages = "34--37",
journal = "Default journal",

}

TY - JOUR

T1 - Effects of extra virgin olive oil phenols on HL60 cell lines sensitive and resistant to anthracycline

AU - La Guardia, Maurizio

AU - Leto, Gaetano

AU - Crescimanno, Marilena

AU - Tumminello, Francesca Maria

AU - Di Majo, Danila

AU - Flandina, Carla

AU - Giammanco, Marco

AU - Tripoli, Elisa

AU - Sepporta, Maria Vittoria

PY - 2009

Y1 - 2009

N2 - The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.

AB - The aim of our study was to evaluate the capability of a crude extract of phenols from extra virgin olive oil of Moraiolo cultivar to induce apoptosis and/or differentiation in sensitive and resistant HL60 cell lines to anticancer drugs (Typical Multidrug Resistance). Our data highlight that the crude extract is able to induce apoptosis on both sensitive and resistant cells, whereas the exposure to a number of anticancer drugs does not induce apoptosis in resistant cells. In differentiation experiments we investigated the capability of crude extract of phenols to induce the expression of CD11 granulocytic or CD14 monocytic cell surface antigen in sensitive and resistant HL60 cell lines. At IC50 dose level (17 µg/ml and 32 µg/ml respectively for sensitive and resistant cell lines), the crude extract induced differentiation associated with the expression of CD14 monocytic cell surface antigen either in sensitive or resistant cell lines but not that of CD11 granulocityc cell surface antigen. Further investigations are in progress to better clarify the mechanism by which olive oil phenols induce differentiation differentiation on this cell line.

KW - Phenols

KW - apoptosis

KW - differentiation

KW - human cancer cell lines

UR - http://hdl.handle.net/10447/44965

M3 - Article

VL - 82

SP - 34

EP - 37

JO - Default journal

JF - Default journal

ER -